The Effect of Tuning Cold Plasma Composition on Glioblastoma Cell Viability

Abstract

Previous research in cold atmospheric plasma (CAP) and cancer cell interaction has repeatedly proven that the cold plasma induced cell death. It is postulated that the reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a major role in the CAP cancer therapy. In this paper, we seek to determine a mechanism of CAP therapy on glioblastoma cells (U87) through an understanding of the composition of the plasma, including treatment time, voltage, flow-rate and plasma-gas composition. In order to determine the threshold of plasma treatment on U87, normal human astrocytes (E6/E7) were used as the comparison cell line. Our data showed that the 30 sec plasma treatment caused 3-fold cell death in the U87 cells compared to the E6/E7 cells. All the other compositions of cold plasma were performed based on this result: plasma treatment time was maintained at 30 s per well while other plasma characteristics such as voltage, flow rate of source gas, and composition of source gas were changed one at a time to vary the intensity of the reactive species composition in the plasma jet, which may finally have various effect on cells reflected by cell viability. We defined a term “plasma dosage” to summarize the relationship of all the characteristics and cell viability.

Mechanisms of Inactivation by High-Voltage Atmospheric Cold Plasma Differ for Escherichia coli and Staphylococcus aureus

“…A similar correlation of ROS generation with time was reported by other researchers using plasma jet treatment. Intracellular ROS increased over 5 min of treatment by air plasma from a jet (36), with a similar trend reported for the generation of RNS (37). Plasma treatment time determines the input energy during discharge.…”

Computational Fluid Dynamics Analysis of Cold Plasma Plume Mixing with Blood Using Level Set Method Coupled with Heat Transfer

“…Cold atmospheric plasmas have shown significant potentials in various biomedical applications such as wound healing [4,5], tooth bleaching [6], cancer therapy [7][8][9] and sterilization of infected tissues [10]. In recent years, cold atmospheric plasmas were explored for inactivating different microorganisms.…”

Atmospheric cold plasma interactions with modified atmosphere packaging inducer gases for safe food preservation

“…The in-package treatment design 86 employed in this study has proven efficacy for decontamination of tomatoes, strawberries and 87 fresh meat slices (Han, et al, 2016;N. Misra, Patil, et al, 2014;Ziuzina, Patil, Cullen, 88 Keener, & Bourke, 2014), demonstrating potential for adoption for a range other fresh foods 89 where safe shelf-life extension is required to meet emerging sustainability and innovation with process and system parameters and the reactive species generated (Cheng, et al, 2014;94 Han, et al, 2014). Besides gas composition, post treatment storage time is a critical parameter 95 influencing ACP inactivation efficacy.…”

“…Similarly, longer 228 treatment times and higher oxygen content lead to more ROS generation (Cheng, et al, 2014;229 Han, et al, 2014). The underlying mechanism was further observed as the generation of …”