The effect of U-74389G on pancreas ischemia–reperfusion injury in a swine model

Chrysikos, Dimosthenis; Sergentanis, Theodoros N.; Zagouri, Flora; Ψαλτοπούλου, Θεοδώρα; Flessas, Ioannis; Agrogiannis, George; Alexakis, N.; Bramis, Ioannis; Patsouri, Evgenia E.; Patsouris, Efstratios; Korontzi, M.; Κατσαρού, Αλεξάνδρα; Zografos, George; Papalois, Αpostolos

doi:10.1016/j.jss.2013.11.1082

Cited by 10 publications

(10 citation statements)

References 33 publications

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“…153190-29-5, for experimental use). This lazaroid dose has also been used in previous experimental studies from the same laboratory using U-74389G (6)(7)(8); this dose has also been used in various other experimental models (6)(7)(8)23) and appears to be beneficial. The ascorbic acid dose (VITAMIN-C-LOGES Optipharm Co, 1 ampule 100 mg/ml) was also 10 mg/kg body weight.…”

Section: Methodsmentioning

confidence: 99%

“…Considering that the lazaroids' mechanism of action is free radical scavenging, we hypothesized that MDA levels could accurately estimate lazaroid's effect in experimental studies, such as those using RIRI models. Moreover, in experimental studies of IRI, U-74389G decreased TNF-α levels (6)(7)(8).…”

mentioning

confidence: 90%

“…Free oxygen radicals are mainly responsible for RIRI. Studies (4,5) have reported that ischemia and reperfusion generate a systemic inflammatory cascade in various organs (e.g., pancreas, liver, and intestines) by free oxygen radical and cytokine (6)(7)(8) production.…”

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confidence: 99%

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The Effects of Ascorbic Acid and U-74389G on Renal Ischemia-Reperfusion Injury in a Rat Model

Zografos

Chrysikos

Pittaras

et al. 2020

In Vivo

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Background/Aim: U-74389G and ascorbic acid protect the cells from oxidation. This study aimed to depict their role in ischemia-reperfusion injury in a renal rat model. Materials and Methods: Sixty Wistars rats were randomized into six groups of 10 animals each. Group A Ischemia 30 min, reperfusion 60 min; Group B Ischemia 30 min, reperfusion 120 min; Group C Ischemia 30 min, ascorbic acid administration, reperfusion 60 min; Group D Ischemia 30 min, ascorbic acid administration, reperfusion 120 min; Group E Ischemia 30 min, U-74389G administration, reperfusion 60 min; Group F Ischemia 30 min, U-74389G administration, reperfusion 120 min. We then collected tissue and blood samples. Results: Histology and the significantly decreased malondialdehyde and tumor necrosis factor-α levels indicated that ascorbic acid was superior to U-74389G, at pre-defined time intervals. Conclusion: Ascorbic acid and U-74389G ameliorated renal damage induced by ischemia-reperfusion injury, suggesting a therapeutic effect.

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Section: Methodsmentioning

confidence: 99%

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confidence: 90%

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The Effects of Ascorbic Acid and U-74389G on Renal Ischemia-Reperfusion Injury in a Rat Model

Zografos

Chrysikos

Pittaras

et al. 2020

In Vivo

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“…The appropriate dose of lazaroids is a topic under investigation. The dose of 10 mg/kg appears to be the most effective in various studies with liver I/R injury (13)(14)(15). The mechanism of action of U-74389G in liver or intestinal ischemia in swine is based on the inhibition of lipid peroxidation (as evidenced by the attenuation of MDA), the reduction of TNF-α tissue levels and the improvement of intestinal and liver histopathological parameters The reduction in the serum levels of TNF-α may also contribute to the reduction of leukocyte infiltration (36).…”

Section: First Set Of Experiments Second Set Of Experiments ---------mentioning

confidence: 99%

“…The lazaroid U-74389G has been studied in various ischemia-reperfusion (I/R) injury models in vitro and in vivo, including rat liver lyosomes subjected to exogenously generated oxygen free radicals in vitro (8,11), renal I/R injury (12), pancreatic I/R injury in pigs (13,14), intestinal I/R injury in rats (15), orthotopic heart transplantation in mongrel dogs (16), as well as in canine liver preservation (17). The results indicated various mechanisms of action for the lazaroid U-74389G, including inhibition of lipid peroxidation, stabilization of the cellular membrane by incorporation into the lipid bilayer, suppression of pro-inflammatory gene expression by nuclear factor (NF)-κB inhibition, prevention of polymorphonuclear cell infiltration, scavenging of lipid peroxyl radicals and reduction of tumor necrosis factor-α (TNF-α) release (6,(10)(11)(12)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

Lazaroid U‑74389G in liver ischemia‑reperfusion injury: A swine model

et al. 2019

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Reactive oxygen species have a key role in liver ischemia-reperfusion (I/R) injury. In the present study, the effect of the anti-oxidant compound lazaroid U-74389G in preventing liver I/R injury was investigated in a swine model. Ischemia was produced by portal vein occlusion. Two sets of experiments were performed, each with two groups (n=7 per group). In the first group, the potential protective effect of an intracaval injection of U-74389G after a 30-min ischemia, followed by a 60-min reperfusion period was assessed (biopsies at 0, 15, 30 and 90 min experimental time). In the second set, the effect of intracaval U-74389G injection after 30 min of ischemia, followed by a longer reperfusion period of 120 min was determined (biopsies at 0, 15, 30 and 150 min experimental time). Liver malondialdehyde, hepatocyte vacuolation-degeneration, venous congestion, inflammatory cell infiltration, sinus congestion-dilation and Chiu score of intestinal damage were determined at up to 150 min of reperfusion. In the second set of experiments, the Chiu score of intestinal damage was improved by the administration of U-74389G (3.17±0.40 vs. 4.33±0.21; P=0.030). However, in the two sets of experiments, the liver inflammatory reaction was more pronounced in the U-74389G groups (P=0.017 for the first set, P= 0.021 for the second set). No significant effect of U-74389G on any other parameters was detected. In conclusion, intestinal damage due to portal venous congestion

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