The effect of the degree of chitosan deacetylation on the efficiency of gene transfection

Kiang, Tina; Wen, Jie; Lim, Huay Wen; Leong, Kam W.

doi:10.1016/j.biomaterials.2003.12.036

Cited by 318 publications

(224 citation statements)

References 30 publications

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“…These results were the same manner with [32] who indicated that keeping the DNA concentration constant and changing of chitosan concentration. DNA binding is dependent on both molecular weight and the degree of deacetylation of the used chitosan, which proved that particle size and shape play an important role in transferring genes into the cells according to [40].…”

Section: Determination Of Degree Of Deacetylation (Dd)supporting

confidence: 84%

“…The imaging of CS/pDNA complex under TEM agreed with [32] who photographed chitosan with pcDNA containing human embryonic kidney cells (HEK293); and [41] who photographed pUMVC3-hIL12 loaded chitosan nanoparticles in Colon Carcinoma Cells. Up till now few researches included application of chitosan in plant and the work in this field is too narrow, [33] who imaged the pBDG loaded to chitosan complex which transfected in both Echinacea purpurea and Paulownia sp plants respectively.…”

Section: Transmission Electron Microscope (Tem)supporting

confidence: 74%

“…Transmission electron microscopy According to [32] the method for TEM imaging as follow: carbon coated 400 mesh copper grids were put over one drop of the complex (CS/pDNA) and left to stand for 1.5 min. The grid was stained with one drop of filtered solution containing 2% uranyl acetate for 1.5 min, and any excess uranyl acetate was removed with filter paper.…”

Section: 64mentioning

confidence: 99%

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Transformation of Thionin Genes Using Chitosan Nanoparticle into Potato Plant to Be Resistant to Fungal Infection

Abdel-Razik¹,

Hammad²,

Tawfik³

2017

IOSR JBB

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Section: Determination Of Degree Of Deacetylation (Dd)supporting

confidence: 84%

Section: Transmission Electron Microscope (Tem)supporting

confidence: 74%

Section: 64mentioning

confidence: 99%

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Transformation of Thionin Genes Using Chitosan Nanoparticle into Potato Plant to Be Resistant to Fungal Infection

Abdel-Razik¹,

Hammad²,

Tawfik³

2017

IOSR JBB

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“…The positive surface charge of CS in acidic medium due to protonation of the amine groups in chitosan showed repulsive forces (Kiang et al, 2004) between nanoparticles, which support our findings. …”

Section: Zeta Potentialsupporting

confidence: 89%

“…The decrease in zeta potential therefore follows the reduction in the protonation as pH increases. The positive surface charge of CS in acidic medium due to protonation of the amine groups in CS showed repulsive forces (Kiang, Wen, Lim, & Leong, 2004) between nanoparticles, which support these findings. The large amount of drug accumulated on the particle surface resulted in reduced surface positive charge favouring agglomeration.…”

Section: Zeta Potentialsupporting

confidence: 73%

Development of Nicotine Loaded Chitosan Nanoparticles for Lung Delivery

Wang¹

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Cigarette smoking has become one of the leading causes of preventable deaths all over the world, causing a serious threat to human wellbeing and a tremendous economic burden to governments. The currently available treatment options for smoking cessation are not efficient. The pulmonary delivery of drugs by methods such as dry powder inhaler (DPI) has been recognized as one of the most efficient routes of drug delivery to the targeted area. The lung delivery of nicotine in this way would be expected to mimic the effects of tobacco smoking and could significantly reduce the negative health effects of smoking that result from nicotine addiction.The aim of this study is to develop nanoparticles with controlled physicochemical properties using biodegradable polymer of chitosan (CS) loaded with nicotine salt for pulmonary delivery as DPI formulations. The outcomes of this project are expected to be a safe and effective CS-based nicotine formulation for pulmonary delivery to treat nicotine dependence. This thesis specially addresses the research questions: (1) how to develop a feasible process to manufacture the nanoparticles suitable for pulmonary drug delivery using biodegradable polymer of CS containing nicotine salt; (2) how to develop an animal model for pulmonary drug delivery from DPI formulation using a nose-only inhalation device.The current therapeutic options for treatment of smoking addiction have been reviewed, and current advancements of technology in pulmonary drug delivery are summarised. Buccal administration of drugs exhibits a low oral bioavailability, and sublingual tablets and chewing gums can be swallowed before being absorbed.Although nasal spray of nicotine is a fast way to deliver nicotine into the bloodstream, Development of Nicotine Loaded Chitosan Nanoparticles for Lung Delivery iii the rate of absorption is still not as rapid as cigarette smoking. Therefore, it is proposed that delivery of nicotine with sustained drug release profile direct into the deep lung is likely to more effectively mimic the rapid effects of cigarette smoking on a physiological level, which could eliminate patient craving and allow the tapering of nicotine level over time to improve patients' compliance.Water in oil (w/o) emulsion crosslinking method has been used to fabricate nicotine hydrogen tartrate (NHT)-loaded CS nanoparticles which separate as solid microaggregates. The physicochemical properties of particles are characterized by a series of techniques. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) shows that the prepared particles are spherical in morphology with rough surface after loading CS particles with NHT. Dynamic Light Scattering (DLS)by Zetasizer determined nanoparticle size ranging from 167.6 nm to 411 nm, while DLS by Mastersizer demonstrated that the microaggregates of these nanoparticles are in the respirable range (<5µm). The surface positive charge as measured by zeta potential tends to decrease with increase of mass ratios of NHT to CS, which is in contr...

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Natural Polymeric Vectors in Gene Therapy

Kundu

2011

Biopolymers

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The effect of the degree of chitosan deacetylation on the efficiency of gene transfection

Cited by 318 publications

References 30 publications

Transformation of Thionin Genes Using Chitosan Nanoparticle into Potato Plant to Be Resistant to Fungal Infection

Transformation of Thionin Genes Using Chitosan Nanoparticle into Potato Plant to Be Resistant to Fungal Infection

Development of Nicotine Loaded Chitosan Nanoparticles for Lung Delivery

Natural Polymeric Vectors in Gene Therapy

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