The effect of transgenic cucumbers expressing
thaumatin on selected immunity parameters in rats

Kosieradzka, I.; Sawosz, Ewa; Winnicka, A.; Kluciński, W.; Malepszy, S.; Szwacka, M.; Pastuszewska, B.

doi:10.22358/jafs/70313/2004

Cited by 4 publications

(4 citation statements)

References 6 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When interpreting these results, however, it should be taken into consideration that the sensitivity of the diagnostic method used may be too low for detection of a small concentration of transgenic DNA in the examined material. Such a result of the experiment supports the possibility of safe utilization of cucumbers with taumatin in the diet, found in previous experiments showing no influence of GMO on growth and selected health parameters of model animals (Kosieradzka et al, 2001(Kosieradzka et al, , 2004. KOSIERADZKA I.…”

Section: Discussionsupporting

confidence: 86%

Evaluation of the possibility of horizontal gene transfer and accumulation of transgenic DNA from the diet in the bodies of rats

Kosieradzka¹,

Vasko²,

Szwacka³

et al. 2010

J. Anim. Feed Sci.

Self Cite

View full text Add to dashboard Cite

The procedures for GMO safety tests include traceability of transgenic protein and transgenic DNA if the plant constitutes a component in the diet for an animal. This is due to the possibility of horizontal transfer of genes, accumulation of transgenic DNA in consumer's organs, or induction of antibiotic resistance in gastrointestinal tract microflora. The last possibility is related to the use of marker genes in the process of transformation. In an in vivo experiment conducted on laboratory rats with the use of transgenic cucumbers expressing the pre-prothaumatin gene, the presence of transgenic DNA in the tissue of kidneys and liver was not detected. Resistance to neomycin of gastrointestinal tract microflora of the rats fed the GMO diet was not found, despite the use of marker genes (npt II) in the process of transformation of the investigated plants.

show abstract

Section: Discussionsupporting

confidence: 86%

Evaluation of the possibility of horizontal gene transfer and accumulation of transgenic DNA from the diet in the bodies of rats

Kosieradzka¹,

Vasko²,

Szwacka³

et al. 2010

J. Anim. Feed Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Administration of GM tomatoes, containing thaumatin, did not increase the level of immunoglobulins synthesized in the organism as a response to allergen presence in the diet (protein, inducing sensitiveness) (Table 4). In the experiments of Kosieradzka et al [2004], 15% addition of GM cucumbers with thaumatin expression in diets for the rats had the immunomodulating effect; it affected differences in values of non-specific immunity parameters.…”

Section: Discussionmentioning

confidence: 95%

Nutritive and Dietetic Value of Genetically Modified Tomatoes Expressing Thaumatin Gene

Kosieradzka¹,

Vasko²,

Bartoszewski³

et al. 2014

Pol. J. Food Nutr. Sci.

View full text Add to dashboard Cite

Genetically-modified (GM) tomatoes, carrying thaumatin gene encoding sweet-tasting protein may be a component of diet with high sensory values, constituting a valuable source of nutrients and substances with a health-promoting role. Good utilization and a lack of the effect on animal growth, value of hematological parameters, concentration of immunoglobulins and most of chemical blood parameters of laboratory rats were demonstrated in the nutritional studies on fruits of tomato GM plants. The biological response of the rats receiving GMO or its isogenic equivalent in the diet was recognized as similar. However, the unfavourable effect of the diets containing addition of tomatoes with the recombined thaumatin on the degree of oxidative degradation of DNA of rats liver was recorded. At the same time, the discussed dietary component had no effect on values of the remaining parameters of the oxidative status of tissue of the above mentioned organ and its histological image.

show abstract

“…The safety of food containing transgenic cucumber or transgenic tomato expressing thaumatin II gene so far has included an examination of compositional equivalence as well as nutritional studies with animals (Kosieradzka and others 2001, 2004, 2010; Twardowska 2003; Vasko 2010).…”

Section: Food Safety Assessment Of Transgenic Cucumber and Tomatomentioning

confidence: 99%

“…As biosynthesis of a new protein may reveal immunogenic activity, the response of the immune system of laboratory rats receiving transgenic fruit‐containing diets was therefore assessed on the basis of nonspecific resistance parameters. It was found that the addition of transgenic fruits to the diet caused an increase in the values of selected parameters of nonspecific immunity, namely in the number of neutrophils that activated the oxidation process after phorbol‐12‐myristate‐13‐acetate (PMA) and N ‐formyl‐L‐methionyl‐L‐leucyl‐phenylalanine (fMLP) stimulation, as well as in the activity of neutrophils; there was higher neutrophil fluorescence intensity when E. coli was used as the chemotactic factor (Kosieradzka and others 2004). An increase in the nonspecific immunity of rats may be a consequence of a change in the chemical composition of transgenic fruits.…”

Section: Food Safety Assessment Of Transgenic Cucumber and Tomatomentioning

confidence: 99%

Genetically Modified Crops Expressing 35S‐Thaumatin II Transgene: Sensory Properties and Food Safety Aspects

Szwacka¹,

Burza²,

Zawirska‐Wojtasiak³

et al. 2012

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Obesity and diabetes have led consumers to increasingly seek out products containing low-calorie sweeteners. In this context, the intense sweeteners, especially plant proteins that interact with the taste receptors, are playing a growing role. Among the sweet-tasting proteins thaumatin seems the most promising. Production of thaumatin by transgenic plants might be an interesting alternative to the extraction of this product from the natural source. Another possible application of the unusual properties of thaumatin is to produce transgenic plants containing a protein capable of inducing a sweet taste phenotype. The taste of 5 crop species, cucumber, pear, potato, strawberry, and tomato, was improved by expressing the thaumatin II gene under control of the 35S promoter. Only in the case of transgenic cucumber were sensory assessments carried out for several cultivation cycles. Also, processed fruits of transgenic cucumber, as well as fresh-cut transgenic cucumber salads, were the subject of sensory analysis. Transgenic cucumber expression of the thaumatin II gene resulted not only in sweeter taste, in comparison with the control, but also in higher aroma acceptability. Therefore, the authors of this article have summarized sensory properties of transgenic crops expressing the thaumatin II gene, placing special emphasis on the sensory characteristics of transgenic cucumber. Consumers often perceive disparagingly food deriving from genetically modified (GM) plants, partly because of some concerns about unintended effects. To address such concerns, this article also summarizes the food safety evaluation of transgenic crops expressing the thaumatin II gene. Future research directions are suggested.

show abstract

The effect of transgenic cucumbers expressing thaumatin on selected immunity parameters in rats

Cited by 4 publications

References 6 publications

Evaluation of the possibility of horizontal gene transfer and accumulation of transgenic DNA from the diet in the bodies of rats

Evaluation of the possibility of horizontal gene transfer and accumulation of transgenic DNA from the diet in the bodies of rats

Nutritive and Dietetic Value of Genetically Modified Tomatoes Expressing Thaumatin Gene

Genetically Modified Crops Expressing 35S‐Thaumatin II Transgene: Sensory Properties and Food Safety Aspects

Contact Info

Product

Resources

About