The effect of temperature on the viability of Demodex folliculorum and Demodex brevis

Zhao, Ya E; Guo, Na; Wu, Li

doi:10.1007/s00436-009-1603-x

Cited by 52 publications

(36 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The temperatures tested in this study were based on Zhao et al (2009), who studied the activity and viability of D. folliculorum and D. brevis at temperatures ranging from -15 to ?60°C. There it was found that both species move weakly below 16°C and survive shorter above 37°C.…”

Section: Discussionmentioning

confidence: 99%

Influence of temperature and medium on viability of Demodex folliculorum and Demodex brevis (Acari: Demodicidae)

Zhao

Guo

2011

Exp Appl Acarol

Self Cite

View full text Add to dashboard Cite

Demodex folliculorum and D. brevis are cosmopolitan, obligatory parasites. Epidemiological studies have established a clear association between these species and various facial diseases in humans. However, not much is known of the ecology of these mites, also because it has proven difficult to culture them. Here we report a laboratory study on the (combined) effects of temperature and culture medium on the viability of both species. Results showed significant differences in the survival time of D. folliculorum and D. brevis among different temperatures and media. Pair-wise comparisons revealed that both species survived longest in serum medium, at a temperature of 16-22 °C. Therefore, we conclude that these conditions allow preservation and maintenance of both Demodex mites.

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of temperature and medium on viability of Demodex folliculorum and Demodex brevis (Acari: Demodicidae)

Zhao

Guo

2011

Exp Appl Acarol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Staphylococcus Epidermidis isolated from patients with rosacea secretes more proteins (of which a lipase) at 37° compared with 30° 55 . The motility of D increases with temperature: at 37°C, the mites are extraordinarily active, but have a shorter survival time 56 . Perhaps an optimal temperature favouring D copulation exists?…”

Section: Demodex and Its Major Pathogenic Mechanismsmentioning

confidence: 99%

Papulopustular rosacea, skin immunity and Demodex: pityriasis folliculorum as a missing link

Forton¹

2011

Acad Dermatol Venereol

142

201

View full text Add to dashboard Cite

Papulopustular rosacea (PPR) is a common facial skin disease, characterized by erythema, telangiectasia, papules and pustules. Its physiopathology is still being discussed, but recently several molecular features of its inflammatory process have been identified: an overproduction of Toll-Like receptors 2, of a serine protease, and of abnormal forms of cathelicidin. The two factors which stimulate the Toll-like receptors to induce cathelicidin expression are skin infection and cutaneous barrier disruption: these two conditions are, at least theoretically, fulfilled by Demodex, which is present in high density in PPR and creates epithelial breaches by eating cells. So, the major pathogenic mechanisms of Demodex and its role in PPR are reviewed here in the context of these recent discoveries. In this review, the inflammatory process of PPR appears to be a consequence of the proliferation of Demodex, and strongly supports the hypothesis that: (1) in the first stage a specific (innate or acquired) immune defect against Demodex allows the proliferation of the mite; (2) in the second stage, probably when some mites penetrate into the dermis, the immune system is suddenly stimulated and gives rise to an exaggerated immune response against the Demodex, resulting in the papules and the pustules of the rosacea. In this context, it would be very interesting to study the immune molecular features of this first stage, named "pityriasis folliculorum", where the Demodex proliferate profusely with no, or a low immune reaction from the host: this entity appears to be a missing link in the understanding of rosacea.

show abstract

“…With the pathogenicity of the mites parasitizing in animals like dog, goat, etc. and human being confirmed successively (Smith 1961;Sheahan and Gaafar 1970;Zhao et al 2010Zhao et al , 2011aZhao et al , b, 2012b, researchers have done some work in the ecology and cultivation of Demodex mites, as well as the therapy of demodicidosis (Heine et al 2005;Xhaxhiu et al 2009;Zhao et al 2009;Fourie et al 2009). It has been reported that the pathogenicity of Demodex is related to population.…”

Section: Discussionmentioning

confidence: 93%

Molecular identification and phylogenetic study of Demodex caprae

Zhao

Juan

et al. 2014

Parasitol Res

View full text Add to dashboard Cite

The DNA barcode has been widely used in species identification and phylogenetic analysis since 2003, but there have been no reports in Demodex. In this study, to obtain an appropriate DNA barcode for Demodex, molecular identification of Demodex caprae based on mitochondrial cox1 was conducted. Firstly, individual adults and eggs of D. caprae were obtained for genomic DNA (gDNA) extraction; Secondly, mitochondrial cox1 fragment was amplified, cloned, and sequenced; Thirdly, cox1 fragments of D. caprae were aligned with those of other Demodex retrieved from GenBank; Finally, the intra- and inter-specific divergences were computed and the phylogenetic trees were reconstructed to analyze phylogenetic relationship in Demodex. Results obtained from seven 429-bp fragments of D. caprae showed that sequence identities were above 99.1% among three adults and four eggs. The intraspecific divergences in D. caprae, Demodex folliculorum, Demodex brevis, and Demodex canis were 0.0-0.9, 0.5-0.9, 0.0-0.2, and 0.0-0.5%, respectively, while the interspecific divergences between D. caprae and D. folliculorum, D. canis, and D. brevis were 20.3-20.9, 21.8-23.0, and 25.0-25.3, respectively. The interspecific divergences were 10 times higher than intraspecific ones, indicating considerable barcoding gap. Furthermore, the phylogenetic trees showed that four Demodex species gathered separately, representing independent species; and Demodex folliculorum gathered with canine Demodex, D. caprae, and D. brevis in sequence. In conclusion, the selected 429-bp mitochondrial cox1 gene is an appropriate DNA barcode for molecular classification, identification, and phylogenetic analysis of Demodex. D. caprae is an independent species and D. folliculorum is closer to D. canis than to D. caprae or D. brevis.

show abstract

The effect of temperature on the viability of Demodex folliculorum and Demodex brevis

Cited by 52 publications

References 4 publications

Influence of temperature and medium on viability of Demodex folliculorum and Demodex brevis (Acari: Demodicidae)

Influence of temperature and medium on viability of Demodex folliculorum and Demodex brevis (Acari: Demodicidae)

Papulopustular rosacea, skin immunity and Demodex: pityriasis folliculorum as a missing link

Molecular identification and phylogenetic study of Demodex caprae

Contact Info

Product

Resources

About