The Effect of the Hot Water Extracts of the <i>Paecilomyces hepiali </i>and <i>Cordyceps militaris</i> Mycelia on the Growth of Gastrointestinal Bacteria

Abstract

The gastrointestinal health is very important aspect concerning human health. It refers to nutrient and vitamin absorption, food digestion and various infectious diseases. The Paecilomyces hepiali and Cordyceps militaris are highly enriched with cordycepin and ergosterol which are considered as anti bacterial substances. Present study finds out comparative effect of hot water extract of particular fungal material on growth of six species of gastrointestinal bacteria that belong to both aerobic and anaerobic and, consist with harmful and commensal categories. The appropriate concentration level of hot water extract of both strains was identified. The individual specific bacterial growing media were prepared and calculated; amounts of bacteria cultures were inoculated by using micro pipettes. The optical density and number of bacterial colonies were measured after 24 hours. The pure mycelial extract of P. hepiali with 2 × 10 −3 g/ml of concentration has significant effect on depleting the growth of E. coli, E. faecalis, S. aureus, L. gasseri and B. ovatus bacteria. B. longum has no significant effect by particular extract. Same type of extract of C. militaris has significantly reduced the growth of every bacteria used in this study. Hot water extract of C. militaris cultivated on soy bean has significant growth retardation toward E. coli, E. faecalis, S. aureus and L. gasseri. It has stimulated the growth of B. ovatus and B. longum which are considered as beneficial bacteria for human gut. This study shows that extracts of both mycelia include antimicrobial substances like cordycepin and ergosterol which can be used as food supplements to enhance human gut health.

The Quality and Functional Improvement of Retorted Korean Ginseng Chicken Soup (Samgyetang) by Enzymolysis Pre-Treatment with Cordyceps militaris Mushroom Extract

“…The greatest contributor for taste-related compounds from the CM mushroom are believed to be from the 5'-AMP, soluble sugars, free amino acids, and volatile compounds [56]. In addition, the abundant monosodium glutamate-like amino acid in the CM mushroom [57] may have also contributed to the umami taste, as was also found in our previous study. Cordyceps militaris mushroom extract: bromelain:flavorzyme before retorting.…”

Investigation of Taste-Related Compounds and Antioxidative Profiles of Retorted Samgyetang Made from Fresh and Dried Cordyceps militaris Mushrooms

“…The expected mechanism is that exogenous AMP, which penetrates into the meat muscle was not completely converted into IMP and remain unchanged from a state of AMP residue. AMP is one of the major bioactive compounds within the C. militaris mushroom with a broad range of pharmacological functions ( Gamage et al, 2018 ). Through adenosine monophosphate deaminase (AMPD) pathway, AMP could be converted into ammonia and IMP with the rate of convertion is depend on various factors including heat ( Wang et al, 2016 ).…”

Interplay between Fungal Infection and Bacterial Associates in the Wax Moth Galleria mellonella under Different Temperature Conditions

“…For example, significant changes in the mouse gut microbiome were observed after feeding mice a major metabolite of C. militaris cordycepin [ 76 ]. Gamage and coworkers [ 77 ] showed that С. militaris water extracts exhibited different levels of inhibition of various gram-positive and gram-negative bacteria.…”