The Effect of Unilateral Adrenalectomy on Transformation of Adrenal Medullary Chromaffin Cells In Vivo: A Potential Mechanism of Asthma Pathogenesis

Zou, Ye-Qiang; Feng, Juntao; Li, Xiaozhao

doi:10.1371/journal.pone.0044586

Cited by 10 publications

(14 citation statements)

References 23 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, OVA exposure dramatically elevated airway responsiveness even at low antigen concentration (16). In this study, we observed a significant increase in the infiltration of peribronchial inflammatory cells, particularly the lymphocytes in HiTr-treated rats.…”

Section: Discussionsupporting

confidence: 54%

“…In contrast, MoTr could upregulate EPI and CORT levels. This could explain the occurrence of airway hyperresponsiveness among endurance athletes after competitions because the blunted sympathoadrenal system is unable to release sufficient EPI to dilate the bronchus (12,16,33) after prolonged high-intensity training. The time required for rats to reach exhaustion was used to evaluate the efficacy of different training regimens in inducing exercise tolerance.…”

Section: L608 Adrenal Medulla Cells Conversion In Eibmentioning

confidence: 99%

See 1 more Smart Citation

High-intensity training induces EIB in rats through neuron transdifferentiation of adrenal medulla chromaffin cells

Feng

Xun

et al. 2013

American Journal of Physiology-Lung Cellular and Molecular Phys

View full text Add to dashboard Cite

He R, Feng J, Xun Q, Qin Q, Hu C. High-intensity training induces EIB in rats through neuron transdifferentiation of adrenal medulla chromaffin cells. Am J Physiol Lung Cell Mol Physiol 304: L602-L612, 2013. First published February 15, 2013 doi:10.1152/ajplung.00406.2012.-A high prevalence of exercise-induced bronchoconstriction (EIB) can be found in elite athletes, but the underlying mechanisms remain elusive. Airway responsiveness, NGF and epinephrine (EPI) levels, and chromaffin cell structure in high-(HiTr) and moderate-intensity training (MoTr) rats with or without ovalbumin (OVA) sensitization were measured in a total of 120 male Sprague-Dawley rats. The expression of NGF-associated genes in rat adrenal medulla was tested. Both HiTr and OVA intervention significantly increased airway resistance to aerosolized methacholine measured by whole body plethysmography. HiTr significantly increased inflammatory reaction in the lung with a major increase in peribronchial lymphocyte infiltration, whereas OVA significantly increased the infiltration of various inflammatory cells with an over 10-fold increase in eosinophil level in bronchoalveolar lavage. Both HiTr and OVA intervention upregulated circulating NGF level and peripherin level in adrenal medulla, but downregulated phenylethanolamine N-methyl transferase level in adrenal medulla and circulating EPI level. HiTr ϩ OVA and HiTr ϩ ExhEx (exhaustive exercise) interventions significantly enhanced most of the HiTr effects. The elevated NGF level was significantly associated with neuronal conversion of adrenal medulla chromaffin cells (AMCC). The levels of p-Erk1/2, JMJD3, and Mash1 were significantly increased, but the levels of p-p38 and p-JNK were significantly decreased in adrenal medulla in HiTr and OVA rats. Injection of NGF antiserum and moderate-intensity training reversed these changes observed in HiTr and/or OVA rats. Our study suggests that NGF may play a vital role in the pathogenesis of EIB by inducing neuron transdifferentiation of AMCC via MAPK pathways and subsequently decreasing circulating EPI. exercise; asthma; lung; inflammation; NGF; neuron conversion; adrenal medulla EXERCISE-INDUCED BRONCHOCONSTRICTION (EIB) is the increase in airway obstruction with symptoms of coughing, wheezing, or dyspnea as a result of exercise (23). EIB occurs in ϳ11% of the general population without asthma (13). Inhalation of cold air and sudden changes in humidity or temperature have been associated with EIB (20). High prevalence of EIB is usually observed among endurance athletes (7), implicating that intensity of exercise may be associated with EIB.Although thermal and osmotic consequences of water loss play prominent roles in the emergence of EIB (2), more studies are needed to elucidate the underlying mechanism. Circulating epinephrine (EPI), produced by adrenal medulla chromaffin cells (AMCC), is believed to bind the adrenergic receptors of airway smooth muscles to achieve adrenergic bronchodilation, and a loss of this function could directly influence the sev...

show abstract

Section: Discussionsupporting

confidence: 54%

Section: L608 Adrenal Medulla Cells Conversion In Eibmentioning

confidence: 99%

High-intensity training induces EIB in rats through neuron transdifferentiation of adrenal medulla chromaffin cells

Feng

Xun

et al. 2013

American Journal of Physiology-Lung Cellular and Molecular Phys

View full text Add to dashboard Cite

show abstract

“…The ultrathin tissue sections (50 nm) were stained with uranyl acetate and lead citrate, and finally examined under a H-7,500 transmission electron microscope (Hitachi, Tokyo, Japan). The densities of chromaffin granules per gland (/mm 3 ) were assessed for every rat (Hu et al 2012a). The numbers of neurite-bearing cells and AMCCs in the adrenal medulla of individual rats were counted and the percentages of neurite-bearing cells in the total numbers of cells were calculated, based on at least thirty cells from two sections of each sample and 4-5 rats per group.…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

