The effect of TSPP-mediated photodynamic therapy and Parecoxib in experimental tumours

Popescu, Tiberiu; Nenu, Iuliana; Aldea, Mihaela; Olteanu, Diana; Gheban, Dan; Tatomir, Corina; Bolfă, Pompei; Muresan, Adriana; Ion, Rodica‐Mariana; Filip, Gabriela Adriana

doi:10.1016/j.lfs.2014.09.022

Cited by 9 publications

(5 citation statements)

References 36 publications

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“…In addition, administration of parecoxib after 5,10,15,20-tetra-sulphonato-phenyl-porphyrin-mediated photodynamic therapy showed promising antitumor effects, leading to an increase in oxidative and nitrosative stress and apoptosis/necrosis rate in tumor tissue. 17 In spite of these findings, our studies demonstrated that the apoptosis effect did not appear during the parecoxib treatment of DLD-1 cells. Even the cell viability maintained upper 99% on 10 μM of parecoxib treatment after 72 h. Except for the apoptosis effect, parecoxib treatment apparently indicated a novel anticancer mechanism in DLD-1 cells.…”

Section: Anti-migration Effect and Cox-2 Inhibition Of Parecoxib In S...contrasting

confidence: 71%

“…In xenograft experiments, parecoxib also enhanced the tumor response of HCT116 to radiation. In addition, administration of parecoxib after 5,10,15,20‐tetra‐sulphonato‐phenyl‐porphyrin‐mediated photodynamic therapy showed promising antitumor effects, leading to an increase in oxidative and nitrosative stress and apoptosis/necrosis rate in tumor tissue 17 . In spite of these findings, our studies demonstrated that the apoptosis effect did not appear during the parecoxib treatment of DLD‐1 cells.…”

Section: Discussioncontrasting

confidence: 55%

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Parecoxib expresses anti‐metastasis effect through inhibition of epithelial‐mesenchymal transition and the Wnt/β‐catenin signaling pathway in human colon cancer DLD‐1 cell line

Wong

Chang

et al. 2022

Environmental Toxicology

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Colorectal cancer is the third leading cause of cancer death in Taiwan. Current treatments involve combination of surgical resection, radiation, and chemotherapy. These treatments have demonstrated to increased five‐year survival of a patient with colorectal cancer. However, metastasis is a major capability of cancer cells that causes poor prognosis, recurrence, and even death. Epidemiological and clinical studies have suggested the use of non‐steroidal anti‐inflammatory drugs (NSAIDs) as an effective class of compounds to prevent colon cancer. Parecoxib is an NSAID and the only parenterally administered selective cyclooxygenase (COX)‐2 inhibitor. In this study, we evaluated whether parecoxib inhibits the metastasis of DLD‐1 human colon cancer cells, a COX‐2 null cell line, and the underlying mechanism. Cell migration of the DLD‐1 cells was significantly inhibited by parecoxib treatment as shown by the Transwell migration assay. This enhanced anti‐migration effect was correlated with the attenuated phosphorylation of Akt, expression of vimentin (a mesenchymal marker), and β‐catenin, and corresponded with the upregulated GSK3β and E‐cadherin (an epithelial marker). These findings suggested that parecoxib could inhibit the epithelial‐mesenchymal transition (EMT) and metastasis in human colon cancer cells by downregulating β‐catenin. Thus, parecoxib could provide a novel prospective strategy for a combination treatment with chemotherapeutic drugs against metastasis of human colon cancer.

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Section: Anti-migration Effect and Cox-2 Inhibition Of Parecoxib In S...contrasting

confidence: 71%

Section: Discussioncontrasting

confidence: 55%

Parecoxib expresses anti‐metastasis effect through inhibition of epithelial‐mesenchymal transition and the Wnt/β‐catenin signaling pathway in human colon cancer DLD‐1 cell line

Wong

Chang

et al. 2022

Environmental Toxicology

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“…PDT is an ideal oncological approach because induces direct tumor cell photo damage and also targets tumor vasculature and activates the immune response [91, 92]. Despite these effects, there is a limited experience of PDT in melanoma and extensive clinical studies need to be taken into consideration, using selected photosensitizers and standard irradiation protocols and also several combination regimens with chemotherapy or immunotherapy, in order to obtain an established antitumor effect [11].…”

