The effect of tumor necrosis factor α (TNFα), interleukin 1β (IL1β) and interleukin 6 (IL6) on endometrial PGF2α synthesis, metabolism and release in early-pregnant pigs

The expression of mRNAs for opioid precursors in endometrium of cyclic and early pregnant pigs; effects of IL-1β, IL-6 and TNFα

“…Subsequently, the tissue was sliced into 200-210 mg explants, washed twice with PBS and preincubated in 2 ml of Medium 199 (Sigma-Aldrich, Germany) with the addition of 0.1% bovine serum albumin fraction V (ICN, USA) and 20 µg of gentamycin (Sigma-Aldrich, Germany) for 18 h at 37°C under an atmosphere of 95% O 2 and 5% CO 2 . Then the medium was replaced and explants were incubated for 12 h without or with addition of IL-1β, IL-6 or TNFα in amounts (10 ng · ml -1 ) previously used by Franczak et al (2012). All cytokines have been purchased from Biomol, GmbH, Germany.…”

The expression of mRNAs for opioid receptors in endometrium of cyclic and early pregnant pigs; <i>in vitro</i> effects of IL-1β, IL-6 and TNFα

“…All cytokines have been purchased from Biomol GmbH (Hamburg, Germany). The concentrations of studied factors were selected on the basis of the previous studies by Franczak et al (2012). After incubation, the tissue explants were recovered, washed with PBS and stored at -80°C for further analysis.…”

“…In rats, the κ opioid receptor mRNA level was reduced by IL-6 in primary Sertoli cells (Jenab and Morris, 2000). It is known that cytokines may affect different processes in the uterus, such as secretory activity, adaptation to the early pregnancy or immunomodulation (Dimitriadis et al, 2005;Waclawik, 2011;Ziecik et al, 2011;Franczak et al, 2012Franczak et al, , 2013Okrasa et al, 2014), however their effect on the expression of genes encoding opioid receptors in this organ has not been tested yet.…”

Impairment of the Interleukin System in Equine Endometrium During the Course of Endometrosis1

“…In fact, IL may be responsible for disturbances in physiological processes in endometrium, contributing to impairment of CL maintenance, cell proliferation, and angiogenesis during the estrous cycle, and to early embryo loss. Studies on the association of IL with PGs, in species other than the horse, showed that they may be among the most important components of physiological changes in estrous cycle regulation [12][13][14][15][16]. Furthermore, in the peri-implantation period, ILs participate in conducting several processes, including differentiation of endometrial cells and vascular endothelial cell changes, that accompany implantation and subsequent placental development.…”

“…Interleukin (IL) 1a and IL1b or IL6 are known to take part in regulation of the estrous cycle by modulation of PG synthesis in species other than the horse [12][13][14][15][16]. In particular, IL1a seems to be a potent luteotropic factor supporting early pregnancy development through stimulation of P 4 and PGE 2 production in the cow [14].…”