The Effect of Uphill and Downhill Eerobic Exercises on Serum Apolipoprotein
A1 and Apolipoprotein B Levels and their Ratio in Patients with
Chronic Obstructive Pulmonary Disease

Yazdani, Rostam; Marefati, Hamid; Shahesmaeili, Armita; Bagheri, Alireza; Langari, Ahmad Alinaghi; Nakhaei, Samira; Amiri, Mahmoud; Kameshki, Halimeh

doi:10.2174/1573398x17666210831160728

Cited by 5 publications

(5 citation statements)

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“…In the present study, AT + DE reduced plasma Apo B level probably by reducing the production of Apo B‐containing lipoproteins. Apo A‐1 is a critical component of HDL‐C and the previous studies showed that long‐term exercise training increased the synthesis of Apo A‐1 and reduced its catabolism (Whyte et al, 2020; Yazdani et al, 2018). However, in this research, there was no significant increase in Apo A‐1.…”

Section: Discussionmentioning

confidence: 99%

Hydroalcoholic extract of dill and aerobic training prevents high‐fat diet‐induced metabolic risk factors by improving miR ‐33 and miR ‐223 expression in rat liver

Aliakbari

Saghebjoo

Sarir

et al. 2022

Journal of Food Biochemistry

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Exercise training and medicinal herb supplementation may improve microRNAs (miRNAs) expression associated with obesity. This study aimed to assess the effects of 10 weeks of aerobic training (AT) and dill extract (DE) on miR‐33 and miR‐223 expression of liver in high‐fat diet (HFD)‐induced obese rats. Forty male Wistar rats were fed a defined high‐fat (n = 32) and standard (n = 8, nonobese control [NC]) diet. After obesity induction, obese rats were randomly allocated to four groups: AT, DE, AT + DE, and obese control (OC). Rats were euthanized and plasma and liver tissue samples were collected after the intervention. The liver expression of miR‐33 was lower in the AT, DE, AT + DE, and NC groups compared with the OC group. Also, the liver miR‐223 expression was higher in the AT, DE, AT + DE, and NC groups compared with the OC group. Moreover, the liver expression of miR‐223 in the AT + DE group was higher compared with the AT and DE groups. The AT, DE, AT + DE, and NC groups had lower liver TC compared with the OC group. Also, the plasma level of apolipoprotein B (Apo B) was significantly lower, and liver HDL‐C was significantly higher in the AT + DE and NC groups compared with the OC group. These findings show that long‐term AT combined with the intake of DE may improve the plasma levels of Apo B, and TC and HDL‐C levels in the liver, which is probably due to AT and DE positive effects on miR‐33 and miR‐223 in the liver of obese rats. Practical applications Aerobic training reduces overweight and obesity health problems, however, the duration and intensity of the exercise training distinguish between individuals. We used an integrated approach combining pharmacological and non‐pharmacological as a medical strategy to prevent HFD‐induced metabolic injury in obese rats. The present results discovered that a combination of AT + DE intervention improves the miR‐33 and miR‐223 in the liver of obese rats.

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Section: Discussionmentioning

confidence: 99%

Hydroalcoholic extract of dill and aerobic training prevents high‐fat diet‐induced metabolic risk factors by improving miR ‐33 and miR ‐223 expression in rat liver

Aliakbari

Saghebjoo

Sarir

et al. 2022

Journal of Food Biochemistry

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“…Han et al showed that ApoA1 can stimulate glucose uptake in C2C12 myotubes [65]. Furthermore, previous studies have shown that exercise increases levels of ApoA1 in humans [66], [67]. The discrepancy in results could be the due to the type of exercise/species model used – we used an in vitro model of chronic stimulation using murine cells, whereas others used plasma from humans after weeks of aerobic exercise.…”

Section: Discussionmentioning

confidence: 99%

Skeletal muscle-derived extracellular vesicles are altered with chronic contractile activity