Section: Discussionmentioning

confidence: 99%

Combined regimen of photodynamic therapy mediated by Gallium phthalocyanine chloride and Metformin enhances anti-melanoma efficacy

et al. 2017

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BackgroundMelanoma therapy is challenging, especially in advanced cases, due to multiple developed tumor defense mechanisms. Photodynamic therapy (PDT) might represent an adjuvant treatment, because of its bimodal action: tumor destruction and immune system awakening. In this study, a combination of PDT mediated by a metal substituted phthalocyanine—Gallium phthalocyanine chloride (GaPc) and Metformin was used against melanoma. The study aimed to: (1) find the anti-melanoma efficacy of GaPc-PDT, (2) assess possible beneficial effects of Metformin addition to PDT, (3) uncover some of the mechanisms underlining cell killing and anti-angiogenic effects.MethodsTwo human lightly pigmented melanoma cell lines: WM35 and M1/15 subjected to previous Metformin exposure were treated by GaPc-PDT. Cell viability, death mechanism, cytoskeleton alterations, oxidative damage, were assessed by means of colorimetry, flowcytometry, confocal microscopy, spectrophotometry, ELISA, Western Blotting.ResultsGaPc proved an efficient photosensitizer. Metformin addition enhanced cell killing by mechanisms dependent on the cell line, namely apoptosis in the metastatic M1/15 and necrosis in the radial growth phase, WM35. Cell death mechanism relied on the inhibition of nuclear transcription factor (NF)-κB activation and tumor necrosis factor (TNF)—related apoptosis-inducing ligand (TRAIL) sensitization, leading to TRAIL and TNF-α induced apoptosis. Metformin diminished the anti-angiogenic effect of PDT.ConclusionsMetformin addition to GaPc-PDT increased tumor cell killing through enhanced oxidative damage and induction of proapoptotic mechanisms, but altered PDT anti-angiogenic effects.General significanceCombination of Metformin and PDT might represent a solution to enhance the efficacy, leading to a potential adjuvant role of PDT in melanoma therapy.

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“…In order to quantify the redox imbalance induced by PDT and the impact of CUR and RES on the PDT efficiency, the malondialdehyde (MDA) levels and glutathione reduced/glutathione oxidized ratio (GSH/ GSSG) were assessed. MDA levels were determined using the fluorimetric method with 2-thiobarbituric acid [28] and the results were expressed as nmoles/mg protein [29]. Reduced and oxidized glutathione (GSH and GSSG, respectively) were determined using the fluorimetrical method based on o-phthalaldehyde reactive [30].…”

Section: Oxidative and Nitrosative Stress Markersmentioning

confidence: 99%

The comparative effects of Resveratrol and Curcumin in combination with photodynamic therapy

Laszló

Popescu

et al. 2022

Medicine and Pharmacy Reports

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Background and aim. Photodynamic therapy, PDT, is a promising option among the local treatments with oncolytic potential. Although the basic principle is simple, its intricate mechanisms allow for a broad range of optimization methods. The purpose of this study was to assess the effects of Resveratrol and Curcumin as adjuvants of PDT on experimental tumors. Methods. Sixty-six Wistar male rats were divided into 11 groups: control, Curcumin (CUR), Resveratrol (RES) alone or followed by irradiation (IR) (CUR+IR and RES+IR, respectively), 5,10,15,20-tetra-sulphonato-phenyl-porphyrin (TSPP), TSPP+IR (PDT), and CUR or RES administered prior to or after PDT (CUR+TSPP+IR, RES+TSPP+IR, TSPP+IR+CUR, TSPP+IR+RES). Results. Both CUR and RES significantly decreased lipid peroxidation, while RES also showed an increase in glutathione (GSH) levels, especially when it was administered before PDT (p<0.01). Both antioxidants decreased cyclooxygenase (COX)2 expression, to a minimum when they administered prior to PDT (p<0.001 and p<0.01) while nitric oxide synthase (NOS)2 expression diminished in the combined regimen, particularly in RES associated with PDT. CUR and RES induced similar changes in terms of cell death, but CUR seemed to be more efficient on tumor necrosis and showed a higher apoptotic index when was administered after PDT (p<0.001). Conclusion. Both RES and CUR in association with PDT decreased oxidative stress, diminished the COX2 and NOS2 expressions and increased cell death by positively influencing the necrotic rate and apoptotic index, particularly when CUR was administered after PDT. The results show that CUR is a promising class to study in PDT optimization and further invites to exploit its promises.

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The effect of TSPP-mediated photodynamic therapy and Parecoxib in experimental tumours

Cited by 9 publications

References 36 publications

Parecoxib expresses anti‐metastasis effect through inhibition of epithelial‐mesenchymal transition and the Wnt/β‐catenin signaling pathway in human colon cancer DLD‐1 cell line

Parecoxib expresses anti‐metastasis effect through inhibition of epithelial‐mesenchymal transition and the Wnt/β‐catenin signaling pathway in human colon cancer DLD‐1 cell line

Combined regimen of photodynamic therapy mediated by Gallium phthalocyanine chloride and Metformin enhances anti-melanoma efficacy

The comparative effects of Resveratrol and Curcumin in combination with photodynamic therapy

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