The effect of tocopheryl phosphates on atherosclerosis progression in rabbits fed with a high cholesterol diet

Negis, Yesim; Aytan, Nurgül; Özer, Nesrin Kartal; Ogru, Esra; Libinaki, Roksan; Gianello, Robert; Azzi, Angelo; Zingg, Jean‐Marc

doi:10.1016/j.abb.2006.02.027

Cited by 56 publications

(81 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, it seems that the antioxidant activities of TocP are not mainly involved in the repressive effects on tumor invasion, and the anti-invasive effect of TocP seems to be due to the effect of the parent compound Toc. It has been also shown that TocP is highly resistant to hydrolysis by both chemical and enzymatic actions, which indicates that its effect is not related to the hydrolytic conversion to free tocopherol (4,7,9,10,43). Furthermore, it was shown that TocP has a more marked potency than the non-esterified molecule in several studies (4,(8)(9)(10).…”

Section: Discussionmentioning

confidence: 98%

“…Furthermore, several studies have also indicated that TocP has been shown to be more effective than Toc in the regulation of cell properties (4,(8)(9)(10). Thus, the possibility that the active form of Toc may be TocP has been considered (4,9). Although various effects of TocP have been reported as mentioned above, little is known about its anti-cancer effects.…”

Section: Introductionmentioning

confidence: 99%

“…For example, TocP can reduce myocardial ischemia/reperfusion injury (5), protect against ultraviolet B-induced damage in cultured mouse skin (6), suppress lipid peroxidation and induction of erythrocyte hemolysis (7), induce cell death, inhibit cell proliferation and provoke apoptosis in osteosarcoma cells (3), down-regulate the scavenger receptor CD36 (4,8,9), and exert atheroscleroticpreventing effects (8,9). Furthermore, several studies have also indicated that TocP has been shown to be more effective than Toc in the regulation of cell properties (4,(8)(9)(10). Thus, the possibility that the active form of Toc may be TocP has been considered (4,9).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

α-tocopheryl phosphate suppresses tumor invasion concurrently with dynamic morphological changes and delocalization of cortactin from invadopodia

Saitoh

Yumoto²,

Miwa³

2009

Int J Oncol

View full text Add to dashboard Cite

Abstract. ·-tocopheryl phosphate (TocP) has been elucidated for diverse effectiveness through unequivocal mechanisms independent of its conversion to ·-tocopherol (Toc). The present study showed that TocP, in a dose-dependent manner, repressed invasion of human fibrosarcoma HT-1080 cells through the basement membrane, more markedly than Toc and several other Toc derivatives, although no cytotoxic effect was shown in either HT-1080 cells or human skin dermal fibroblastic cells DUMS-16. TocP diminished intracellular reactive oxygen species such as peroxides, lipid hydroperoxides and superoxide anion radicals in HT-1080 cells, but the effects were nearly equal or less than those of Toc. TocP suppressed either the cell motility or adhesion to extracellular matrix (ECM), and induced diagnostic morphological changes such as appearance of numerous spikeshaped processes and vesicles. Localization of cortactin in the membrane of invadopodia, which dispatches signal transduction for tumor invasion, was lost by TocP as shown by immunostaining. Similar result was obtained also by Western blots showing that cortactin in the membrane fraction was markedly diminished by TocP in contrast to unchanged cortactin amounts in other subcellular fractions including nuclei, cytoplasms and cytoskeletons. In contrast, TocP scarcely affected the invasiveness-related gelatinases MMP-2/9 and cell membrane fluidity. Thus, TocP markedly exerted anti-invasive activities through decrease in cell motility, repression of adhesion to the ECM, cell morphological changes and, especially, alteration of cortactin distribution. Moreover, TocP also preferentially inhibited tumor invasion without cytotoxicity to normal cells derived from the corresponding tissue type. Consequently, TocP is suggested as a potent tumor-invasion suppressor.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

α-tocopheryl phosphate suppresses tumor invasion concurrently with dynamic morphological changes and delocalization of cortactin from invadopodia

Saitoh

Yumoto²,

Miwa³

2009

Int J Oncol

View full text Add to dashboard Cite

show abstract

“…The vitamin E (a-tocopherol) derivative, a-tocopheryl phosphate is present in measurable amounts in plasma, tissues, and cells. Animals and humans supplementation has shown that tocopheryl phosphate can reach plasma concentrations similar to tocopherol in rabbits [40] and in humans (Zingg et al, unpublished). Furthermore, its dephosphorylation is not efficient.…”

Section: Phosphorylation: a Way To Activate Vitamin E Signaling And Fmentioning

confidence: 99%

“…Tocopheryl phosphate modulates the activity of several enzymes; in cell culture, it affects proliferation, signal transduction, and gene expression at concentrations lower than tocopherol (Zingg and coworkers, [41]). In high cholesterol fed rabbits and LDL knockout mice tocopheryl phosphate prevents atherosclerosis at much lower concentrations than tocopherol [40]. In the ischemia/reperfusion injury of rat heart [42], tocopheryl phosphate may act at the molecular level as an active lipid mediator similar to phosphatidylinositol therefore influencing signal transduction and gene expression.…”

Section: Phosphorylation: a Way To Activate Vitamin E Signaling And Fmentioning

confidence: 99%

Present trends in vitamin E research

2010

Self Cite

View full text Add to dashboard Cite

Abstract.Nearly after one century of research and thousands of publications, the physiological function(s) of vitamin E remain unclear. Available evidence suggests a role in cell homeostasis that occurs through the modulation of specific signaling pathways and genes involved in proliferative, metabolic, inflammatory, and antioxidant pathways. Vitamin E presence in the human body is under close metabolic control so that only a-tocopherol and, to a lower extent, c-tocopherol are retained and delivered to tissues. Other vitamin E forms that are not retained in the body in significant amounts, exhibit responses in vitro that are different form those of a-tocopherol and may include tumor cell specific toxicity and apoptosis. These responses provide a therapeutic potential for these minor forms, either as such or metabolically modified, to produce bioactive metabolites. These cellular effects go beyond the properties of lipophilic antioxidant attributed to a-tocopherol particularly investigated for its alleged protective role in atherosclerosis or other oxidative stress conditions. Understanding signaling and gene expression effects of vitamin E could help assign a physiological role to this vitamin, which will be discussed in this review. Besides vitamin E signaling, attention will be given to tocotrienols as one of the emerging topics in vitamin E research and a critical re-examination of the most recent clinical trials will be provided together with the potential use of vitamin E in disease prevention and therapy.

show abstract

Dietary antioxidants and bioflavonoids in atherosclerosis and angiogenesis

Meydani¹,

Azzi²

2017

Nutrigenomics and Proteomics in Health and Disease

View full text Add to dashboard Cite

The effect of tocopheryl phosphates on atherosclerosis progression in rabbits fed with a high cholesterol diet

Cited by 56 publications

References 11 publications

α-tocopheryl phosphate suppresses tumor invasion concurrently with dynamic morphological changes and delocalization of cortactin from invadopodia

α-tocopheryl phosphate suppresses tumor invasion concurrently with dynamic morphological changes and delocalization of cortactin from invadopodia

Present trends in vitamin E research

Dietary antioxidants and bioflavonoids in atherosclerosis and angiogenesis

Contact Info

Product

Resources

About