The effect of Trp83 mutant on the properties of CopC

Li, Huiqin; Zheng, Xiaoyan; Pang, Erguo; Zhao, Yaqin; Yang, Binsheng

doi:10.1016/j.saa.2007.10.035

Search citation statements

Order By: Relevance

Paper Sections

Select...

The Unfolding Of Copc1

Two-state Model In Thermodynamics1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2009

2014

Publication Types

Select...

Article2

Book1

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When the tryptophan residue 83 is mutated as leucine residue the protein is nearly immeasurable by fluorescence. Meanwhile the mutagenesis make the ability of binding to Cu(II), the thermal stability of apoCopC be decreased [28]. The important role of the tryptophan residue 83 is not only in using as a fluorescence probe but also in keeping the -sandwich hydrophobic structure.…”

Section: The Unfolding Of Copcmentioning

confidence: 99%

The Stability of a Three-State Unfolding Protein

Yang¹

2011

Thermodynamics - Physical Chemistry of Aqueous Systems

View full text Add to dashboard Cite

Section: The Unfolding Of Copcmentioning

confidence: 99%

The Stability of a Three-State Unfolding Protein

Yang¹

2011

Thermodynamics - Physical Chemistry of Aqueous Systems

View full text Add to dashboard Cite

“…19 Once it unfolds, no copper(II) would be bound, or copper(II) would not affect on fluorescence emission of apo-CopC. If there are only native apo-CopC and unfolding states during the denaturation process, the capacity of copper(II) binding would be decided only by the fraction of protein keeping the native fold.…”

Section: Two-state Model In Thermodynamicsmentioning

confidence: 99%

Apo‐CopC and CopC‐Cu(II) Unfolding Characteristics in GuHCl Solution

Zhao

Yang

2009

Chin. J. Chem.

View full text Add to dashboard Cite

The stability of CopC, a copper resistant protein with a Greek β-barrel motif, in GuHCl solution was investigated by fluorescence spectra. Parameter A, characterizing position and shape of the fluorescence spectra, "phase diagram" method of fluorescence, and cupric binding capacity in GuHCl solution of different concentration showed that the denaturation transition of apo-CopC and CopC-Cu(II) might be fitted to a simple two-state model. According to a two-state model, the free energy of stabilization for apo-CopC and CopC-Cu(II), (17.08±0.35) and (23.81±0.45) kJ•mol -1 respectively, was obtained. Copper(II) increased the stability of apo-CopC. The higher thermodynamics stability of CopC-Cu(II) was revealed to originate in both the faster folding and the slower unfolding rates by unfolding kinetics.

show abstract