The effect of temperature on hairy root cultures of Catharanthus roseus: Growth, indole alkaloid accumulation and membrane lipid composition

Toivonen, Leena; Laakso, Simo; Rosenqvist, Heikki

doi:10.1007/bf00234368

Cited by 21 publications

(20 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The reason for the composition of fatty acids being restricted to these few major species may be that the lipid classes in rapidly growing plant cells are predominantly polar lipids, namely phospholipids and glycolipids, which are the main membrane lipids (MacCarthy & Stumpf 1980). The fatty acid composition in our transformed roots resembled that recently found in the hairy roots of Catharanthus roseus (Toivonen et al 1992). However, the relative amounts of fatty acids differed considerably, especially in the case of linoleic and oleic acids, the latter being one of the major components in C. roseus (over 25 %).…”

Section: Lipid Composition Of the Transformed Root Clonesmentioning

confidence: 51%

See 1 more Smart Citation

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

Oksman-Caldentey

Sev�n

Vanhala

et al. 1994

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The effect of carbon and nitrogen sources on two well-established hairy root clones, LBA1S and C58A, of Hyoscyamus muticus strain Cairo, were investigated. Both clones exhibited completely different patterns with regards to their growth rate, hyoscyamine accumulation, and fatty acid contents. Clone C58A grew faster and yielded more biomass (17.4 g 1-1, in 21 days), but produced less hyoscyamine. The maximum hyoscyamine content (120 mg 1-1) in clone LBA1S was reached in 28 days. Neither of the clones could use lactose or fructose as the sole carbon source, nor ammonium as the sole nitrogen source. The growth in the medium containing glucose was significantly reduced compared to that containing sucrose. Clone LBA1S was sensitive to the changes in sucrose concentration and an increase in ammonium in the culture medium, whereas C58A tolerated these changes better but was more sensitive to the increase in total nitrogen. Lipid synthesis was active in the exponential growth phase, and the total fatty acid content varied from 5 to 34 mg g-I of dry root material. The major fatty acids were linoleic, palmitic and linolenic. There were considerable differences in the total amount of lipids and in their relative ratios when different nutrients were applied.Abbreviations: DW --dry weight, FA --fatty acids, FFA--free fatty acids, FW --fresh weight

show abstract

Section: Lipid Composition Of the Transformed Root Clonesmentioning

confidence: 51%

“…Radwan et al 1979; Barz et al 1980), but very little is known about the lipid composition in transformed root cultures (Toivonen et al 1992). Temperature alteration as well as other environmental stresses or changes in nutrient composition may induce modifications in lipid composition (Weber & Marigold 1988).…”

Section: Introductionmentioning

confidence: 99%

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

Oksman-Caldentey

Sev�n

Vanhala

et al. 1994

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Toivonen et al (1992) showed that a high temperature influenced the growth rate and the indole alkaloid content in the roots of transformed C. roseus, due to modification of the lipid content of the cell membrane. Lowering of the temperature to 19.5°C resulted in a higher degree of fatty acids unsaturation mainly through the regulation of linolic and linolenic acids accumulation.…”

Section: Influence Of Ph and Temperaturementioning

confidence: 99%

Catharanthus roseus: micropropagation and in vitro techniques

Pietrosiuk

Furmanowa

Łata³

2007

Phytochem Rev

View full text Add to dashboard Cite

Different methods of in vitro culture of Catharanthus roseus provide new sources of plant material for the production of secondary metabolites such as indole alkaloids. Callus, cell suspension, plantlets, and transgenic roots cultured in the bioreactor are used in those experiments.The most promising outcomes include the production of the following indole alkaloids: ajmalicine in unorganised tissue, catharanthine in the leaf and cell culture in the shake flask and airlift bioreactor, and vinblastine in shoots and transformed roots. What is very important, enzymatic coupling of monomeric indole alkaloids, vindoline and catharanthine, is possible to form vinblastine in cell cultures. The method of catharanthine and ajmalicine production in the suspension culture in bioreactors has been successful. In this method, elicitation may be used acting on different metabolic pathways. Also of interest is the method of obtaining arbutin from the callus culture of C. roseus conducted with hydroquinone. The transformed root culture seems to be the most promising for alkaloid production. The genetically transformed roots, obtained by the infection with Agrobacterium rhizogenes, produce higher levels of secondary metabolites than intact plants. Also, whole plants can be regenerated from hairy roots. The content of indole alkaloids in the transformed roots was similar or even higher when compared to the amounts measured in studies of natural roots. The predominant alkaloids in transformed roots are ajmalicine, serpentine, vindoline and catharanthine, found in higher amounts than in untransformed roots. Transformed hairy roots have been also used for encapsulation in calcium alginate to form artificial seeds.

show abstract

“…The establishment of hairy root cultures of C. roseus and the manipulation of media and other growth conditions for the production of different indole alkaloids have been reported (Parr et al 1988;Toivonen et al 1992;Shanks and Bhadra 1997;Rijhwani and Shanks 1998;Morgan et al 2000). In the present study, we used HPLC to analyse 50 independent hairy root clones belonging to the C1-C3 categories for the production of root-specific indole alkaloids.…”

Section: Hplc Analysis Of Hairy Root Clonesmentioning

confidence: 98%

Growth and terpenoid indole alkaloid production in Catharanthus roseus hairy root clones in relation to left- and right-termini-linked Ri�T-DNA gene integration

et al. 2004

View full text Add to dashboard Cite

Hairy root cultures of Catharanthus roseus var. Prabal were established by infecting the leaves with Agrobacterium rhizogenes agropine-type A4 strain. Two hundred and fifty independent root clones were evaluated for growth, morphology, number of integration of Ri T-DNA genes and alkaloid contents. On the basis of growth pattern, type of branching and number of lateral roots we were able to separate the hairy root clones into four categories. However based on the integration of the Ri T(L)-DNA and T(R)-DNA genes, there were only three different categories of independent hairy root clones-C1 (rolA&B(+)/ags(+)), C2 (rolA&B(-)/ags(+)) and C3 (rolA&B(+)/ags(-)). Southern hybridization analysis revealed both single and multiple copies of T-DNA integration in the root clones. The accumulation of considerable amounts of the root-specific alkaloids ajmalicine and serpentine was observed in the presence of both the T(L)-DNA and T(R)-DNA genes (C1) and the T(L)-DNA gene (C3) alone. Two rolA&B(-) but ags(+) clones (C2) accumulated much less or only very negligible amounts of ajmalicine. The possible role of the T(L)-DNA and T(R)-DNA genes on growth and alkaloid accumulation in these root clones is discussed.

show abstract

The effect of temperature on hairy root cultures of Catharanthus roseus: Growth, indole alkaloid accumulation and membrane lipid composition

Cited by 21 publications

References 13 publications

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

Catharanthus roseus: micropropagation and in vitro techniques

Growth and terpenoid indole alkaloid production in Catharanthus roseus hairy root clones in relation to left- and right-termini-linked Ri�T-DNA gene integration

Contact Info

Product

Resources

About