The Effect of Temperature on the Conversion of Protochlorophyll to Chlorophyll a in Etiolated Barley Leaves.

Smith, James H. C.; Benitez, Allen

doi:10.1104/pp.29.2.135

Cited by 89 publications

(23 citation statements)

References 10 publications

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“…However, the reaction is not strictly photochemical, since it is greatly inhibited at low temperatures, e.g. only 40% phototransformation occurs in barley at -60°C (Smith & Beneitz, 1954), suggesting that the process must also involve some restricted molecular collisions (Boardman, 1966). It is probable that the photoconversion involves an initial, temperature-independent, photoexcitation of the pigment complex, followed by a hydrogen-transfer step showing a much higher temperature coefficient.…”

Section: Resultsmentioning

confidence: 99%

Reconstitution of chlorophyllide formation by isolated etioplast membranes

Griffiths¹

1978

Biochemical Journal

330

272

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1. The reconstitution of chlorophyllide biosynthesis by barley etioplast membranes is described. 2. The process is dependent on the addition of NADPH and protochlorophyllide and on illumination, which can be either continuous or intermittent. 3. The reconstituted process involves spectroscopically similar intermediates to the native reaction in whole leaves. 4. Steps in the process are an initial enzymic formation in the dark of a photoactive complex, P638/652 (probably a ternary protochlorophyllide-NADPH-enzyme complex), followed by a very rapid light-dependent hydrogen transfer from the NADPH to the protochlorophyllide giving chlorophyllide, finally releasing the enzyme for repeating the process. 5. A continuous assay for the system regenerating complex P638/652 was devised on the basis of monitoring chlorophyllide formation.6. The pH optimum of the reaction is at 6.9 and Km values for protochlorophyllide and NADPH are 0.46 and 35,uM respectively. 7. The reaction is associated specifically with the etioplast membrane fraction. 8. Activities of the system assayed in vitro are more than adequate to account for rates of chlorophyll formation in vivo.

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Section: Resultsmentioning

confidence: 99%

Reconstitution of chlorophyllide formation by isolated etioplast membranes

Griffiths¹

1978

Biochemical Journal

330

272

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“…This, by virtue of PORB, was reduced to zinc pheophorbide a (5), the zinc analog of Chlide a (6). The existence of analogous higher molecular weight light harvesting structures in vivo was inferred from previously reported energy transfer reactions, taking place from so-called photoinactive Pchlide to photoactive Pchlide and from photoinactive Pchlide to Chlide, in prolamellar bodies before and after flash light illumination (7)(8)(9)(10)(11)(12).…”

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confidence: 98%

In Vitro Reconstitution of Light-harvesting POR-Protochlorophyllide Complex with Protochlorophyllides a and b

Reinbothe¹,

Buhr²,

Pollmann³

et al. 2003

Journal of Biological Chemistry

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“…11). Both before and after flash light illumination, energy transfer was observed, taking place from photoinactive Pchlide to photoactive Pchlide in etiolated plants and from photoinactive Pchlide to Chlide in preflashed plants (12)(13)(14)(15)(16)(17).…”

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confidence: 99%