The effect of the nimesulide and a new 4-thiazolidinone derivative on hematological parameters in the conditions of an experimental inflammatory process

Abstract

The aim of the work was to identify the anti-inflammatory effect of a newly synthesized drug from the group of 4-thiazolidinones. Thus, a comparative study of the effect of two agents was conducted: the drug Les6490 and the non-steroidal anti-inflammatory drug nimesulide. This drug belongs to the group of non-steroidal anti-inflammatory drugs (NSAIDs), which have pronounced anti-inflammatory, analgesic and antipyretic effects. Nimesulide belongs to highly selective inhibitors of cyclooxygenase and its isoenzymes (COX-2) and is used for treatment in various pathologies of the musculoskeletal system. The newly synthesized compound Les6490 belongs to the group of 4-thiazolidinone derivatives. The thiazolidinone ring is part of many existing potential antibacterial and anti-inflammatory agents, and can be combined with pyrazole fragments, which are pharmacophores of the same structure, and can contribute to increased therapeutic efficacy. The study of the peculiarities of hematological changes in rats during the experimental reproduction of the associated inflammatory process using the Freund’s adjuvant model has been conducted. As a result of the study of anti-inflammatory activity based on hematological indicators of the drugs — a new synthesized derivative of 4-thiazolidinones and a nonsteroidal anti-inflammatory drug nimesulide — their anti-inflammatory activity was revealed, which can be considered comparable to the slight advantage of the drug Les6490. At the same time, without affecting the quantitative level of erythrocytes with isolated administration, under the conditions of the inflammatory process, nimesulide and Les6490 caused a significant increase in the total number of erythrocytes. No effect on the amount of erythrocytes was found. The analysis of leukocyte formula suggests the evidence that the activity of Les6490 from the group of 4-thiazolidinones is to some extent more pronounced than the activity of nimesulide. The most pronounced changes in the leukocyte formula during AF-induced inflammation were observed on the part of neutrophils: the effect of the studied substance Les6490 was more pronounced compared to the effect of nimesulide. The introduction of the new compound Les6490 partially caused the normalization of monocytes, and under the conditions of the AF-associated inflammatory process, no significant changes were observed.

A New 4-Thiazolidinone Derivative (Les-6490) as a Gut Microbiota Modulator: Antimicrobial and Prebiotic Perspectives

“…In our previous work, we demonstrated the effect of Les-6490 on the clinical course of arthritis in rats, showing a decrease in the volume of edema of the rats' paws and a decrease in laboratory markers of inflammation, such as the erythrocyte sedimentation rate (ESR) and leukocytosis [28].…”