The effect of type II collagen coating of chitosan fibrous scaffolds on mesenchymal stem cell adhesion and chondrogenesis

Ragetly, Guillaume; Griffon, Dominique J.; Chung, Yong Sik

doi:10.1016/j.actbio.2010.05.016

Cited by 75 publications

(50 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that collagen Type II hydrogels resulted in the more prominent chondrogenic differentiation of MSC, 80 compared to alginate or collagen Type I. Further, collagen Type II coatings on alginate microbeads 81 or chitosan fibrous scaffolds 82 can enhance MSC proliferation and chondrogenesis. It is likely that a higher MSC concentration and a matrix formulation more conducive to chondrogenesis would be required to create microbeads for cartilage tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Uncultured Marrow Mononuclear Cells and Culture-Expanded Mesenchymal Stem Cells in 3D Collagen-Chitosan Microbeads for Orthopedic Tissue Engineering

Wise

Alford

Goldstein

et al. 2014

Tissue Engineering Part A

View full text Add to dashboard Cite

Stem cell-based therapies have shown promise in enhancing repair of bone and cartilage. Marrow-derived mesenchymal stem cells (MSC) are typically expanded in vitro to increase cell number, but this process is lengthy, costly, and there is a risk of contamination and altered cellular properties. Potential advantages of using fresh uncultured bone marrow mononuclear cells (BMMC) include heterotypic cell and paracrine interactions between MSC and other marrow-derived cells including hematopoietic, endothelial, and other progenitor cells. In the present study, we compared the osteogenic and chondrogenic potential of freshly isolated BMMC to that of cultured-expanded MSC, when encapsulated in three-dimensional (3D) collagen-chitosan microbeads. The effect of low and high oxygen tension on cell function and differentiation into orthopedic lineages was also examined. Freshly isolated rat BMMC (25 · 10 6 cells/mL, containing an estimated 5 · 10 4 MSC/mL) or purified and culture-expanded rat bone marrow-derived MSC (2 · 10 5 cells/mL) were added to a 65-35 wt% collagenchitosan hydrogel mixture and fabricated into 3D microbeads by emulsification and thermal gelation. Microbeads were cultured in control MSC growth media in either 20% O 2 (normoxia) or 5% O 2 (hypoxia) for an initial 3 days, and then in control, osteogenic, or chondrogenic media for an additional 21 days. Microbead preparations were evaluated for viability, total DNA content, calcium deposition, and osteocalcin and sulfated glycosaminoglycan expression, and they were examined histologically. Hypoxia enhanced initial progenitor cell survival in fresh BMMC-microbeads, but it did not enhance osteogenic potential. Fresh uncultured BMMCmicrobeads showed a similar degree of osteogenesis as culture-expanded MSC-microbeads, even though they initially contained only 1/10th the number of MSC. Chondrogenic differentiation was not strongly supported in any of the microbead formulations. This study demonstrates the microbead-based approach to culturing and delivering cells for tissue regeneration, and suggests that fresh BMMC may be an alternative to using cultureexpanded MSC for bone tissue engineering.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of Uncultured Marrow Mononuclear Cells and Culture-Expanded Mesenchymal Stem Cells in 3D Collagen-Chitosan Microbeads for Orthopedic Tissue Engineering

Wise

Alford

Goldstein

et al. 2014

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…Porous chitosan scaffolds with thickness between 60 and 80 μm produced by lyophilization proved to be appropriate for the in vitro culture of dermal fibroblasts, showing also to be potentially suitable for the use in skin tissue engineering (Ma et al 2001). Chitosan-alginate membranes designed for treating skin lesions had thicknesses between 23.5 and 80 μm Ragetly et al 2010). Films of chitosan complexed with xanthan gum of analytical grade at C:X mass ratios of 1:1 and 1.2:0.8 had mean thickness varying from 100 to 200 μm (Bellini et al 2012), similar to membranes prepared by combining C-X Keltrol in different conditions from those used in the present work, being, therefore, in the same range of the values reported herein.…”

