The effect of the chelator characteristics on insulin adsorption in immobilized metal affinity chromatography

Erol, Beray; Erol, Kadir; Gökmeşe, Ebru

doi:10.1016/j.procbio.2019.05.009

Cited by 32 publications

(14 citation statements)

References 40 publications

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“…High specificity [12] Powerful tool for analyzing membrane proteins, histidine-tagged proteins, and phosphorylated proteins by using single-step purification [12] procedures [12] easy preparation [13] high adsorption capacity [13], high stability [13] Combined with MALDI-TOF MS detection, high sensitivity detection is achieved [14] The coordination can be reversed by separating the bound proteins using competing ligands (eg imidazole) [15] Glycopeptides and phosphopeptides can be captured simultaneously, by combining with other mechanisms such as HILIC, MOAC [9] No biospecific interaction [16] Many proteins do not have metal affinity [16] Destabilization of purified protein solution due to metal ions [16] Care should be taken when using chelating agents such as EDTA, as it can strip metal ions off the surface [17] Metal oxide affinity chromatography (MOAC)…”

Section: Techniquesmentioning

confidence: 99%

“…Here, the basic interactions between the target biomolecule and the affinity sorbent are also listed for each chromatographic method. The most common advantages and disadvantages of these methods are comparatively documented in Table 1 [9,12–41]. The information presented in Figure 2 and Table 1 can be used as a starting point for the selection of an appropriate chromatographic method for a certain biomolecule that is isolated or enriched from a complex biological sample.…”

Section: Bioaffinity Chromatography Techniquesmentioning

confidence: 99%

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Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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Isolation or enrichment of biological molecules from complex biological samples is mostly a prerequisite in proteomics, genomics, and glycomics. Different techniques have been used to advance the efficiency of the purification of biological molecules. Bioaffinity chromatography is one of the most powerful technique that plays an important role in the isolation of target biological molecules by the specific interactions with ligands that are immobilized on different support materials. This review examines the recent developments in bioaffinity chromatography particularly over the past 5 years in the literature. Also properties of supports, immobilization techniques, types of binding agents, and methods used in bioaffinity chromatography applications are summarized.

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Section: Techniquesmentioning

confidence: 99%

Section: Bioaffinity Chromatography Techniquesmentioning

confidence: 99%

Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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“…Langmuir model specifically describes the homogeneous and monolayer adsorption on fixed adsorption sites (Kireç et al 2021). Freundlich model assumes the heterogeneous multilayer adsorption on different surface energies (Erol et al 2019a(Erol et al , 2019b Meanwhile, Temkin isotherm model describes that the heat of adsorption decreases with the adsorption coverage due to the adsorbent-adsorbate interactions (Bahrudin et al 2019). The non-linear expressions of Langmuir (Langmuir 1918), Freundlich (Freundlich 1906), and Temkin (Temkin 1940) isotherms are given in Equations ( 7)-( 9), respectively as follows:…”

Section: Isotherm Modelingmentioning

confidence: 99%

Microporous activated carbon developed from KOH activated biomass waste: surface mechanistic study of methylene blue dye adsorption

Jawad

Abdulhameed

Bahrudin

et al. 2021

Water Science and Technology

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In this work, sugarcane bagasse waste (SBW) was used as a lignocellulosic precursor to develop a high surface area activated carbon (AC) by thermal treatment of the SBW impregnated with KOH. This sugarcane bagasse waste activated carbon (SBWAC) was characterized by means of crystallinity, porosity, surface morphology and functional groups availability. The SBWAC exhibited Type I isotherm which corresponds to microporosity with high specific surface area of 709.3 m2/g and 6.6 nm of mean pore diameter. Further application of SBWAC as an adsorbent for methylene blue (MB) dye removal demonstrated that the adsorption process closely followed the pseudo-second order kinetic and Freundlich isotherm models. On the other hand, thermodynamic study revealed the endothermic nature and spontaneity of MB dye adsorption on SBWAC with high acquired adsorption capacity (136.5 mg/g). The MB dye adsorption onto SBWAC possibly involved electrostatic interaction, H-bonding and π-π interaction. This work demonstrates SBW as a potential lignocellulosic precursor to produce high surface area AC that can potentially remove more cationic dyes from the aqueous environment.

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“…Magnitude of the magnetic field and the temperature of the material produce diverse spin orientations. The g factor, the spectroscopic cleavage, is calculated according to the following equation [41].…”

Section: Characterization Of the Adsorbentsmentioning

confidence: 99%

Amine Modified Magnetic Diatomite Particles as an Effcicent Adsorbent for Pecticide Removal

Alacabey¹

2021

Preprint

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Pesticides are one of the most critical emerging contaminants which are highly toxic for the environment and have potential risk to human health. In this study, surface-modified magnetic diatomite particles (m-DE-APTES) have been suc-cessfully synthesized and used as a sorbent for the removal of endosulfan from an aqueous solution. Magnetic diatomite particles with surface modification were characterized by Fourier transform-infrared spectroscopy (FT-IR), scan-ning electron microscopy (SEM), energy dispersive X-ray (EDX), electron spin resonance (ESR), and surface area measurements. Characterization results sug-gest that magnetic diatomite has a high surface area and porous structure. In addition, m-DE-APTES has higher adsorption capacity (97.2 mg/g) for en-dosulfan pesticide than unmodified diatomite particles (DE) (16.6 mg/g). The adsorption data fit the Langmuir model (R2=0.9905), and the adsorption process took place spontaneously with the values of ΔGo as -2.576. In conclusion, the surface-modified diatomite particles are promising alternative adsorbents for pesticide removal from aqueous solutions.

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The effect of the chelator characteristics on insulin adsorption in immobilized metal affinity chromatography

Cited by 32 publications

References 40 publications

Recent trends in sorbents for bioaffinity chromatography

Recent trends in sorbents for bioaffinity chromatography

Microporous activated carbon developed from KOH activated biomass waste: surface mechanistic study of methylene blue dye adsorption

Amine Modified Magnetic Diatomite Particles as an Effcicent Adsorbent for Pecticide Removal

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