Abstract:The effects of trinitrophenyl (TNP) derivatives of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) on the coupled activities of the tonoplast ATPase in corn roots were investigated. The addition of TNP‐ATP at micromolar concentrations (0.5–0.25 mM) significantly decreased both the ATP hydrolytic activity and the coupled proton pumping activity. The presence of TNP‐ATP resulted in an increased Km for ATP and a reced maximum enzyme velocity. Unlike TNP‐ATP that strongl… Show more
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