The effect of trans-10, cis-12 conjugated linoleic acid on gene expression profiles related to lipid metabolism in human intestinal-like Caco-2 cells

Abstract

We conducted an in-depth investigation of the effects of conjugated linoleic acid (CLA) on the expression of key metabolic genes and genes of known importance in intestinal lipid metabolism using the Caco-2 cell model. Cells were treated with 80 mumol/L of linoleic acid (control), trans-10, cis-12 CLA or cis-9, trans-11 CLA. RNA was isolated from the cells, labelled and hybridized to the Affymetrix U133 2.0 Plus arrays (n = 3). Data and functional analysis were preformed using Bioconductor. Gene ontology analysis (GO) revealed a significant enrichment (P < 0.0001) for the GO term lipid metabolism with genes up-regulated by trans-10, cis-12 CLA. Trans-10, cis-12 CLA, but not cis-9, trans-11 CLA, altered the expression of a number of genes involved in lipid transport, fatty acid metabolism, lipolysis, beta-oxidation, steroid metabolism, cholesterol biosynthesis, membrane lipid metabolism, gluconeogenesis and the citrate cycle. These observations warrant further investigation to understand their potential role in the metabolic syndrome.

The trans fatty acid elaidate affects the global DNA methylation profile of cultured cells and in vivo

“…The transcriptional profile induced by EA in THP-1 cells is markedly adipogenic compared to OA, in accordance with published data obtained with EA or trans conjugated FAs [ 19 , 20 ]. tFA-induced adipogenesis is accompanied by hepatic steatosis [ 19 , 21 – 24 ], which may share underlying mechanisms with the fatty lesions observed here in one mammary gland of EA-treated dams.…”

Effects of (-)-epigallocatechin-3-O-gallate on expression of gluconeogenesisrelated genes in the mouse duodenum

“…5B). The previous finding that the gene expression of both PEPCK and HNF4α was increased in Caco-2 cells by conjugated linoleic acid (18) supports the idea that modulation of the HNF4α gene affects the PEPCK gene expression in these cells. Among various tea catechins (Fig.…”

“…These cells have been used for the intestinal expression of gluconeogenic enzymes, G6Pase and PEPCK, and that of related transcription factors, HNF1α and HNF4α (5,9,18,24,35). When stimulated with dcAMP/ Dex, the gene expression of G6Pase and PEPCK was up-regulated (Fig.…”

Acute and chronic effects of dietary fatty acids on cholecystokinin expression, storage and secretion in enteroendocrine STC‐1 cells

“…The concentrations of FAs used were much lower than those present in food and similar to the concentrations present in the lumen of the human small intestine [32,33]. We extend earlier work by not only comparing effects on acute CCK secretion, but also cellular CCK content, CCK mRNA, cellular DNA synthesis, cell viability and cell toxicity.…”