The method of rotating bending fatigue test under an electrochemical hydrogen charging was established, by which the fatigue tests were carried out for a JIS SCM435 to study the effect of hydrogen on fatigue strength. The fatigue life of the specimen was degraded by hydrogen charging in relatively low fatigue life regime, where the slope of S N curve for the hydrogen charged specimens was equivalent to that for no charged specimens. The fatigue strength on 50 failure probability at 10 7 cycles under the hydrogen charging was slightly lower than that under the hydrogen free condition. On the surface of the non charged specimen that was run out at 10 7 cycles, a number of cracks up to ≈ 100 µm in length was observed. By contrast, in the hydrogen charged specimen, no crack was observed after the fatigue testing. These results suggested that the hydrogen charging had a negative in uence on the fatigue limit as well as fatigue life of the alloy used in this study.