The effect of turpentine-induced inflammation on rat liver glycosyltransferases and golgi complex ultrastructure

Changes in the morphology and phosphatase cytochemistry of the Golgi region of hepatocytes during the acute phase response to inflammation

“…The present study showed that these biochemical events coincided with the appearance in the hepatocyte Golgi region of secretory granules with a n altered content. The Golgi stacks which produced these modified secretory granules were enlarged as also documented earlier (Turchen et al, 1977;Lombart et al, 1980), but retained their organization into four morphologically and biochemically distinct compartments. The present study in addition showed that two out of the four Golgi compartments were particularly affected by the turpentine inflammation: the two saccules comprising the mid-compartment and the TGN.…”

“…These plasma proteins, most of which are glycoproteins (Gordon and Koj, 1985;Jamieson et al, 1987), are usually referred to as the acute phase reactants. The process was first shown by us (Turchen et al, 1977) to result in an increase in the size of the Golgi apparatus; others have since quantified this increase morphometrically and found it to be accompanied by an elevated ratio of total Golgi protein to total cell protein (Lombart et al, 1980). The present study aims to extend our earlier work by monitoring the distribution and size of individual compartments of the hepatocyte Golgi from turpentine-inf lamed animals, by cytochemically localizing nicotinamide adenine dinucleotide phosphatase (NADPase), thiamine pyrophosphatase (TPPase), and cytidine monophosphatase (CMPase) which previously have been shown to be Golgi andlor lysosomal marker enzymes in hepatocytes (Novikoff and Yam, 1978;Bennett and OShaughnessy, 1981;Roth et al, 1985;Jost-Vu et al, 1986;Smith et al, 1986;Taatjes and Roth, 1986;Brightmann et al, 1992).…”

Activity and secretion of sialyltransferase in primary monolayer cultures of rat hepatocytes cultured with and without dexamethasone

“…It can be assumed that the secretion of the soluble sialyltransferase activity into culture media or into serum serve some physiological purpose. Jamieson and co-workers (4,5) and others (6,(27)(28)(29) have shown that glycosyltransferase activities in rat liver are increased following turpentine-induced inflammation. Some of these studies also showed that sialyltransferase, but not galactosyltransferase, is increased in serum owing to inflammation.…”

Studies on carbohydrate moieties of glycoproteins in established adjuvant induced arthritis

“…Trnasvska et al [4] demonstrated that alteration in the metabolic turnover of collagen manifested by an increase in the breakdown of newly formed collagen in acute phase and by retardation in the conversion of soluble to insoluble collagen in chronic phase of the arthritic syndrome. Lambert et al [7] reported that the activity ofglycosyltransferase and the rate of transfer of N-acetylglucosamine, galactose and sialic acid to an appropriate sugardepleted acceptor glycopeptide were increased in the process of inflammatory disease. During the process of the inflammation, these hydrolytic enzymes were released from invading macrophages and neutrophils and from tissue cells such as synoviocytes and chondrocytes.…”