The effect of thrombin inhibition in a rat arterial thrombosis model

Broersma, Robert J.; Kutcher, Louis W.; Heminger, Eileen F.

doi:10.1016/0049-3848(91)90341-s

Cited by 37 publications

(14 citation statements)

References 9 publications

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“…At the initial stages of the search for new antithrombotics smallanimal models are preferable. Rat models of arterial injury induced by electric current [6], ferric chloride [7] or ferrous chloride [8] have found wide application. Ferric or ferrous chloride models proved to be adequate for the investigation of the antioxidant effect since they imply the development of the Haber-Weiss reaction in the focus of injury (according to Fenton chemistry) [9], which can determine the severity of thrombosis.…”

Section: Introductionmentioning

confidence: 99%

Experimental Antioxidant Biotherapy for Protection of the Vascular Wall by Modified Forms of Superoxide Dismutase and Catalase

Maksimenko¹

2005

CPD

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The antithrombotic activities of superoxide dismutase and catalase are determined by their effects on reactive oxygen species. Modification of these enzymes with chondroitin sulphate enhances the effect due to accumulation of the derivatives on the surface of the vascular wall cells. We have shown that the effects of covalently modified biocatalysts exceed those of native enzymes, free chondroitin sulphate and their mixtures. The superoxide dismutase-chondroitin sulphate conjugate markedly reduced the thrombus mass, while the catalase-chondroitin sulphate conjugate predominantly preserved blood flow. The magnitude and duration of the antithrombotic activity of modified enzymes in a rat arterial thrombosis model allows one to expect a considerable protective effect after their combined application. A single-bolus intravenous injection of the combination between superoxide dismutase-chondroitin sulphate and catalase-chondroitin sulphate covalent conjugates had a significantly lower antithrombotic effect compared with that of the superoxide dismutase-chondroitin sulphate-catalase bienzymic covalent conjugate. This could be explained by different surface distribution of the conjugates in the circulation after their intravenous administration. Biomedical study of this approach promises a new therapeutic strategy of simple and effective protection of the vascular wall against various injuries with the use of the covalent conjugate superoxide dismutase-chondroitin sulphate-catalase. The review analyses the trends of combined application of enzyme preparations to enhance the effect of antioxidant therapy and to develop conjunctive courses of thrombolytic treatment.

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Section: Introductionmentioning

confidence: 99%

Experimental Antioxidant Biotherapy for Protection of the Vascular Wall by Modified Forms of Superoxide Dismutase and Catalase

Maksimenko¹

2005

CPD

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“…Thirty-five percent ferric chloride has been used to produce arterial thrombosis (Kurz et al, 1990). It resulted in endothelial damage in the presence of flowing blood, which leads to thrombin-fibrin and platelets dependent thrombus formation (Broersma et al, 1991). The simplicity of ferric chloride induced injury has rendered this method as a prefered method for efficacy study of thrombolytic agents as well as spontaneous thrombolysis (Broersma et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Cerebral venous sinus thrombosis: Developing an experimental model

Srivastava

Gupta

Haris

et al. 2007

Journal of Neuroscience Methods

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“…Gefässsektoren verpflanzten Cooley et al [12]; ohne Heparin blieben nur 15% durchgän gig. Weitere Angaben finden wir bei Broersma et al [13] und Bevilacqua et al [ 14], und Bang et al [ 15] …”

Section: Patientinnen Und Methodenunclassified

Histologische Veränderungen in den unterbundenen Stümpfen der Ovarialgefässe bei präoperativer niedermolekularer Heparinprophylaxe

Slunsky¹

1995

Gynäkol Geburtshilfliche Rundsch

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Fragestellung: WÄhrend einer Adnexektomie untersuchten wir thrombotische VerÄnderungen sowie GefÁsslÄsionen und resezierten die StÜmpfe der Vasa ovarica nach 30 min. Methoden: Wir spritzten 2 h vor der Operation 20 mg Lovenox® s.c. Spezielle FÄrbemethoden kamen zur Anwendung. Ergebnisse: In LigaturnÄhe fanden wir 3mal (10%) in den Arterien und 4mal (13,3%) in den Venen nur wandstÄndige Thromben, die EndothelschÄden waren reduziert. Schlussfolgerungen: Niedermolekulares Heparin bietet hÖchste Wirkung und Sicherheit in der Thromboseprophylaxe. Aufgrund der Ergebnisse haben wir defÍnitiv das «alte» Standard-Heparin in der klinischen Praxis fallengelassen.

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The effect of thrombin inhibition in a rat arterial thrombosis model

Cited by 37 publications

References 9 publications

Experimental Antioxidant Biotherapy for Protection of the Vascular Wall by Modified Forms of Superoxide Dismutase and Catalase

Experimental Antioxidant Biotherapy for Protection of the Vascular Wall by Modified Forms of Superoxide Dismutase and Catalase

Cerebral venous sinus thrombosis: Developing an experimental model

Histologische Veränderungen in den unterbundenen Stümpfen der Ovarialgefässe bei präoperativer niedermolekularer Heparinprophylaxe

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