The Effect of Thiamine Deficiency on the Activity of Erythrocyte Hemolysate Transketolase

Brin, Myron; Tai, Mary; Ostashever, Alvin S.; Kalinsky, Helen

doi:10.1093/jn/71.3.273

Cited by 200 publications

(29 citation statements)

References 4 publications

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“…This background need not be fully repeated herein. Suffice it to say that the report by BRIM et al (2) in 1960 on the effect of a thiamine deficiency on the enzyme activity of a transketolase in erythrocyte hemolysates opened an enzymic methodology to determine a vitamin deficiency which was based upon the activity of an enzyme requiring the vitamin or its co enzyme form. Previous methodologies to determine vitamin deficiencies had been based upon determinations of the vitamin as a chemical substance, and the determinations were usually organic chemical, or were based upon a physical prop erty, or were by a microbiological procedure.…”

Section: Discussionmentioning

confidence: 99%

Improved and effective assays of the glutamic oxaloacetic transaminase by the coenzyme-apoenzyme system (CAS) principle.

Kishi

Folkers

1976

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryStandardized and reliable methods have been devised to assay preparations of the glutamic oxaloacetic transaminase from human erythrocytes by the CAS principle, i.e., the unsaturation and saturation of the coenzyme (pyridoxal 5'-phosphate) of a Coenzyme-Apoenzyme System. This dual enzymic assay is effective to detect and measure human deficiencies of vitamin B6. A preferred procedure has been developed, particularly for individuals and for limited groups, but is also applicable to large groups. A simplified procedure, which emphasizes the statistical significance of difference between large groups was also developed. Lyophilized erythrocyte hemolysates appear stable for up to 7 days for simplified transportation. KISHI et al. (1) recently reviewed the background of this research in connec tion with their first study of the parameters of the assay of the glutamic oxaloacetic transaminase by the CAS principle. This background need not be fully repeated herein. Suffice it to say that the report by BRIM et al. (2) in 1960 on the effect of a thiamine deficiency on the enzyme activity of a transketolase in erythrocyte hemolysates opened an enzymic methodology to determine a vitamin deficiency which was based upon the activity of an enzyme requiring the vitamin or its co enzyme form. Previous methodologies to determine vitamin deficiencies had been based upon determinations of the vitamin as a chemical substance, and the determinations were usually organic chemical, or were based upon a physical prop erty, or were by a microbiological procedure. This newer enzymic methodology for the detection and measurement of a vitamin deficiency is based upon a principle, defined by FOLKERS (3), as follows: "The specific activity of a coenzyme-apoenzyme 1 Human Deficiencies of Vitamin B6 . II. 225

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Section: Discussionmentioning

confidence: 99%

Improved and effective assays of the glutamic oxaloacetic transaminase by the coenzyme-apoenzyme system (CAS) principle.

Kishi

Folkers

1976

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…A portion of whole blood was collected by cardiac puncture using sodium citrate (3.8%) as anticoagulant. Erythrocytes were separated by centrifuging blood sampies at 1 500 rpm for 15 min at 4 oC as described by Brin et al (1960). Washed erythrocytes were diluted with an equal volume of chilled deionized distilled water and allowed to lyse.…”

Section: Tissue Collectionmentioning

confidence: 99%

“…The hexose content formed during a 60-min reaction at 38°C was determined by the anthrone method as modified by Brin et al (1960).…”

Section: Transketolase Activitymentioning

confidence: 99%

Effect of cow's milk factors on the bioavailability of non-milk thiamine in rats

Singh

Deodhar

1993

Lait

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Summary -The influence of milk factors on the bioavailability of non-milk thiamine was assessed using thiamine-deficient young rats. Twenty-eight h dialysed raw cow's milk (3.5% fat) was used as the source of milk factors. Repletion test diets provided were (A) dialysed milk alone (59 119)bound thiamine, (B) dialysed milk (59 119) plus pure thiamine (256 119) or (C) pure thiamine (315 119) as thiamine source, for 21 d. Total hepatic thiamine content, erythrocyte transketolase (ETK) activity and TPP (thiamine pyrophosphate) effect on ETK activity were selected as response criteria. Group B receiving dialysed milk supplemented with pure thiamine had significantly (P < 0.05) greater total hepatic thiamine content and ETK activity restoration than the other groups. Likewise, the TPP effect was lowest (19 ± 0.34%) in group B as compared to the other groups. The estimate of the influence of milk factors on the bioavailability of non-milk thiamine from other dietary sources after correcting for baseline data on total hepatic thiamine content and ETK activity restoration showed that bioavailability of non-milk thiamine was enhanced by > 2-fold in the presence of milk factors.

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“…A portion of whole blood was collected by cardiac puncture using sodium citrate (3.8%) as the anticoagulant. Erythrocytes were separated by centrifuging blood samples at 1 500 rpm for 15 min at 4 oC as described by Brin et al (1960).…”

Section: Tissue Collectionmentioning

confidence: 99%

“…Hexose formed during a 60-min reaction at 38 "O was estimated by the anthrone method as modified by Brin et al (1960).…”

Section: Transketolase Activity and Tpp Effect Determinationmentioning

confidence: 99%

Bioavailability of thiamine in cow milk and curd powders using rat bioassay

Singh

Deodhar

1992

Lait

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Summary -The biological availability of thiamine (relative to pure thiamine) in cow milk and curd prepared with mixed starter cultures consisting of Lectococcus lactis subsp lactis and cremoris and Propionibacterium shermanii was assessed using thiamine-depleted young rats. The cow milk and curd samples were freeze-dried. Repletion test diets provided 15~g thiamine/d from pasteurized cow milk, curd or pure thiamine for 21 d. Body weight gain, urinary thiamine excretion, hepatic thiamine content, erythrocyte transketolase (ETK) activities with and without added thiamine pyrophosphate (TPP) and TPP effect were determined as the response criteria for this purpose. Twenty-five-d thiamine depletion resulted in a significant (P < 0.01) decrease in body weight gain, decreased thiamine excretion, hepatic thiamine level, ETK activity and increased TPP effect in erythrocyte in comparison to rats receiving standard diet. Repletion with thiamine from different sources brought about a significant (P < 0.01) increase in body weight gain, urinary thiamine excretion, hepatic thiamine and ETK activity. TPP effect for erythrocytes declined significantly (P < 0.01). Restoration was more significant (P < 0.01) in the group receiving curd (CG) than that in the group receiving milk (MG), while it was higher in the MG group than the pure thiamine receiving group (PTG). It was concluded that biological availability of thiamine was greater from curd and milk than from pure thiamine.

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The Effect of Thiamine Deficiency on the Activity of Erythrocyte Hemolysate Transketolase

Cited by 200 publications

References 4 publications

Improved and effective assays of the glutamic oxaloacetic transaminase by the coenzyme-apoenzyme system (CAS) principle.

Improved and effective assays of the glutamic oxaloacetic transaminase by the coenzyme-apoenzyme system (CAS) principle.

Effect of cow's milk factors on the bioavailability of non-milk thiamine in rats

Bioavailability of thiamine in cow milk and curd powders using rat bioassay

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