The effect of the rehydration temperature and rehydration medium on the viability of freeze-dried spirillum atlanticum

Choate, Richard V.; Alexander, M. T.

doi:10.1016/s0011-2240(67)80138-x

Cited by 15 publications

(6 citation statements)

References 7 publications

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“…Furthermore, the concentration of the rehydration medium plays an important role in restoring water to the dried cell. There should be some finite rate of rehydration at which the damage to the dried cells could be minimized (Choate and Alexander 1967).…”

Section: Discussionmentioning

confidence: 99%

Screening of freeze-dried protective agents for the formulation of biocontrol strains, Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40

Yang

et al. 2011

Letters in Applied Microbiology

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Aims: The effects of different freeze‐drying protective agents on the viabilities of biocontrol strains Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40 were investigated. Method and Results: Several concentrations of protective and rehydration media were tested to improve the survival of biocontrol agents after freeze‐drying. The subsequent survival rates during storage and rehydration media of freeze‐dried biocontrol strains were also examined. Conclusions: The results indicated that cellobiose (5%) and d‐galactose (5%) gave maximum viability of strains Bu. vietnamiensis B418 and P. agglomerans 2Re40 (98 and 54·3% respectively) while the perfect one (100%) of strain B. cereus AR156 was obtained with sucrose (5%) during freeze‐drying, and the highest survival of the three strains was reached when they were rehydrated with 10% nonfat skim milk. In the following storage, the survival rates showed that B. cereus AR156 could still reach 50% after 12 months. Significance and Impact of the study: This study showed that freeze‐drying could be used to stabilize cells of these three biocontrol strains. Further studies should focus on the scale‐up possibilities and formulation development.

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Section: Discussionmentioning

confidence: 99%

Screening of freeze-dried protective agents for the formulation of biocontrol strains, Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40

Yang

et al. 2011

Letters in Applied Microbiology

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“…The concentration of the rehydration medium plays an important role in restoring water to the dried cell. There should be some finite rate of rehydration at which damage to the cells could be minimized (Choate and Alexander 1967). In the case of freeze‐dried P. agglomerans , 1% NFSM appeared to restore injured cells during rehydration, avoiding osmotic shock and, in consequence, disruption and dead cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of package and storage conditions on viability and efficacy of the freeze-dried biocontrol agent Pantoea agglomerans strain CPA-2

et al. 2002

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Aims: To reduce concentrations of protective and rehydrating media and to evaluate the effect of storage temperature, packaging and atmosphere conditions on the stability of freeze-dried Pantoea agglomerans cells. Efficacy against Penicillium digitatum of freeze-dried cells in orange fruits was also evaluated. Methods and Results: Several concentrations of protective and rehydration media were tested to reduce processing costs. Freeze-dried cells were packed in glass vials or plastic bags under vacuum or nitrogen conditions at 4 and 25°C. After 1 and 3 months, efficacy of freezedried P. agglomerans against P. digitatum was tested. Conclusions:The results indicate that it is possible to reduce the concentration of non-fat skimmed milk as a rehydration medium from 10% to 1%, maintaining viabilities of 100%. Moreover, freeze-dried cells could be stored in glass vials or in high barrier plastic bags at 4°C for 3 months while maintaining high viabilities and efficacy against P. digitatum. Significance and Impact of the Study: The major obstacle in the commercialization of biocontrol products is the development of a shelf-stable formulated product that retains biocontrol activity at a level similar to that of fresh cells. This study suggests that it is possible to maintain viability and efficacy of freeze-dried P. agglomerans cells for at least 3 months.

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“…It was not investigated if this was linked to the direct inoculation of freeze-dried cells rather than a fresh liquid culture. It has been shown that cfu counts following rehydration are highly influenced with the osmolarity of the reconstitution fluid (Choate & Alexander, 1967), the rehydration temperature (Morichi, Irie, Yano, & Kembo, 1967) as well as by the rate and volume of medium used for rehydration (De Valdez, De Giori, De Ruiz Holgado, & Olivier, 1985). Although viability losses upon inoculation occurred, the use of lyophilised cells was maintained for our assays because it is the usual procedure used in industry.…”

Section: Effect Of Starter Additionmentioning

confidence: 99%

Effect of time of inoculation, starter addition, oxygen level and salting on the viability of probiotic cultures during Cheddar cheese production

Fortin

Champagne

St‐Gelais

et al. 2011

International Dairy Journal

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The effect of the rehydration temperature and rehydration medium on the viability of freeze-dried spirillum atlanticum

Cited by 15 publications

References 7 publications

Screening of freeze-dried protective agents for the formulation of biocontrol strains, Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40

Screening of freeze-dried protective agents for the formulation of biocontrol strains, Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40

Effect of package and storage conditions on viability and efficacy of the freeze-dried biocontrol agent Pantoea agglomerans strain CPA-2

Effect of time of inoculation, starter addition, oxygen level and salting on the viability of probiotic cultures during Cheddar cheese production

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