The effect of topical tretinoin on tissue strength and skin components in a murine incisional wound model

Muehlberger, Thomas; Moresi, J. Margaret; Schwarze, Hartmut; Hristopoulos, Georgios; Laenger, Florian; Wong, Lesley

doi:10.1016/j.jaad.2004.08.059

Cited by 25 publications

(17 citation statements)

References 30 publications

(23 reference statements)

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“…The gap junction protein connexin-43 is upregulated by UVR (35) and overexpression of Wound contraction and incisional wound breaking strength were not influenced by UVR. The breaking strength values of the control incisions agree with those reported by other investigators (38). In contrast, chronic UVR causes dermal changes in experimental and human models.…”

Section: Discussionsupporting

confidence: 90%

Acute Ultraviolet Radiation Perturbs Epithelialization but not the Biomechanical Strength of Full‐thickness Cutaneous Wounds

Danielsen

Lerche

Wulf

et al. 2016

Photochem & Photobiology

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We hypothesized that priming of the skin with ultraviolet radiation (UVR) before being injured would enhance wound healing. Four groups, each comprising 20 immunocompetent hairless mice, were exposed to simulated solar irradiation in escalating UVR doses; 0 standard erythema dose (SED) = control, 1 SED, 3 SED and 5 SED. Twenty-four hours after UV irradiation, inflammation was quantified by skin reflectance (erythema) and myeloperoxidase (MPO) tissue levels, and two 6 mm full-thickness excisional wounds and one 3 cm incisional wound were inflicted. Epidermal hyperplasia was assessed by quantitative histology. Five days after wounding, wound coverage by neoepithelium and wound width of the excisional wounds was quantified in hematoxylin-eosin sections, and breaking strength was measured in strips from incisional wounds. Erythema (P < 0.001), MPO levels (P < 0.0005) and epidermal cell layers (P < 0.001) increased dose-dependently by UV exposure of dorsal skin. In the excisional wounds, epithelial coverage decreased (P = 0.024) by increasing the UVR dose, whereas there was no significant difference (P = 0.765) in wound MPO levels. Neither wound width (P = 0.850) nor breaking strength (P = 0.320) differed among the groups. Solar-simulated UVR 24 h before wounding impaired epithelialization but was not detrimental for surgical incisional wound healing.

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Section: Discussionsupporting

confidence: 90%

Acute Ultraviolet Radiation Perturbs Epithelialization but not the Biomechanical Strength of Full‐thickness Cutaneous Wounds

Danielsen

Lerche

Wulf

et al. 2016

Photochem & Photobiology

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“…It was previously demonstrated that increased dermal inflammatory response in the early phase of wound healing may result in decreased collagen content and compromised wound tensile strength [23]. In our study, we have not noticed any significant changes in the level of leukocyte infiltration in different treatments.…”

Section: Discussioncontrasting

confidence: 48%

Effect of Halofuginone, a Collagen α1(I) Inhibitor, on Wound Healing in Normal and Irradiated Skin: Implication for Hematopoietic Stem Cell Transplantation

et al. 2007

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It is a major interest in the field of hematopoietic stem cell transplantation to reduce scarring of healing wounds with overdeposition of collagen due to radiation injury or graft-versus-host disease. Halofuginone (HF) inhibits collagen α1(I) gene expression and overdeposition of collagen. We examined the effect of HF on the healing of full-depth incision wounds inflicted in normal skin or skin areas compromised by local preirradiation with 18 Gy. Preirradiation significantly decreased the tensile strength of the healing wounds at day 14 (by ∼60%, p < 0.0001). In contrast, HF treatment did not significantly decrease the strength of wounds inflicted in both normal and preirradiated skin. Histological evaluation revealed that HF induced moderate thinning of the dermis accompanied by elevated thickness of the epidermis and enhanced rejoining of subdermal muscles in the wound area. HF only minimally reduced total collagen deposition in both groups, with minor changes in the level of more matured fibrillar collagen network. Our study demonstrates that HF does not significantly affect wound strength. This encourages the possible use of HF as an antifibrotic agent with minimal complications for post-hematopoietic stem cell transplantation complications including radiation toxicity and graft-versus-host disease.

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“…For each specimen, three separated sections were randomly selected for histological evaluation. The fibroblast proliferation, neovascularization, granulation tissue, epithelialization and collagen deposition were evaluated semi-quantitatively using accepted wound scoring protocols published previously (Muehlberger et al 2005;Qiu et al 2007). Wound healing scores were assigned a value between 0 and 4 as follows: 0 = none; 1 = rare or minimal; 2 = moderate; 3 = abundant; and 4 = severe or marked.…”

Section: Histological Evaluationmentioning

confidence: 99%

Extracorporeal Shock Wave Treatment Improves Incisional Wound Healing in Diabetic Rats

Yang

Luo

Yan

et al. 2011

Tohoku J. Exp. Med.

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Impaired wound healing in surgical patients with diabetes increases the incidence of infection, prolongs hospitalization, and even increases the rate of mortality. Low-energy extracorporeal shock wave treatment (ESWT) was reported to accelerate chronic wound healing by promoting revascularization and tissue regeneration; however, it is not known if ESWT could also improve healing of acute surgical incisional wounds in diabetes. In this study, using a rat model of diabetes, we investigated the effect of low-energy ESWT on collagen content in wound tissues and its efficacy in incisional wound healing. A single dorsal incisional wound was inflicted in streptozotocin-induced diabetic rats, and they received ESWT at different time post-wounding. Rats were sacrificed on days 7 and 14 post-wounding. Wound breaking strength, hydroxyproline content, histological characteristics and the expression of transforming growth factor beta 1 (TGF-β1) were analyzed. As a result, the wound breaking strength was significantly enhanced and the hydroxyproline content in wound tissues was increased at each time point examined. The number of fibroblasts was significantly increased, and the new collagen fibers were more abundant at the wound site after ESWT. Furthermore, the expression of TGF-β1 was up-regulated after ESWT on day 7 post-wounding. These results suggest that low-energy ESWT can increase collagen content, enhance wound breaking strength and improve the healing of incisional wound in diabetic rats. The increased collagen content may be attributed, at least in part, to the up-regulation of TGF-β1 expression in wound tissues.

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The effect of topical tretinoin on tissue strength and skin components in a murine incisional wound model

Cited by 25 publications

References 30 publications

Acute Ultraviolet Radiation Perturbs Epithelialization but not the Biomechanical Strength of Full‐thickness Cutaneous Wounds

Acute Ultraviolet Radiation Perturbs Epithelialization but not the Biomechanical Strength of Full‐thickness Cutaneous Wounds

Effect of Halofuginone, a Collagen α1(I) Inhibitor, on Wound Healing in Normal and Irradiated Skin: Implication for Hematopoietic Stem Cell Transplantation

Extracorporeal Shock Wave Treatment Improves Incisional Wound Healing in Diabetic Rats

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