The Effect of Trisomy-21 (Down’s Syndrome) on Brain Transcription

Lim, Louis; Hall, Christine; Leung, Thomas; Whatley, Stephen A.

doi:10.1007/978-1-4613-2321-1_14

Cited by 2 publications

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References 20 publications

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“…Brain membrane-bound and free polyribosomes contain in common several abundant mRNAs coding for proteins including tubulin, actin, creatine kinase, neuronspecific enolase and a 68 kDa microtubule-associated protein now identified as the heat-shock cognate protein (HSC 70) (Hall & Lim, 1981;Hall et al, 1984;Lim et al, 1984Lim et al, , 1986. These proteins were found to be components of brain synaptic plasma membranes, where the enzymes are involved in ATP generation, as well as having a cytosolic location (Lim et al, 1983;Whatley et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Expression and developmental regulation of two unique mRNAs specific to brain membrane-bound polyribosomes

Hall¹,

Lowndes²,

Leung³

et al. 1987

Biochemical Journal

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Translation in vitro of membrane-bound polyribosomal mRNAs from rat brain has shown several to be developmentally regulated [Hall & Lim (1981) Biochem. J. 196,[327][328][329][330][331][332][333][334][335][336]. Here we describe the isolation and characterization of cDNAs corresponding to two such brain mRNAs. One cDNA (M444) hybridselected a 0.95 kb mRNA directing the synthesis in vitro of a 21 kDa pl-6.3 polypeptide, which was processed in vitro by microsomal membranes. A second cDNA (M1622) hybridized to a 2.2 kb mRNA directing the synthesis of a 55 kDa pl-5.8 polypeptide. Both mRNAs were specific to membrane-bound polyribosomes. Restriction maps of the corresponding genomic DNA sequences are consistent with both being single copy. The two mRNAs were present in astrocytic and neuronal cultures, but not in liver or spleen or in neuroblastoma or glioma cells. The two mRNAs were differently regulated during brain development. In the developing forebrain there was a gradual and sustained increase in M444 mRNA during the first 3 weeks post partum, whereas M 1622 mRNA appeared earlier and showed no further increase after day 10. In the cerebellum the developmental increase in M444 mRNA was biphasic. After a small initial increase there was a decrease in this mRNA at day 10, coincident with high amounts of M1622 mRNA. This was followed by a second, larger, increase in M444 mRNA, when amounts of M1622 mRNA were constant. The contrasting changes in these two mRNAs in the developing cerebellum are of particular interest, since they occur during an intensive period of cell proliferation, migration and altering neural connectivity. As these mRNAs are specific to differentiated neural tissue, they represent useful molecular markers for studying brain differentiation.

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Section: Introductionmentioning

confidence: 99%

Expression and developmental regulation of two unique mRNAs specific to brain membrane-bound polyribosomes

Hall¹,

Lowndes²,

Leung³

et al. 1987

Biochemical Journal

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Localization of a human heat-shock HSP 70 gene sequence to chromosome 6 and detection of two other loci by somatic-cell hybrid and restriction fragment length polymorphism analysis

et al. 1987

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The human 70 kdalton heat-shock protein (HSP 70) is a member of a multigene family which is expressed in response to various physiological stresses including elevated temperatures. Using a cloned genomic HSP 70 DNA sequence we demonstrate by somatic cell hybrid and restriction fragment length polymorphism (RFLP) analyses that there are a minimum of three distinct HSP 70 loci in the human genome, one of which is located on chromosome 6.

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The Effect of Trisomy-21 (Down’s Syndrome) on Brain Transcription

Cited by 2 publications

References 20 publications

Expression and developmental regulation of two unique mRNAs specific to brain membrane-bound polyribosomes

Expression and developmental regulation of two unique mRNAs specific to brain membrane-bound polyribosomes

Localization of a human heat-shock HSP 70 gene sequence to chromosome 6 and detection of two other loci by somatic-cell hybrid and restriction fragment length polymorphism analysis

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