The effect of the viper Cerastes cerastes cerastes venom and venom fractions on carbohydrate metabolism

Abu-Sinna, G.; Al-Zahaby, A.S.; El-Aal, A. Abd; El-Baset, A. Abd; Soliman, Nabil A.

doi:10.1016/0041-0101(93)90385-v

Cited by 12 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[23] In this study, snake venoms caused significant hypoglycemia which was observed after 2, 6, 12, and 24 h from injection of the venom. This result is in agreement with Abu-Sinna et al, [24] Al-Jammaz, [13] and Al-Sadoon et al [1] This reduction of blood glucose level reflects a disturbance in carbohydrate metabolism which could be attributed to insulin-releasing effect of some venom components.…”

Section: Resultssupporting

confidence: 92%

Toxicological Effects of Viper Cerastes Cerastes Crude Venom on Serum Indices of Male Albino Rats = التأثيرات السمية الناتجة عن السم الخام للحية المقرنة على مؤشرات مصل ذكور الجرذان البيضاء

Saleh¹,

Kasem²

2015

EJFSAT

View full text Add to dashboard Cite

Snake venoms are known to cause different metabolic and enzymatic disorders, altering cellular activities which may represent organ dysfunction. This study was conducted to examine the toxicological effects of Cerastes cerastes crude venom on serum of male albino rats through measurement of certain biochemical parameters. Materials and Methods: A total of 56 adult male albino rats (180 ± 20 g weight) were divided into two groups (28 each). In the control group, rats were received intraperitoneal (i.p.) injection with 200 µl physiological saline solution. The second group was envenomated through i.p. injection with 0.65 mg/kg b.w. of crude venom of the viper C. cerastes in 200 µl physiological saline solutions. Seven animals from the control and envenomated groups were sacrificed at 2, 6, 12 and 24 h post-injection, and the serum samples subjected for biochemical analysis. Results: The injection of C. cerastes crude venom induced a significant decrease in total serum protein, albumin, lactate dehydrogenase, glucose, cholesterol, and triglycerides at 2, 6, 12, and 24 h after injection of venom. However, the levels of total bilirubin, alanine transaminase, aspartate aminotransferase, alkaline phosphatase, gamma-glutamyl transferase, urea, and creatinine were significantly elevated in all groups of envenomated rats, while the serum level of uric acid was variable. Conclusion: The injection of viper C. cerastes crude venom caused alteration of serum biochemical parameters of albino rats with the liver and kidney dysfunction.

show abstract

Section: Resultssupporting

confidence: 92%

Toxicological Effects of Viper Cerastes Cerastes Crude Venom on Serum Indices of Male Albino Rats = التأثيرات السمية الناتجة عن السم الخام للحية المقرنة على مؤشرات مصل ذكور الجرذان البيضاء

Saleh¹,

Kasem²

2015

EJFSAT

View full text Add to dashboard Cite

show abstract

“…The different toxic effects of viper venoms are due to their proteolytic and lipolytic enzymes (Tan and Ponnudurai, 1990). Common initial signs of envenomation are hypoglycemia (Abu-Sinna et al, 1993), general metabolic disturbance (Mahmoud, 1983), muscular dystrophy (Mohamed and Khaled, 1966), nephrotoxicity (Ickowicz et al, 1966) and cytotoxicity (Bertke and Atkins, 1961).…”

Section: Discussionmentioning

confidence: 99%

Histological, molecular and biochemical detection of renal injury after Echis pyramidum snake envenomation in rats

