The effect of transient proanthocyanidins preconditioning on the cross-linking and mechanical properties of demineralized dentin

Abstract

Proanthocyanidin-based preconditioners were prepared by adding powdered proanthocyanidins-rich grape seed extract to various solvents at different concentrations. Demineralized dentin specimens were preconditioned for 20, 30, 60 or 120 s, followed by the evaluation of their cross-linking degree, mechanical properties and micromorphology. The cross-linking degree of the demineralized dentin collagen exhibited concentration- and time- dependent increase after preconditioning treatment, irrespective of the preconditioner and the solvent. When treated for the same exposure time, specimens after 15% proanthocyanidins preconditioning resulted in the highest mean ultimate tensile strength compared with all the other groups tested. Five percent glutaraldehyde control group produced the highest cross-linking degree, but the ultimate tensile strength was lower than that of 15% proanthocyanidins group. The field emission scanning electron microscopy confirmed that the demineralized dentin collagen was in a homogeneous and regular arrangement after preconditioning and maintained expanding, regardless of the surface moisture conditions.

HPLC detection of loss rate and cell migration of HUVECs in a proanthocyanidin cross-linked recombinant human collagen-peptide (RHC)–chitosan scaffold

“…Ninhydrin assay: The specimens were also subjected to a modified ninhydrin assay based on the previous report [26,27]. Briefly, ninhydrin was dissolved in PBS (0.3M, pH 7.4) with 0.3% fructose, specimens were heated with ninfydrin solution at 85℃ for 20min, 45% alcohol was added to the reaction system and the optical absorbance was recorded with a Multifunctional Microplate Reader (TECAN infinite M200 PRO, Austria) at 570nm.…”

Biomodification to dentin by a natural crosslinker improved the resin–dentin bonds

“…It was found that collagen cross-linking treatment by PA-based preconditioners in a clinically applicable duration increased the cross-linking degree and ultimate tensile strength of demineralised dentin. 21 Therefore the aim of this study was to evaluate whether transient PA preconditioning can also improve dentin bonding characteristics, and whether the type of solvent, concentration of PA and the exposure duration will have influence on resin-dentin bonds. The null hypothesis tested was that the transient use of PA would not significantly affect resin-dentin bonds when compared to a non-treated group, regardless of the type of solvent, concentration and the exposure duration.…”

Effect of grape seed extract on the bond strength and durability of resin-dentin interface

“…When PA interacts with collagen, it is capable of inducing inter-and intra-fibrillar, as well as inter-microfibrillar cross-links in the collagen matrix [43,53,54]. Numerous free phenyl hydroxyl groups are present in PA [55] which can create bridge-type hydrogen bonds with the side chains of hydroxyl, carboxyl, amino or amide groups of the collagen molecules [56,57].…”