The effect of the use of different selective media on the ability to recover salmonellae from seagull faeces

Abstract

Solid media were compared for their ability to recover salmonellae from seagull faecal material after pre-enrichment in buffered peptone water and enrichment in Rappaport's broth. Of the 847 specimens examined 96 were found to be positive for salmonellae. Use of Brilliant Green agar containing sulphamandelate supplement resulted in the detection of salmonellae from each of the 96 samples found to be positive and was the most efficient medium tested. Brilliant Green agar lacking the supplement was the least effective medium, salmonellae being isolated from only 80 samples using this medium. All of the media tested were shown to support the growth of a wide range of salmonella serotypes, although Salmonella typhi and S. dublin did not form colonies on those media which contained Brilliant Green. Hynes' modification of deoxycholate citrate agar was shown to be considerably less inhibitory to salmonellae after ageing for four days. Ageing of other media had no significant affect on their ability to support the growth of salmonellae.

A comparison of immunomagnetic separation plus enrichment with conventional salmonella culture in the examination of raw sausages

“…The agar media above were chosen after an examination of published work and preliminary experiments which demonstrated the superior selectivity of MLCB and XLDN over the traditionally used brilliant green agar and XLD without novobiocin (Fricker and Girdwood 1984 ;Warburton et al 1994).…”

The incidence and significance of salmonella carriage by gulls (Larus spp.) in Scotland

“…The operators' gloved hands were rinsed in Clearsol 20 after processing each bird. All pre-enrichment cultures were incubated aerobically at 37°C for 24 h. Investigations to determine optimum isolation techniques for salmonellae from gull faecal material were carried out early in the study and the results of these experiments have been reported elsewhere (Fricker, Girdwood & Munro, 1983b;Fricker & Girdwood, 1984). The technique which was adopted was: pre-enrichment in BPW, enrichment in RappaportVassiliadis (RV) medium (inoculation ratio 1: 100) and plating at 24 and 48 h on brilliant green agar (Oxoid CM 329) containing sulphamandelate supplement (Watson & Walker, 1978) and Hynes's deoxycholate citrate agar (Oxoid CM 227).…”

The effect of incubation temperature and magnesium chloride concentration on growth of salmonella in home‐made and in commercially available dehydrated Rappaport‐Vassiliadis broths

“…dublin, are sometimes inhibited in media containing dyes, e.g. brilliant green (Fricker & Girdwood 1984). The possibility that the malachite green in RV could inhibit Salm.…”