The effect of the gene carrier material polyethyleneimine on the structure and function of human red blood cells in vitro

Li, Chenghua; Zhong, Dagen; Zhang, Yi; Tuo, Wei-Wei; Li, Nan; Wang, Qian; Liu, Zonghua; Wang, Xue

doi:10.1039/c3tb00024a

Cited by 26 publications

(31 citation statements)

References 29 publications

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“…RBCs are one of the most abundant components of mammalian blood, comprising about 40–50% of the volume fraction of whole blood. Normally, RBCs have a biconcave disk shape; however, that can easily change when they interact with foreign substances . In this work, RBC morphology was observed after exposure to different concentrations of mPEG–PCL solution [Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Hemolysis, which indicates the disturbed integrity of the RBC membrane structure, is measured by the release of intracellular hemoglobin. The hemolysis assay is extensively used in vitro to examine the biocompatibility of biomedical materials . In this work, the hemolysis assay was conducted with different concentrations of mPEG2k–PCL2k and mPEG5k–PCL2k [Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Normally, RBCs have a biconcave disk shape; however, that can easily change when they interact with foreign substances. 22 In this work, RBC morphology was observed after exposure to different concentrations of mPEG-PCL solution [ Fig. 4(a)].…”

Section: Interactions Between Mpeg-pcl and Rbcsmentioning

confidence: 99%

“…The hemolysis assay is extensively used in vitro to examine the biocompatibility of biomedical materials. 22 In this work, the hemolysis assay was conducted with different concentrations of mPEG2k-PCL2k and mPEG5k-PCL2k [ Fig. 4(b,c)].…”

Section: Interactions Between Mpeg-pcl and Rbcsmentioning

confidence: 99%

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Hemocompatibility evaluation in vitro of methoxy polyethyleneglycol–polycaprolactone copolymer solutions

Zhang

Wang

et al. 2016

J Biomedical Materials Res

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Amphiphilic block copolymer methoxy polyethyleneglycol-polycaprolactone (mPEG-PCL) has attracted interest in the biomedical field, due to its water solubility and biodegradability. Nevertheless, the blood safety of mPEG-PCL copolymers has not been investigated in detail. Because mPEG-PCL copolymers introduced in vivo would inevitably interact with blood tissue, an investigation of possible interactions of mPEG-PCL with key blood components is crucial. We studied the effects of two mPEG-PCL copolymer solutions on blood coagulation, the morphology and lysis of human red blood cells (RBCs), the structure of plasma fibrinogen, complement activation, and platelet aggregation. We found that higher concentrations of the mPEG-PCL copolymers impaired blood clotting, and the copolymers had little impact on the morphology or lysis of RBCs. From the spectroscopy results, the copolymers affected the local microstructure of fibrinogen. The copolymers significantly activated the complement system in a concentration-dependent way. At higher concentrations, the copolymers impaired platelet aggregation, which may have been mediated by an inhibition of the arachidonic acid pathway. These findings provide important information that may be useful for the molecular design and biomedical applications of mPEG-PCL copolymers. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 802-812, 2016.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Interactions Between Mpeg-pcl and Rbcsmentioning

confidence: 99%

Section: Interactions Between Mpeg-pcl and Rbcsmentioning

confidence: 99%

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Hemocompatibility evaluation in vitro of methoxy polyethyleneglycol–polycaprolactone copolymer solutions

Zhang

Wang

et al. 2016

J Biomedical Materials Res

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“…All samples were incubated for 12 h or 24 h at 37°C. After the incubation, the RBC suspensions were centrifuged at 10009g for 5 min, and the supernatants were measured for the absorbance at 540 nm by using a microplate reader [18]. The percentage hemolysis was calculated by measuring the optical density (OD) as the following formula:…”

Section: Hemolysismentioning

confidence: 99%

Redox poly(ethylene glycol)-b-poly(l-lactide) micelles containing diselenide bonds for effective drug delivery

Zeng

Zhou

et al. 2015

J Mater Sci: Mater Med

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Bioreducible polymers have appeared as the ideal drug carriers for tumor therapy due to their properties of high stability in extracellular circulation and rapid drug release in intracellular reducing environment. Recently, the diselenide bond has emerged as a new reduction-sensitive linkage. In this work, the amphiphilic poly(ethylene glycol)-b-poly(L-lactide) containing diselenide bond has been synthesized and used to load anti-tumor drug, docetaxel (DTX), to form the redox micelles. It was found that the redox micelles showed a rapid response to glutataione (GSH), which resulted in a fast release of DTX in the presence of GSH. In contrast, <40 % of DTX was released from the micelles within 72 h under the normal condition (absence of GSH). The DTX-loaded redox micelles showed the significant inhibition effect to MCF-7 cells, and the cytotoxicity was dependent on the intracellular GSH concentrations. Moreover, considering the potentially clinical applications of the micelles through intravenous injection, the blood compatibility was also studied by the hemolysis analysis, activated partial thromboplastin time, prothrombin time and thromboelastography assays. These results confirmed that the redox micelles showed good blood safety, suggesting a potential application in tumor therapy.

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Recent Advances in Engineering Carriers for siRNA Delivery

Yang,

Lin,

Zhang

et al. 2024

Macromolecular Bioscience

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RNA interference (RNAi) technology has been a promising treatment strategy for combating intractable diseases. However, the applications of RNAi in clinical were hampered by extracellular and intracellular barriers. To overcome these barriers, various siRNA delivery systems have been developed in the past two decades. The first approved RNAi therapeutic, Patisiran (ONPATTRO™) using lipids as the carrier, for the treatment of amyloidosis is one of the most important milestones. This has greatly encouraged researchers to work on creating new functional siRNA carriers. In this review, we summarized the recent advances in siRNA carriers consisting of lipids, polymers, and polymer‐modified inorganic particles for cancer therapy. Representative examples are presented to show the structural design of the carriers in order to overcome the delivery hurdles associated with RNAi therapies. Finally, the existing challenges and future perspective for developing RNAi as a clinical modality will be discussed and proposed. We believe that the addressed contributions in this review will promote the development of siRNA delivery systems for future clinical applications.This article is protected by copyright. All rights reserved

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The effect of the gene carrier material polyethyleneimine on the structure and function of human red blood cells in vitro

Cited by 26 publications

References 29 publications

Hemocompatibility evaluation in vitro of methoxy polyethyleneglycol–polycaprolactone copolymer solutions

Hemocompatibility evaluation in vitro of methoxy polyethyleneglycol–polycaprolactone copolymer solutions

Redox poly(ethylene glycol)-b-poly(l-lactide) micelles containing diselenide bonds for effective drug delivery

Recent Advances in Engineering Carriers for siRNA Delivery

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