Patience

Seif

Bydak

et al. 2022

Preprint

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Extracellular vesicles (EVs) are small lipid membrane-bound structures that are secreted by all cells, and play a central role in cellular communication. EVs are released from skeletal muscle during exercise, but the effects of contractile activity on skeletal muscle-derived EVs (Skm-EVs) are poorly understood due to the challenges in distinguishing Skm-EVs derived from exercising muscle in vivo. To specifically characterize Skm-EVs, C2C12 myoblasts were differentiated into myotubes, and electrically paced (3h/day x 4days @14V, C-PACE EM, IonOptix) to mimic chronic exercise in vitro. EVs were isolated from conditioned media from control and stimulated myotubes using differential ultracentrifugation. Isolated EVs were characterized biophysically (size, zeta potential, yield, protein markers and by transmission electron microscopy; TEM). Chronic stimulation increased markers of mitochondrial biogenesis such as MitoTracker Red staining, cytochrome c oxidase activity and expression of cytochrome c (p<0.05, N=7-8) in stimulated vs. non-stimulated myotubes. The average size of EVs from chronically stimulated myotubes (CS-EVs, 132 nm) was 26% smaller than control (CON-EVs, 178 nm) (p<0.05, N=8). Size distribution analysis revealed that CS-EVs were enriched with 100-150 nm sized small EVs, while CON-EVs were largely composed of 200-250 nm sized vesicles (p<0.05, main interaction effect, N=8). TEM confirmed the presence of round-shaped vesicles of about 30-100 nm with an intact lipid bilayer in CON-EVs and CS-EVs. Zeta potential was 27% lower in CS-EVs vs. CON-EVs (p<0.05, N=8), and total EV protein yield remained unchanged between groups. Protein-based EV characterization showed that both CON-EVs/CS-EVs were CD81+ but CD63-, and expressed hallmark cytosolic proteins recovered in EVs: Tsg101, Flotillin-1, HSP70, and Alix. CD81 and HSP70 expression increased in CS-EVs vs. CON-EVs (p<0.05, N=9). We evaluated if chronic stimulation affected whole cell expression of transmembrane and cytosolic proteins used in EV origin/purity analysis. CD63 and ApoA1 were reduced with chronic stimulation in myotube lysates (p<0.05, N=7), whereas Tsg101, CD81, Flotillin-1 and HSP70 levels remained constant. Taken together, our study revealed that chronic stimulation triggers the release of more stable, smaller sized EVs, enriched with transmembrane and cytosolic protein markers of small EVs, and are CD81+/CD63- indicating the origin of these EVs might be ectosomal rather than endosomal. The upstream signaling cascades that regulate biogenesis of EVs with chronic stimulation, and whether skeletal muscle-EVs are released through endosomal or ectosomal pathways remains to be elucidated. Our findings support the role of chronic contractile activity in the modulation of EV biophysical characteristics. Whether this affects biological cargo recruitment into CS-EVs, and their subsequent biological activity remains to be established.

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“…In particular, it cannot be proven that this mechanism directly reduces lipoprotein levels, which are risk factors of CVD. Following aerobic exercise, ApoA-I levels were elevated in patients with chronic obstructive pulmonary disease [ 64 ]. Moreover, in CVD, exercise training directly affected not only traditional cardiovascular risk factors, but also vascular function and structure [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Dynamic Resistance Exercise Alters Blood ApoA-I Levels, Inflammatory Markers, and Metabolic Syndrome Markers in Elderly Women

Ahn

Kim

2022

Healthcare

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Combined endurance and dynamic-resistance exercise has important anti-inflammatory effects, altering vascular endothelial function, and helping to prevent and treat aging-related metabolic syndrome (MS). We studied changes in 40 elderly women aged ≥ 65 years (control group (no MS), n = 20, mean age: 68.23 ± 2.56 years; MS group, n = 19, mean age: 71.42 ± 5.87 years; one left). The exercise program comprised dynamic-resistance training using elastic bands, three times weekly, for six months. We analyzed body composition, blood pressure, physical fitness, and MS-related blood variables including ApoA-I, antioxidant factors, and inflammatory markers. After the program, the MS group showed significant reductions in waist-hip ratio, waist circumference, diastolic blood pressure, blood insulin, and HOMA-IR, and a significant increase in HSP70 (p < 0.05). Both groups showed significant increases in ApoA-I levels, ApoA-I/HDL-C ratio, SOD2, IL-4, and IL-5 levels (p < 0.05). Active-resistance training-induced changes in ApoA-I were significantly positively correlated with changes in HDL-C and HSP70, and significantly negatively correlated with changes in triglycerides, C-reactive protein, and TNF-α (p < 0.05). Active-resistance training qualitatively altered HDL, mostly by altering ApoA-I levels, relieving vascular inflammation, and improving antioxidant function. This provides evidence that dynamic-resistance exercise can improve physical fitness and MS risk factors in elderly women.

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The Effect of Uphill and Downhill Eerobic Exercises on Serum Apolipoprotein A1 and Apolipoprotein B Levels and their Ratio in Patients with Chronic Obstructive Pulmonary Disease

Cited by 5 publications

References 0 publications

Hydroalcoholic extract of dill and aerobic training prevents high‐fat diet‐induced metabolic risk factors by improving miR ‐33 and miR ‐223 expression in rat liver

Hydroalcoholic extract of dill and aerobic training prevents high‐fat diet‐induced metabolic risk factors by improving miR ‐33 and miR ‐223 expression in rat liver

Skeletal muscle-derived extracellular vesicles are altered with chronic contractile activity

Dynamic Resistance Exercise Alters Blood ApoA-I Levels, Inflammatory Markers, and Metabolic Syndrome Markers in Elderly Women

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