“…Our previous studies have shown that adrenal medullary chromaffin cells (AMCCs) have a tendency to transform into neurons, which results in impaired endocrine function of the adrenal medulla, thereby leading to a decrease in the levels of circulating epinephrine in a rat model of asthma (Feng and Hu 2005;Feng et al 2012;Hu et al 2012a). In addition, we found that high-intensity exercise not only promoted inflammatory responses and increased the airway resistance, but also induced neuronal transdifferentiation of AMCCs and subsequently decreased the levels of circulating epinephrine while moderate-intensity of exercise reversed these changes (He et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Low-Intensity Aerobic Exercise Mitigates Exercise-Induced Bronchoconstriction by Improving the Function of Adrenal Medullary Chromaffin Cells in Asthmatic Rats

Qin

Feng

Chen

et al. 2014

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

Exercise is one of the most common triggers of bronchoconstriction in patients with asthma. The low levels of circulating epinephrine produced by the adrenal medullary chromaffin cells (AMCCs) are associated with exercise-induced bronchoconstriction (EIB) in asthmatics. In the present study, we tested the hypothesis that low-intensity aerobic exercise may ameliorate EIB using a rat model of asthma. Male Sprague-Dawley rats at 7 weeks of age, sensitized with ovalbumin or treated with saline, were subjected to low or moderate exercise training (50 or 75% of maximum velocity) for one hour in a treadmill 30 min after ovalbumin or saline inhalation. The exercise capacity, airway responsiveness, lung morphology, the morphological changes and endocrine function of AMCCs were measured in both groups of rats after exercise training for 6 weeks. Either low-intensity or moderate-intensity exercise mitigated EIB and increased exercise capacity in ovalbumin-sensitized (asthmatic) rats. Low-intensity aerobic exercise reduced the vacuolar degeneration degrees, lipid contents, neuronal peripherin and neurofilament-68 expression, demolished neurites, but increased the chromaffin granule density, endocrine chromogranin A and phenylethanolamine N-methyltransferase expression in the adrenal medullary tissues, accompanied by increased levels of circulating epinephrine and corticosterone, but decreased nerve growth factor in asthmatic rats. Finally, low-intensity aerobic exercise significantly reduced the relative levels of phosphorylated extracellular signalregulated kinase and phosphorylated cAMP responsive element-binding protein and the relative mRNA expression levels of downstream molecules, including c-FOS and c-JUN in the adrenal medullary of asthmatic rats. We suggest that low-intensity aerobic exercise improves the endocrine dysfunction of AMCCs and mitigates EIB.

show abstract

“…Дисбаланс в экспрессии этих рецепторов в гладкой мускулатуре дыхательных путей может играть роль в патогенезе БА. Отмечено, что у больных БА сни жен уровень эндогенного адреналина, чем объясня ется его высокая эффективность, связанная с введе нием экзогенного адреналина при БА [23]. Кроме того, при активации β2 адренорецепторов ингибиру ется функция различных воспалительных клеток, участвующих в патогенезе БА, в т. ч. тормозится IgE опосредованное высвобождение гистамина [24], овальбумин индуцированная инфильтрация эози нофилами дыхательных путей и адгезия эозинофи лов на фибробластах легких, продукция IL 4 и IL 13 Т клетками человека [25].…”

Section: обзорыunclassified

Comorbidity of asthma and diabetes: synergism or antagonism?

Ivanov¹,

Сорокина²,

Минеев³

et al. 2014

Pulʹmonologiâ (Mosk.)

View full text Add to dashboard Cite

SummaryAsthma is a chronic inflammatory disease with the growing prevalence over the last 50 years. Some endocrine disorders including diabetes mellitus and obesity were identified as important factors affecting asthma course. Currently, controversial data on probable role of Th2 imbalance for patho genesis of type 2 diabetes mellitus have been published. Diabetes and asthma could have similar pathogenic mechanisms which are poorly under stood. There is a lack of Russian publications on this problem. Comorbidity of asthma and diabetes could be synergic or antagonistic. Conclusion. Further investigations of comorbidity of asthma and diabetes is necessary.

show abstract

The Effect of Unilateral Adrenalectomy on Transformation of Adrenal Medullary Chromaffin Cells In Vivo: A Potential Mechanism of Asthma Pathogenesis

Cited by 10 publications

References 23 publications

High-intensity training induces EIB in rats through neuron transdifferentiation of adrenal medulla chromaffin cells

High-intensity training induces EIB in rats through neuron transdifferentiation of adrenal medulla chromaffin cells

Low-Intensity Aerobic Exercise Mitigates Exercise-Induced Bronchoconstriction by Improving the Function of Adrenal Medullary Chromaffin Cells in Asthmatic Rats

Comorbidity of asthma and diabetes: synergism or antagonism?

Contact Info

Product

Resources

About