The effect of ultrasound on the growth and viability of microalgae cells

Joyce, Eadaoin; King, Patrick M.; Mason, Timothy J.

doi:10.1007/s10811-013-0202-5

Cited by 27 publications

(10 citation statements)

References 20 publications

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“…The method employed for cell disruption determines the quality and yields of the intracellular metabolites. The efficiencies of the cell wall disruption methods depend on cell wall strength (Joyce et al 2014). The composition and structure of the microalgal cell wall varies among strains.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Parachlorella kessleri cell disruption evaluated with the use of laser light scattering methods

et al. 2018

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The main objective of this study is to demonstrate the possibilities of using laser light scattering methods, dynamic light scattering and laser Doppler electrophoresis, as suitable methods in investigations of algal production biosystems and biotechnology. This paper highlights the innovative use of the dynamic light scattering (DLS) methods for monitoring the destruction of Parachlorella kessleri cells. Additionally, these results indicate electrophoretic mobility as a new parameter to investigate the effectiveness of cell disruption prior to extraction conducted to optimise the biotechnological processes of recovery of microalgal intracellular metabolites. The efficacy of P. kessleri cell disintegration by ultrasound was determined by measurements of the number of cells with the algal cell reduction (CR ns), relative mean hydrodynamic diameter (Rd t) and electrophoretic mobility after applying different lengths of ultrasound exposure to a cell suspension. It was found that stationary-phase cells were the most resistant to the ultrasound treatment, especially at low values of the optical density. Both the relative hydrodynamic diameter and the electrophoretic mobility of cells were correlated statistically significantly with the time of sonication (t) and the algal cell reduction. The relationships allowed estimation of the sonication time needed for total cell disruption.

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Section: Introductionmentioning

confidence: 99%

Effectiveness of Parachlorella kessleri cell disruption evaluated with the use of laser light scattering methods

et al. 2018

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“…Neto et al [71] reported that for lipid extraction from diatom microalgae, sonication greatly impacted the cell disruption and improved the oil recovery. Joyce et al [80] reported that suspensions of Nannochloropsis oculata was unaffected by the ultrasound treatment as a result of varying microalgae cell wall thickness but Dunaliella salina and Chlorella concordia resulted in complete cell disruption with 4 min and 16 min sonication treatments, respectively. Nowotarski et al [76] reported that sonication of Dunnaliella salina was effectively disrupted at low concentrations while Nannochloropsis oculata was much more resistant to sonication even at long sonication exposures.…”

Section: Microalgae Typementioning

confidence: 99%

“…The use of high-intensity sonication can result in pressurize/heat that is damaging to the cells or tissue [67]. This technique can be species specific since it has been noted to be ineffective in the destruction of diatom species with thick cellular coating [76,78,80].…”

Section: Advantages and Disadvantagesmentioning

confidence: 99%

Microalgae Oil Extraction Pre-treatment Methods: Critical Review and Comparative Analysis

hattab¹,

Ghaly²

2015

J Fundam Renewable Energy Appl

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Microalgae biomass can be used to produce numerous value added products such as biodiesel, bioethanol, biogas, biohydrogen, fish feed, animal feed, human food supplements and skin care products. Production of value added products from microalgae biomass requires the growth and recovery of the algae biomass, extraction and downstream processing of the desired product. One of the major obstacles for using microalgae biomass on an industrial-scale, for the production of biodiesel, is the high processing costs. Increasing the lipid recovery efficiency from the microalgae biomass would result in greater product yields (biodiesel). Thus, the aim of this study was to review the current methods used for microalgae pre-treatment and perform a comparative analysis in order to determine the most economically efficient method for large scale use. The effectiveness of the pre-treatment methods investigated was evaluated based on: (a) cell wall disruption efficiency, (b) cost, (c) toxicity (d) suitability for large scale use, (e) time, (f) reusability and (g) maintenance. Different treatment methods included mechanical techniques (shaking vessel and agitated bead mills and horn and bath sonication), thermal methods (steam explosion, freeze drying and autoclave), electromagnetic radiation (microwave) and biological treatments (enzymatic). The results indicated that of the 9 microalgae methods investigated a mechanical, thermal and electromagnetic radiation techniques were suitable. These methods were bath sonication (81), steam explosion (93) and microwave radiation (87). Microwave assisted microalgae pre-treatment technique is rapid, effective in cell wall disruption, non-toxic, can be used for large volumes and the medium maybe reused, but it does however suffer from high maintenance costs. Bath sonication technique is effective in the degradation of cell wall, nontoxic, rapid technique with minimal maintenance required, but suffers from high costs and difficulty in scale up for industrial use. Steam explosion pre-treatment is effective in degrading microalgae cell wall, releasing intracellular components, rapid, reusable, relatively low in costs, environmentally friendly and reusable, but is species specific. Overall, the negative aspects of these three techniques are outweighed by their effectiveness, rapidness and relatively low costs when compared to other pre-treatment techniques. Other mechanical extraction methods suffer from high operational costs, lengthy treatment times, high maintenance costs and the scale up difficulty. Freeze drying and autoclave techniques were deemed unsuitable microalgae pre-treatment techniques because of the high costs, scale up difficulty and long processing times associated. Biological pre-treatment technique were deemed unsuitable as a result of high costs associated with purchasing of enzymes, difficulty in recovery/separation after treatment, long treatment time, and high maintenance required for high efficiency.

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“…Previous studies already highlighted the potential of ultrasound to disrupt algae cells, although the efficacy of the applied treatments (20 kHz, power 500 W) depended on the type of microalgae (Ranjan, Patil, & Moholkar, 2010). Recently, Joyce, King, and Mason (2014) reported that the efficacy of a 20 kHz US treatment to disrupt microalgae cells was specie dependent. Cell disruption of Dunaliella salina and Chlamydomonas concordia was observed after 4 and 16 min, respectively, while under the same conditions, there was no visible disruption for Nannochloropsis oculata (Joyce et al, 2014).…”

Section: Suitability Of Chlorine and Us To Disinfect Irrigation Watermentioning

confidence: 99%

“…Recently, Joyce, King, and Mason (2014) reported that the efficacy of a 20 kHz US treatment to disrupt microalgae cells was specie dependent. Cell disruption of Dunaliella salina and Chlamydomonas concordia was observed after 4 and 16 min, respectively, while under the same conditions, there was no visible disruption for Nannochloropsis oculata (Joyce et al, 2014). Based on these results, the types of algae present in the tested irrigation water seem to be relatively susceptible to the US treatments tested in this study.…”

Section: Suitability Of Chlorine and Us To Disinfect Irrigation Watermentioning

confidence: 99%

Ultrasound treatments improve the microbiological quality of water reservoirs used for the irrigation of fresh produce

Villanueva¹,

Luna

Gil

et al. 2015

Food Research International

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The effect of ultrasound on the growth and viability of microalgae cells

Cited by 27 publications

References 20 publications

Effectiveness of Parachlorella kessleri cell disruption evaluated with the use of laser light scattering methods

Effectiveness of Parachlorella kessleri cell disruption evaluated with the use of laser light scattering methods

Microalgae Oil Extraction Pre-treatment Methods: Critical Review and Comparative Analysis

Ultrasound treatments improve the microbiological quality of water reservoirs used for the irrigation of fresh produce

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