The effect of the aflatoxins B1, G1, and G2 on protein and nucleic acid synthesis in rat liver

Clifford, JI; Rees, K. R.; Stevens, M.

doi:10.1042/bj1030258

Cited by 69 publications

(10 citation statements)

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“…Thus, inhibition of RNA synthesis is accompanied by nucleolar segregation (Svoboda and Higginson, 1968), and inhibition of protein synthesis by the detachment of ribosomes from the rough-surfaced endoplasmic reticulum and the proliferation of smooth membranes (Butler, 1966;Clifford, Rees and Stevens, 1967;Svoboda and Higginson, 1968;Harley, Rees and Cohen, 1969). Apparent membrane degranulation is often seen as a response to drug challenge and accompanies the induction of enzymes associated with nascent smooth membrane and is also associated with drug detoxication (Arcos, Conney and Buu-Hoi, 1961;Orrenius and Ericsson, 1966;Conney, 1967).…”

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confidence: 99%

The Effects of Aflatoxin B1 in vivo on Membrane—Ribosome Association

Williams¹,

Clark²,

Rabin³

1973

Br J Cancer

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Summary.-The effects of aflatoxin B1 on the endoplasmic reticulum of rat liver has been examined in vivo. Electron microscopy has shown a disorganization and degranulation of rough surfaced membrane under these conditions and evidence is presented that this is a primary effect of the toxin, and results from the direct attack of the aflatoxin on the steroid-dependent ribosome binding sites on the membrane. A technique is described by which the presence of degranulated rough membrane may be detected in microsomal preparations.

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confidence: 99%

The Effects of Aflatoxin B1 in vivo on Membrane—Ribosome Association

Williams¹,

Clark²,

Rabin³

1973

Br J Cancer

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“…Experiments pertaining to the role of aflatoxin as a cytotoxin were done on biological systems. The mode of action of aflatoxin in the molecular level was largely revealed by the work of Clifford and Rees (1967). They studied the effect of AFB 1 on DNA replication, transcription of messenger RNA or translation of m-RNA into protein.…”

Section: Discussionmentioning

confidence: 99%

Effect of detoxified Aflatoxin B1 contaminated products on some in vivo and in vitro plant physiological systems

Das

Mishra

2001

Acta Physiol Plant

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Aflatoxin BI was detoxified (60 %) enzymatically by horseradish peroxidase. In another set of experiments the toxin was detoxified (97 %) by a combination of enzyme and microwave treatments. Chloroform extracts of these reaction products were used to study their effects on some important physiological processes of higher plants. Cell mass growth in suspension culture of Catharanthus roseus showed 34 % and 77 % respective increase in dry weight after 4 to 8 weeks of incubation at room temperature (22 EC) without toxin but in presence of toxin only 21% and 43 % dry weight increase occurred in the same time interval. Aflatoxin B 1 showed a profound effect on pollen germination and pollen tube morphology leading to only 40 % germination and several morphological anomalies in Catharanthus roseus and Haemanthus Katherinae. Embryogenic callus of Santalum album could give rise to only 60 % somatic embryo in presence of lmM toxin with several abnormalities which is much less compared to 94 % conversion to distinct bipolar embryos in case of control set without toxin. Only 49 % seeds ofArachis hypogaea germinated in presence of 1 mM aflatoxin in the germinating medium compared to 71 % germination in control media.

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“…At the molecular level aflatoxins impart their effect either interfering with DNA replication or transcription of messenger RNA into protein. Clifford and Rees (1967) revealed that the administration of a single dose of aflatoxin (7 mg/kg body weight) resulted in slow development of periportal necrosis. Hepatic enzymes were released into the serum after 48 h of poisoning.…”

Section: Structure and Function Of Aflatoxin Moleculesmentioning

confidence: 98%

A Review on Biological Control and Metabolism of Aflatoxin

Mishra¹,

Das²

2003

Critical Reviews in Food Science and Nutrition

249

135

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The series of events that led to the discovery of aflatoxin as a potent carcinogen, its biosynthesis, mechanism of action, structure-function relationship provide interesting insight into the economical and technological factors involved in the development of an effective control measure for the toxin. Scientists all over the world are making continuous efforts to explore a generalized process of detoxification, which can bring down the toxin content in heterogenous commodities to a threshold level. In this article biological control methods with special emphasis on in vivo and in vitro enzymatic detoxification of aflatoxin have been reviewed. Future areas of research involving large-scale enzymatic detoxification and modified atmosphere storage are also discussed.

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The effect of the aflatoxins B1, G1, and G2 on protein and nucleic acid synthesis in rat liver

Cited by 69 publications

References 15 publications

The Effects of Aflatoxin B1 in vivo on Membrane—Ribosome Association

The Effects of Aflatoxin B1 in vivo on Membrane—Ribosome Association

Effect of detoxified Aflatoxin B1 contaminated products on some in vivo and in vitro plant physiological systems

A Review on Biological Control and Metabolism of Aflatoxin

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