The effect of timing of embryonic progression on chromosomal abnormality

Kroener, Lindsay; Ambartsumyan, G.; Briton-Jones, Christine; Dumesic, Daniel A.; Surrey, M.; Munné, Santiago; Hill, David L.

doi:10.1016/j.fertnstert.2012.06.014

Cited by 66 publications

(46 citation statements)

References 23 publications

(29 reference statements)

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“…Similar to a prior study [8] comparing the rate of embryo development with aneuploidy rates, this study did find that as a whole, slower developing embryos on day 5 had a higher aneuploidy rate compared with day 5 expanded blastocysts even after controlling for maternal age. Maternal age was controlled for in this study by both stratifying embryo cohorts by maternal age, and using a multivariable logistic regression model, with both analyses reaching similar conclusions.…”

Section: Discussionsupporting

confidence: 84%

“…Kroener et al demonstrated that after a day 3 biopsy, day 5 morulae have a significantly higher overall aneuploidy rate than day 5 blastocysts [8]. However, the subset of day 5 morulae that became blastocysts on day 6-the most clinically important embryos-did not have any difference in aneuploidy rates compared to day 5 blastocysts, suggesting that the biopsy of day 5 morulae may be just as worthwhile as the biopsy of day 5 blastocysts when aiming for a fresh day 6 transfer.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Aneuploidy rates and blastocyst formation after biopsy of morulae and early blastocysts on day 5

Kort

Lathi

Brookfield

et al. 2015

J Assist Reprod Genet

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Purpose Studies have demonstrated high implantation rates after trophectoderm biopsy of day 5 expanded blastocysts. However, biopsy of cleavage stage embryos may adversely affect embryo development and implantation. No studies have assessed the utility of day 5 morulae and early blastocyst biopsy. This study sought to better understand these slower embryos' aneuploidy rates and implantation potential. Methods This was a retrospective review of all autologous IVF cycles utilizing PGS at a single academic infertility center. Results The biopsy of day 5 morulae and early blastocysts provided 22 % additional euploid blastocysts available for fresh day 6 transfer compared to day 5 biopsy of only expanded blastocysts. Aneuploidy did correlate with embryo stage on day 5, even after controlling for maternal age, with 16 % of morulae and 35 % of blastocysts being euploid. The majority (83 %) of euploid morulae progressed to the blastocyst stage by day 6. Experience transferring slower developing embryos is limited, but preliminary pregnancy and implantation rates appear similar to euploid embryos biopsied as expanded blastocysts.Conclusions The biopsy of all non-arrested embryos on day 5 provides genetic information for all blastocysts on day 6, increasing the pool of euploid blastocysts available for fresh transfer and avoiding the need to cryopreserve developmentally competent embryos without genetic information.

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Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Aneuploidy rates and blastocyst formation after biopsy of morulae and early blastocysts on day 5

Kort

Lathi

Brookfield

et al. 2015

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…It was suggested that the timing of blastocyst formation is not linked to or affected by chromosomal abnormalities and embryos reaching the expanded blastocyst stage on day 6 or 7 have a similar risk of being aneuploid as faster growing ones [66,67]. At the same time, it was evidenced that lower-quality euploid embryos yield the same ongoing implantation rate compared with blastocysts of good morphological quality [68].…”

Section: Discussionmentioning

confidence: 99%

The endometrial preparation for frozen-thawed euploid blastocyst transfer: a prospective randomized trial comparing clinical results from natural modified cycle and exogenous hormone stimulation with GnRH agonist

Greco

Litwicka

Arrivi

et al. 2016

J Assist Reprod Genet

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Purpose The aim of the study was to evaluate two methods of endometrial preparation for frozen-thawed single euploid blastocyst transfer: modified natural and artificial cycle with GnRH-agonist pituitary suppression. Methods In this prospective, controlled randomized trial, a total of 236 patients undergoing infertility treatment were randomized in 1:1 ratio; 118 received a frozen-thawed single euploid blastocyst transfer in a modified natural cycle and 118 in an artificial cycle with GnRH-agonist pituitary suppression. In the artificial protocol, GnRH-agonist combined with estradiol valerate was administered. In the natural protocol, only final oocyte maturation was induced using human chorionic gonadotropin administration. The primary end-points were the clinical pregnancy and implantation rates; the secondary end-points were the cost-benefit in terms of drug cost and the number of visits and the woman psychological distress caused by the treatment. Results No significant differences were found in clinical pregnancy, implantation, and miscarriage rates between protocols. The number of clinical and ultrasound controls and the number of laboratory dosages and venous samplings were similar in both study groups. No significant differences were found between the groups in the anxiety and depression values before the start of treatment, on the days of progesterone administration, the blastocyst transfer, and pregnancy test. Conclusions The findings of this study evidence that in case of frozen-thawed single euploid blastocyst transfer, both protocols are equally effective in terms of clinical outcomes, costbenefit, and patient compliance. The choice of endometrial preparation protocol should be based on women menstrual and ovulatory characteristics or otherwise on patient need for cycle planning. Trial registration www.clinicaltrials.gov with number NCT02378584

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“…One important difference between previous randomized trials and IVF performed at present is the increasing utilization of extended embryo culture and blastocyst transfer. Higher embryo implantation rates have been demonstrated for embryos cultured for 5-6 days to the blastocyst stage (15), which is likely because aneuploid embryos are more likely to arrest in development during extended culture, thus enriching the blastocyst-stage embryos with euploid embryos capable of producing a viable pregnancy (16,17). We found that clinics performing a high rate of eSET had higher utilization of blastocyst ETs and higher embryo implantation rates, which likely accounts for their ability to maintain equal clinic-level live birth rates despite the slightly lower cycle-level live birth rates associated with eSET.…”

Section: Discussionmentioning

confidence: 99%

Elective single embryo transfer in women less than age 38 years reduces multiple birth rates, but not live birth rates, in United States fertility clinics

Mancuso

Boulet

Duran

et al. 2016

Fertility and Sterility

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The effect of timing of embryonic progression on chromosomal abnormality

Cited by 66 publications

References 23 publications

Aneuploidy rates and blastocyst formation after biopsy of morulae and early blastocysts on day 5

Aneuploidy rates and blastocyst formation after biopsy of morulae and early blastocysts on day 5

The endometrial preparation for frozen-thawed euploid blastocyst transfer: a prospective randomized trial comparing clinical results from natural modified cycle and exogenous hormone stimulation with GnRH agonist

Elective single embryo transfer in women less than age 38 years reduces multiple birth rates, but not live birth rates, in United States fertility clinics

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