The effect of trimethylamine N-oxide on Helicobacter pylori-induced changes of immunoinflammatory genes expression in gastric epithelial cells

Wu, Daoyan; Cao, Mei; Peng, Jingshan; Li, Ningzhe; Yi, Sijun; Song, Lingyun; Wang, Xuege; Zhang, Mao; Zhao, Jian

doi:10.1016/j.intimp.2016.11.032

Cited by 28 publications

(22 citation statements)

References 30 publications

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“…High-fat feeding has been shown to increase the representation of Enterobacteriaceae in the caecal microbiota of obesity-prone Sprague Dawley rats [ 41 ], though this mode of feeding is known to modulate the microbiota of mice independent of obesity [ 42 ]. Non-LPS-associated virulence of Enterobacteriaceae , Vibrio cholerae , and Helicobacter pylori is increased when these bacteria are grown anaerobically or microaerophilically in the presence of as little as 5–10 mM TMAO [ 43 – 46 ], and may be an additional means by which the gut microbiota contributes to CVD and other diseases in which increased representation of Gammaproteobacteria is observed. This warrants attention in future animal studies.…”

Section: Discussionmentioning

confidence: 99%

Metabolic retroconversion of trimethylamine N-oxide and the gut microbiota

et al. 2018

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BackgroundThe dietary methylamines choline, carnitine, and phosphatidylcholine are used by the gut microbiota to produce a range of metabolites, including trimethylamine (TMA). However, little is known about the use of trimethylamine N-oxide (TMAO) by this consortium of microbes.ResultsA feeding study using deuterated TMAO in C57BL6/J mice demonstrated microbial conversion of TMAO to TMA, with uptake of TMA into the bloodstream and its conversion to TMAO. Microbial activity necessary to convert TMAO to TMA was suppressed in antibiotic-treated mice, with deuterated TMAO being taken up directly into the bloodstream. In batch-culture fermentation systems inoculated with human faeces, growth of Enterobacteriaceae was stimulated in the presence of TMAO. Human-derived faecal and caecal bacteria (n = 66 isolates) were screened on solid and liquid media for their ability to use TMAO, with metabolites in spent media analysed by 1H-NMR. As with the in vitro fermentation experiments, TMAO stimulated the growth of Enterobacteriaceae; these bacteria produced most TMA from TMAO. Caecal/small intestinal isolates of Escherichia coli produced more TMA from TMAO than their faecal counterparts. Lactic acid bacteria produced increased amounts of lactate when grown in the presence of TMAO but did not produce large amounts of TMA. Clostridia (sensu stricto), bifidobacteria, and coriobacteria were significantly correlated with TMA production in the mixed fermentation system but did not produce notable quantities of TMA from TMAO in pure culture.ConclusionsReduction of TMAO by the gut microbiota (predominantly Enterobacteriaceae) to TMA followed by host uptake of TMA into the bloodstream from the intestine and its conversion back to TMAO by host hepatic enzymes is an example of metabolic retroconversion. TMAO influences microbial metabolism depending on isolation source and taxon of gut bacterium. Correlation of metabolomic and abundance data from mixed microbiota fermentation systems did not give a true picture of which members of the gut microbiota were responsible for converting TMAO to TMA; only by supplementing the study with pure culture work and additional metabolomics was it possible to increase our understanding of TMAO bioconversions by the human gut microbiota.Electronic supplementary materialThe online version of this article (10.1186/s40168-018-0461-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Metabolic retroconversion of trimethylamine N-oxide and the gut microbiota

et al. 2018

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“…Helicobacter pylori infection is the main risk factor for CAG [ 21 ], which can damage or kill the gastric epithelial cells and alter the gastric acid secretion [ 22 ]. Previous reports have shown that berberine exerts a clearance effect and reduces gastric inflammation during epithelial cell signalling in H. pylori infection [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Exploring the potential pharmacodynamic material basis and pharmacologic mechanism of the Fufang-Xialian-Capsule in chronic atrophic gastritis by network pharmacology approach based on the components absorbed into the blood

Li¹,

Xu²,

Liu³

et al. 2018

R. Soc. open sci.

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In this study, a new network pharmacology approach based on the components absorbed into the blood was used to investigate the pharmacodynamic material basis and the pharmacologic mechanism of the Fufang-Xialian-Capsule (FXL) in treating chronic atrophic gastritis (CAG). Initially, we confirmed the components absorbed into the blood by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Then, the network approach, which was based on the results of components absorbed into the blood, was used to analyse the pharmacodynamic material basis and the pharmacologic mechanism of FXL on treating CAG. As a result, 22 absorbed components were found in rat plasma. Given the results of the absorption analysis of the components, eight pathways associated with CAG development were found. The targets linked to these pathways are the drug targets of FXL in CAG treatment. The components associated with these targets are the potential pharmacodynamic material basis and exert synergy in regulating pathways during CAG treatment.

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“…60 At the same time, it is also related to inflammatory bowel disease, ulcerative colitis, chronic gastritis, and gastric ulcer. 61,62 Some scholars have studied the correlation among diet, microbiome metabolism, and diseases. They found that TMAO-related genes and CRC-related genes share a common genetic pathway in the immune system, cell cycle, cancer pathway, and Wnt signaling pathway.…”

Section: Methylamines (Tma/tmao)mentioning

confidence: 99%

<p>Progress in Research on Colorectal Cancer-Related Microorganisms and Metabolites</p>

Han

Jing

et al. 2020

CMAR

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Intestinal flora is an important component in the human body, which have been reported to be involved in the occurrence and development of colorectal cancer (CRC). Indeed, changes in the intestinal flora in CRC patients compared to those in control subjects have been reported. Several bacterial species have been shown to exhibit the pro-inflammatory and pro-carcinogenic properties, which could consequently have an impact on colorectal carcinogenesis. In this review, we summarize the current knowledge on the potential links between the intestinal microbiota and CRC. We illustrated the mechanisms by which intestinal flora imbalance affects CRC, mainly focusing on inflammation, microbial metabolites, and specific bacteria species. In addition, we discuss how a diet exhibits a strong impact on microbial composition and provides risks for developing CRC. Finally, we describe the potential future directions that are based on intestinal microbiota manipulation for CRC diagnosis and treatment.

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The effect of trimethylamine N-oxide on Helicobacter pylori-induced changes of immunoinflammatory genes expression in gastric epithelial cells

Cited by 28 publications

References 30 publications

Metabolic retroconversion of trimethylamine N-oxide and the gut microbiota

Metabolic retroconversion of trimethylamine N-oxide and the gut microbiota

Exploring the potential pharmacodynamic material basis and pharmacologic mechanism of the Fufang-Xialian-Capsule in chronic atrophic gastritis by network pharmacology approach based on the components absorbed into the blood

<p>Progress in Research on Colorectal Cancer-Related Microorganisms and Metabolites</p>

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