Section: Physico-chemical Characterization Of the Filmsmentioning

confidence: 99%

“…Its production process is relatively simple and one of its most important characteristics regarding biological applications is its degree of deacetylation. This polymer is soluble in aqueous acidic solutions, resulting in structures with different dimensions and geometric configurations, such as films, particles, fibers, and gels (Santos et al 2003;Ragetly et al 2010). The polycationic nature of chitosan enables its association with polymers with negative charges such as alginate (Rodrigues et al 2008;Bueno and Moraes 2011), dextran derivatives (Fukuda et al 1978), polyesters (Silva et al 2010), silk fibers (She et al 2008), gelatin (Liu et al 2004;Yin et al 2005), and xanthan gum (Eftaiha et al 2009;Bellini et al 2012;Veiga and Moraes 2012), resulting in stable polyelectrolyte complexes.…”

Section: Introductionmentioning

confidence: 99%

Properties of films obtained from biopolymers of different origins for skin lesions therapy

Bellini

Neto

Moraes

2015

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…As collagen and chitosan are amongst the most abundant polymers in nature, each has been used in the design of drug delivery systems and for the formation of scaffolds in tissue engineering applications (Lee et al, 2008). Some studies have reported beneficial uses of collagen/chitosan scaffolds in the culturing of several cell types, including endothelial cells (Mori et al, 1998), fibroblasts (Mori et al, 1997), mesenchymal stem cells (Ragetly et al, 2010), nerve cells (Li et al, 2014) and chondrocytes (Lahiji et al, 2000). These scaffolds also have been used for tissue/organ engineering, including skin, bones, blood vessels and nerve conduits.…”

Section: Introductionmentioning

confidence: 99%

Effects of 3-dimensional culture conditions (collagen-chitosan nano-scaffolds) on maturation of dendritic cells and their capacity to interact with T-lymphocytes

Daneshmandi

Dibazar

Fateh

2015

Journal of Immunotoxicology

View full text Add to dashboard Cite

In the body, there is a natural three-dimensional (3D) microenvironment in which immune cells, including dendritic cells (DC), play their functions. This study evaluated the impact of using collagen-chitosan 3D nano-scaffolds in comparisons to routine 2D culture plates on DC phenotype and functions. Bone marrow-derived DC were cultured on scaffolds and plates and then stimulated with lipopolysaccharide (LPS) or chitosan-based nanoparticles (NP) for 24 h. Thereafter, DC viability, expression of maturation markers and levels of cytokines secretion were evaluated. In another set of studies, the DC were co-cultured with allogenic T-lymphocytes in both the 2D and 3D systems and effects on DC-induction of T-lymphocyte proliferation and cytokine release were analyzed. The results indicated that CD40, CD86 and MHC II marker expression and interleukin (IL)-12, IL-6 and tumor necrosis factor (TNF)-secretion by DC were enhanced in 3D cultures in comparison to by cells maintained in the 2D states. The data also showed that DNA/chitosan NP activated DC more than LPS in the 3D system. T-Lymphocyte proliferation was induced to a greater extent by DNA/NP-treated DC when both cell types were maintained on the scaffolds. Interestingly, while DC induction of T-lymphocyte interferon (IFN)-and IL-4 release was enhanced in the 3D system (relative to controls), there was a suppression of transforming growth factor (TGF)-production; effects on IL-10 secretion were variable. The results here suggested that collagen-chitosan scaffolds could provide a proinflammatory and activator environment to perform studies to analyze effects of exogenous agents on the induction of DC maturation, NP uptake and/or cytokines release, as well as for the ability of these cells to potentially interact with other immune system cells in vitro.

show abstract

The effect of type II collagen coating of chitosan fibrous scaffolds on mesenchymal stem cell adhesion and chondrogenesis

Cited by 75 publications

References 71 publications

Comparison of Uncultured Marrow Mononuclear Cells and Culture-Expanded Mesenchymal Stem Cells in 3D Collagen-Chitosan Microbeads for Orthopedic Tissue Engineering

Comparison of Uncultured Marrow Mononuclear Cells and Culture-Expanded Mesenchymal Stem Cells in 3D Collagen-Chitosan Microbeads for Orthopedic Tissue Engineering

Properties of films obtained from biopolymers of different origins for skin lesions therapy

Effects of 3-dimensional culture conditions (collagen-chitosan nano-scaffolds) on maturation of dendritic cells and their capacity to interact with T-lymphocytes

Contact Info

Product

Resources

About