Al-Johany

Al-Sadoon

Moneim

et al. 2015

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Nephrotoxicity is a common sign of snake envenomation. The present work aimed to clarify the effect of intraperitoneal injection of 1/8 LD50 and 1/4 LD50 doses of Echis pyramidum snake venom on the renal tissue of rats after 2, 4 and 6 h from envenomation. Histopathological examination showed intense dose and time dependent abnormalities, including swelling glomerulus and tubular necrosis and damage as well as signs of intertubular medullary hemorrhage at early stages of envenomation. However, at late stages of envenomation by any of the doses under investigation, no intact renal corpuscles were recorded and complete lysis in renal corpuscles with ruptured Bowman's capsules was observed. Immunohistochemistry by immunohistochemical staining was used to test the protein expression of Bax in renal tissue of rats. The result showed that the expression of Bax in renal tissue sections of envenomated rats was increased according to dose and time-dependant manner. The isolation of DNA from the renal cells of envenomed rats pointed out to the occurrence of DNA fragmentation, which is another indicator for renal tissue injury especially after 6 h of 1/4 LD50 of E. pyramidum envenomation. Oxidative stress biomarkers malondialdehyde and nitrite/nitrate levels, antioxidant parameters; glutathione, total antioxidant capacity and catalase were assayed in renal tissue homogenates. The venom induced significant increase in the levels of malondialdehyde and nitrite/nitrate while the levels of glutathione, total antioxidant capacity and catalase were significantly decreased, especially after 6 h of envenomation. The results revealed that the E. pyramidum induced dose and time-dependant significant disturbances in the physiological parameters in the kidney. We conclude that the use of the immunohistochemical techniques, the detection of DNA integrity and oxidative stress marker estimations are more specific tools that can clarify cellular injury and could point out to the defense activity of the renal tissue at envenomation.

show abstract

“…In addition, Mohamed et al (1980) speculate that this effect was brought about by stimulation of adrenal cortex leading to aldosterone secretion. It worthy to mention that, several studies have been made on the metabolic, cardiovascular and haematological effects of viper venoms on man and experimental animals (Tilbury et al, 1987;Soslau et al, 1988;Abu-Sinna et al, 1993;Abdul-Nabi et al, 1997 andFahim, 1998), and found that, various venoms viper cause alterations of rat metabolism (Al-Jammaz et al, 1998 and1999). Furthermore, several workers reported that, acute renal failure characterized by vascular lesions and tubular necrosis in the renal cortex following various snake bites (Tilbury et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Physiological effects of envenomation by two different doses of the viper Echis coloratusis crude venom on biochemical parameters in serum of Guinea pigs at different times.

Salman

2009

Egyptian Academic Journal of Biological Sciences. C, Physiology

View full text Add to dashboard Cite

The eastern carpet viper Echis coloratusis widely distributed from Africa (eastern Egypt). In the present study the effects of crude venom of the viper Echis coloratusis on serum biochemical parameters of guinea pigs. Adult male guinea pigs (550 ± 25 g body weigh) were divided into three groups (15 each). In the control group, guinea pigs were interaperitoneally (i.p) injected with 50 µL saline solution. The second group was i.p. injected with 0.1µgm/g b.w. of crude venom in 50 µL saline solutions. The third group was i.p. injected with 0.2 µgm/g. b.w. of the crude venom in 50 µL saline solution. The results indicated that the injection of crude venom of the viper Echis coloratusis induced a significant decrease in total serum protein, albumin, globulin and uric acid within 1, 2 and 4 hrs. after injection. In contrast, the levels of glucose, cholesterol, triglycerides, urea and creatinine were increased significantly in envenomated Guinea pigs. Viper Echis coloratusis crude venom caused hepatic and renal dysfunction in envenomated Guinea pigs.

show abstract

The effect of the viper Cerastes cerastes cerastes venom and venom fractions on carbohydrate metabolism

Cited by 12 publications

References 20 publications

Toxicological Effects of Viper Cerastes Cerastes Crude Venom on Serum Indices of Male Albino Rats = التأثيرات السمية الناتجة عن السم الخام للحية المقرنة على مؤشرات مصل ذكور الجرذان البيضاء

Toxicological Effects of Viper Cerastes Cerastes Crude Venom on Serum Indices of Male Albino Rats = التأثيرات السمية الناتجة عن السم الخام للحية المقرنة على مؤشرات مصل ذكور الجرذان البيضاء

Histological, molecular and biochemical detection of renal injury after Echis pyramidum snake envenomation in rats

Physiological effects of envenomation by two different doses of the viper Echis coloratusis crude venom on biochemical parameters in serum of Guinea pigs at different times.

Contact Info

Product

Resources

About