The effect of transport system A and N amino acids and of nerve and epidermal growth factors on the induction of ornithine decarboxylase activity

Rinehart, Clifford A.; Viceps-Madore, Dace; Fong, Wang‐Fun; Ortíz, José G.; Canellakis, E.S.

doi:10.1002/jcp.1041230321

Cited by 41 publications

(29 citation statements)

References 40 publications

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“…Since a-aminoisobutyric acid is a nonmetabolizable amino acid, the induction of ornithine decarboxylase activity is not due to the participa-* tion of a-aminoisobutyric acid in protein synthesis or to other reactions involving covalent modification of a-amino-0 isobutyric acid. (21). The of 0.1 AM insulin.…”

Section: Resultsmentioning

confidence: 99%

“…Intracellular association becomes more interesting in the light of the fact s trichloroacetic acid-soluble radioobtained with the nonmetabolizable that the ornithine decarboxyelase-inducing amino acids are log, a-aminoisobutyric acid. o, transported via Na+-dependent transport systems (21). e with insulin.…”

Section: Resultsmentioning

confidence: 99%

“…In another portion, the ornithine decarboxylase activity was determined at saturating substrate concentrations (0.55 mM) as described (13); one unit represents 1 nmol of CO2 released per hour. Other materials and methods used in this study have been published (21).…”

Section: Methodsmentioning

confidence: 99%

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Induction of ornithine decarboxylase activity by insulin and growth factors is mediated by amino acids.

Rinehart¹,

Canellakis²

1985

Proc. Natl. Acad. Sci. U.S.A.

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The polypeptide growth factors, nerve growth factor, epidermal growth factor, and platelet-derived growth factor, as well as insulin do not induce ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) unless a minimal concentration of an ornithine decarboxylase-inducing amino acid, such as asparagine, is present in the medium. The effects of the growth factors were studied in appropriately responsive cell lines: pheochromocytoma (PC12) cells for nerve and epidermal growth factors, fibroblasts (NIH 3T3) for platelet-derived growth factor, and fibroblasts and hepatoma (KRC-7) cells for insulin. The nonmetabolizable amino acid analog alpha-aminoisobutyric acid can replace asparagine, indicating that the covalent modification of the inducing amino acid is not necessary for the induction of ornithine decarboxylase by these growth factors. For the same intracellular concentration of the inducing amino acid, the presence of the growth factors induces higher levels of ornithine decarboxylase. The evidence indicates that these growth factors do not induce ornithine decarboxylase by raising the intracellular concentration of amino acids but rather act synergistically with the inducing amino acid. Evidence is provided that the induction of polyamine-dependent growth by these growth factors is mediated by amino acids. The relationship of these results to the A and N amino acid transport systems and to the Na+ influxes in relation to growth is discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Induction of ornithine decarboxylase activity by insulin and growth factors is mediated by amino acids.

Rinehart¹,

Canellakis²

1985

Proc. Natl. Acad. Sci. U.S.A.

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“…In subsequent studies it was shown that nonmetabolizable analogs of the group 'A' amino acids also elevate ODC (Viceps-Madore et al, 1982). These investigators further reported that amino acids that are transported through a Na+-dependent cotransport system must be present in EBSS for CAMP, epidermal growth factor, and nerve growth factor to enhance ODC expression (Rinehart et al, 1985). These results suggested that ODC induction might be linked to the transport system of some amino acids.…”

Section: Discussionmentioning

confidence: 92%

Glucose elevates ornithine decarboxylase expression in vero cells

Lundgren

Prokay

1988

Journal Cellular Physiology

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The addition of Earle's balanced salt solution (EBSS) of amino acids that are transported by a Na+-dependent cotransport system was not required by Vero cells for ornithine decarboxylase (ODC:EC 4.1.1.17) amplification. Vero cell ODC activity was elevated tenfold above basal levels when confluent cells were incubated for 5 hr in EBSS alone. ODC activity increased as a function of the incubation time in EBSS and was not elevated above basal enzyme levels when cells were incubated in EBSS minus glucose. ODC expression increased as a function of the glucose concentration in EBSS, with 20 mM glucose producing a 90-fold increase in ODC activity. ODC expression is more responsive to glucose in high-density quiescent cultures than in low-density growing cultures. Enhanced ODC expression by glucose depended on Na+ and K+ concentrations. The specific activity of ODC was also elevated above basal levels when mannose or fructose replaced glucose in EBSS. The addition of alanine or asparagine to EBSS enhanced ODC activity above levels obtained with EBSS containing standard (5.5 mM) glucose concentrations. In the absence of glucose, alanine was more effective than asparagine in enhancing ODC expression. These results suggest that the transport of amino acids is not an absolute requirement for Vero cell ODC expression and that ODC expression is linked to changes in cellular energetics and/or ion fluxes.

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“…Ornithine decarboxylase activity in various cell types is induced by specific amino acids (Rinehart et al, 1985). In mouse neuroblastoma cells, the most potent inducers are asparagine, glutamine, and serine.…”

Section: Atp Content Does Not Change In Cells Protected By Amino Acidmentioning

confidence: 99%

Modulation of cellular heat sensitivity by specific amino acids

Vidair

Dewey

1987

Journal Cellular Physiology

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When either plateau-phase or exponentially growing Chinese hamster ovary (CHO) cells are incubated in amino acid-free medium, the cells become sensitized to killing by heat. For cells deprived of amino acids for 12 h survival decreases from 1 X 10(-2) for controls to 1 X 10(-6) for the deprived cells, following heating at 45 degrees C for 38 min. The survival of these sensitized cells is rapidly increased by the addition of a single amino acid just prior to heating. Of the 21 amino acids which are added in purified form to make McCoy's 5a medium, 12 show no protective effect, four have a small protective effect, and either alanine, asparagine, glutamine, serine, or theronine raise survival to a level similar to that of the control cells. The nonmetabolizable alanine analogue, 2-aminoisobutyric acid (AIB), increases survival of amino acid-deprived cells as effectively as each member of the group of five listed above, suggesting that metabolic conversion of the amino acids is not required for their protective effect. The data suggest that an increase in the intracellular concentrations of specific amino acids, independent of any change in cellular ATP content or the rate of protein synthesis, enables these cells to become quickly more resistant to killing by heat. We also conclude that the amino acid concentrations in poorly vascularized regions of some tumors should be considered, along with the oxygen, glucose, and proton concentrations, as factors which determine cellular survival following hyperthermia.

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The effect of transport system A and N amino acids and of nerve and epidermal growth factors on the induction of ornithine decarboxylase activity

Cited by 41 publications

References 40 publications

Induction of ornithine decarboxylase activity by insulin and growth factors is mediated by amino acids.

Induction of ornithine decarboxylase activity by insulin and growth factors is mediated by amino acids.

Glucose elevates ornithine decarboxylase expression in vero cells

Modulation of cellular heat sensitivity by specific amino acids

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