The Effect of the Microgravity Rotating Culture System on the Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

Wu, Xing; Li, Shaohua; Lou, Lie-ming; Chen, Zhengrong

doi:10.1007/s12033-012-9568-x

Cited by 32 publications

(14 citation statements)

References 22 publications

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“…Cell inoculation and sampling in the RCCS group were performed as described by Wu X et al [ 12 ]. Cells were harvested, and cell density was adjusted to 10 5 cells/ml.…”

Section: Methodsmentioning

confidence: 99%

“…This effect is helpful for the transport of nutrients and the removal of metabolic wastes, thus promoting chondrocyte proliferation and cartilage matrix synthesis [ 10 , 11 ]. Studies have confirmed that RCCS promotes the differentiation of BMSCs into chondrocytes and is conducive to the maintenance of the chondrocyte phenotype and biomechanical characteristics [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Transfection of the IHH gene into rabbit BMSCs in a simulated microgravity environment promotes chondrogenic differentiation and inhibits cartilage aging

Liu

et al. 2016

Oncotarget

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The effect of overexpressing the Indian hedgehog (IHH) gene on the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells (BMSCs) was investigated in a simulated microgravity environment. An adenovirus plasmid encoding the rabbit IHH gene was constructed in vitro and transfected into rabbit BMSCs. Two large groups were used: conventional cell culture and induction model group and simulated microgravity environment group. Each large group was further divided into blank control group, GFP transfection group, and IHH transfection group. During differentiation induction, the expression levels of cartilage-related and cartilage hypertrophy-related genes and proteins in each group were determined. In the conventional model, the IHH transfection group expressed high levels of cartilage-related factors (Coll2 and ANCN) at the early stage of differentiation induction and expressed high levels of cartilage hypertrophy-related factors (Coll10, annexin 5, and ALP) at the late stage. Under the simulated microgravity environment, the IHH transfection group expressed high levels of cartilage-related factors and low levels of cartilage hypertrophy-related factors at all stages of differentiation induction. Under the simulated microgravity environment, transfection of the IHH gene into BMSCs effectively promoted the generation of cartilage and inhibited cartilage aging and osteogenesis. Therefore, this technique is suitable for cartilage tissue engineering.

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“…Cell inoculation and sampling in the RCCS group were performed as described by Wu X et al [ 12 ]. Cells were harvested, and cell density was adjusted to 10 5 cells/ml.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transfection of the IHH gene into rabbit BMSCs in a simulated microgravity environment promotes chondrogenic differentiation and inhibits cartilage aging

Liu

et al. 2016

Oncotarget

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“…6 An increasing amount of research is focused on the effects of gravity alterations on the physiological processes, but also on the possibility to exploit this stimulus as a potential therapeutic cue. [7][8][9] As an example, improved regeneration of infarcted myocardium has been achieved after injection of stem cells differentiated following …”

Section: Introductionmentioning

confidence: 99%

Barium titanate nanoparticles and hypergravity stimulation improve differentiation of mesenchymal stem cells into osteoblasts

Rocca¹,

Marino²,

Rocca³

et al. 2015

IJN

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Background Enhancement of the osteogenic potential of mesenchymal stem cells (MSCs) is highly desirable in the field of bone regeneration. This paper proposes a new approach for the improvement of osteogenesis combining hypergravity with osteoinductive nanoparticles (NPs). Materials and methods In this study, we aimed to investigate the combined effects of hypergravity and barium titanate NPs (BTNPs) on the osteogenic differentiation of rat MSCs, and the hypergravity effects on NP internalization. To obtain the hypergravity condition, we used a large-diameter centrifuge in the presence of a BTNP-doped culture medium. We analyzed cell morphology and NP internalization with immunofluorescent staining and coherent anti-Stokes Raman scattering, respectively. Moreover, cell differentiation was evaluated both at the gene level with quantitative real-time reverse-transcription polymerase chain reaction and at the protein level with Western blotting. Results Following a 20 g treatment, we found alterations in cytoskeleton conformation, cellular shape and morphology, as well as a significant increment of expression of osteoblastic markers both at the gene and protein levels, jointly pointing to a substantial increment of NP uptake. Taken together, our findings suggest a synergistic effect of hypergravity and BTNPs in the enhancement of the osteogenic differentiation of MSCs. Conclusion The obtained results could become useful in the design of new approaches in bone-tissue engineering, as well as for in vitro drug-delivery strategies where an increment of nanocarrier internalization could result in a higher drug uptake by cell and/or tissue constructs.

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“…This indicates that the p38 MAPK signal mediates s- μg -induced chondrogenesis of ADSCs. In MSCs cultured during chondrogenic induction in a rotating culture, combined with polyglycolic acid (PGA), mRNA and proteins of collagen type II and aggrecan were significantly more expressed in the s- μg rotating culture group than the static culture group, as reported by Wu et al [25]. Zhang et al [26] described that MSCs spread out in a spindle shape when cultured in normal gravity, while they become unspread and round under s- μg .…”

Section: Mesenchymal Stem Cells and Microgravitymentioning

confidence: 83%

The Impact of Simulated and Real Microgravity on Bone Cells and Mesenchymal Stem Cells

Ulbrich

Wehland

Pietsch

et al. 2014

BioMed Research International

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How microgravity affects the biology of human cells and the formation of 3D cell cultures in real and simulated microgravity (r- and s-µg) is currently a hot topic in biomedicine. In r- and s-µg, various cell types were found to form 3D structures. This review will focus on the current knowledge of tissue engineering in space and on Earth using systems such as the random positioning machine (RPM), the 2D-clinostat, or the NASA-developed rotating wall vessel bioreactor (RWV) to create tissue from bone, tumor, and mesenchymal stem cells. To understand the development of 3D structures, in vitro experiments using s-µg devices can provide valuable information about modulations in signal-transduction, cell adhesion, or extracellular matrix induced by altered gravity conditions. These systems also facilitate the analysis of the impact of growth factors, hormones, or drugs on these tissue-like constructs. Progress has been made in bone tissue engineering using the RWV, and multicellular tumor spheroids (MCTS), formed in both r- and s-µg, have been reported and were analyzed in depth. Currently, these MCTS are available for drug testing and proteomic investigations. This review provides an overview of the influence of µg on the aforementioned cells and an outlook for future perspectives in tissue engineering.

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The Effect of the Microgravity Rotating Culture System on the Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

Cited by 32 publications

References 22 publications

Transfection of the IHH gene into rabbit BMSCs in a simulated microgravity environment promotes chondrogenic differentiation and inhibits cartilage aging

Transfection of the IHH gene into rabbit BMSCs in a simulated microgravity environment promotes chondrogenic differentiation and inhibits cartilage aging

Barium titanate nanoparticles and hypergravity stimulation improve differentiation of mesenchymal stem cells into osteoblasts

The Impact of Simulated and Real Microgravity on Bone Cells and Mesenchymal Stem Cells

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