The effect of temperature on the embryonic development of barramundi, the Australian strain of Lates calcarifer (Bloch) using current hatchery practices

Thépot, Valentin; Jerry, Dean R.

doi:10.1016/j.aqrep.2015.09.002

Cited by 21 publications

(11 citation statements)

References 21 publications

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“…The incubation trial showed that higher temperatures accelerated the progress of embryo development (as determined by the time they entered in blastula, pharyngula, segmentation and hatch stages). This association between environmental temperature and hatching time has previously been reported in other fish species including sole (Campos et al, 2013c;Camus and Koutsikopoulos, 1984;Martell et al, 2005;2006;Radonic et al, 2005;Thépot and Jerry, 2015). The delay of ~10-15h between the hatching times reported by Campos et al (2013c) and in our study using a similar thermal range may be due to the developmental stage at which the experiments commenced (3 h in blastula Campos et al (2013c) and 8h in mid-gastrula in our study).…”

Section: Discussionsupporting

confidence: 89%

“…This notable response to environmental temperature is linked to the absence of thermal homeostasis (they are poikilotherms) and the specific evolutionary consequences for development are relatively poorly explored. Normally, early life stages develop faster at higher temperatures due to the modifications induced in molecular and metabolic responses (Campos et al, 2013c;Camus and Koutsikopoulos, 1984;Das et al, 2006;Martell et al, 2006;Politis et al, 2017b;Radonic et al, 2005;Thépot and Jerry, 2015). However, when the environmental temperature is outside of the thermal tolerance range, survival rates decrease and the incidence of malformations substantially increases (Das et al, 2006;Little et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Short- and long-term effects on growth and expression patterns in response to incubation temperatures in Senegalese sole

et al. 2018

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In this study, the short-and long-term effects of embryo incubation temperatures (16, 18 and 20ºC) on development and growth of the flatfish Senegalese sole (Solea senegalensis) was determined by investigating the expression patterns of the epigenetic regulators DNA methyltransferases (dnmt) and histone 3 (H3) and genes belonging to the retinoic acid (RA), insulin-like growth factors (IGFs) and hypothalamic-pituitary-thyroid (HPT) axes. Results indicated that egg incubation temperature affected embryo development, but not survival, and incubation at 16ºC significantly delayed development. Coincident with these effects, levels of muscle-specific dnmt3aa transcripts and histone H3 protein levels were significantly different between the 16 and 20ºC groups at hatch. The larvae from eggs incubated at 20ºC relative to the 16ºC group had significantly higher transcript levels of four genes belonging to the HPT axis (trhr1a, tshr, thrb and dio2), four genes of the RA axis (aldh1a2, cyp26a1, rara2, rarg), igfbp1 and the glycolytic enzyme gapdh2. Taken together the data suggest that higher egg incubation temperatures enhance energy production, which accelerates cell proliferation and larval development and that hatching is a key moment for the regulation of epigenetic mechanisms.Long-term effects of egg and larval incubation temperatures were revealed by a higher mRNA abundance of the thyroid-related genes tgb and tpo and the RA degrading enzyme cyp26a1 in preand metamorphic larvae when they were incubated at 20ºC as embryos and may be related to the earlier initiation of metamorphosis in the pelagic larval stages. Evaluation of growth in pelagic larvae and juveniles after weaning (one trial from 42 to 119 and another from 164 to 247 days post-hatch using a longitudinal approach) revealed that juveniles from embryos incubated at 20ºC had a higher growth rate. All these data demonstrate that the thermal regime during embryogenesis modulated mechanisms that regulate larval plasticity and caused imprinting evident in juvenile sole as persistent changes in key endocrinological pathways and growth performance.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Short- and long-term effects on growth and expression patterns in response to incubation temperatures in Senegalese sole

et al. 2018

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“…In this study, fertilized eggs were incubated at two temperatures within the physiological range at which spontaneous spawning occurs in the wild and in captivity [65]. An inverse relationship between the incubation temperature and development was observed as has previously been reported in several fish species including sole [38, 66–69]. A previous study in S. senegalensis using different incubation temperatures until hatch showed that temperature modified growth and muscle cellularity of benthic post-larvae [38].…”

Section: Discussionmentioning

confidence: 94%

Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis

et al. 2017

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BackgroundThe identification of DNA methyltransferases (Dnmt) expression patterns during development and their regulation is important to understand the epigenetic mechanisms that modulate larval plasticity in marine fish. In this study, dnmt1 and dnmt3 paralogs were identified in the flatfish Solea senegalensis and expression patterns in early developmental stages and juveniles were determined. Additionally, the regulation of Dnmt transcription by a specific inhibitor (5-aza-2′-deoxycytidine) and temperature was evaluated.ResultsFive paralog genes of dnmt3, namely dnmt3aa, dnmt3ab, dnmt3ba, dnmt3bb.1 and dnmt3bb.2 and one gene for dnmt1 were identified. Phylogenetic analysis revealed that the dnmt gene family was highly conserved in teleosts and three fish-specific genes, dnmt3aa, dnmt3ba and dnmt3bb.2 have evolved. The spatio-temporal expression patterns of four dnmts (dnmt1, dnmt3aa, dnmt3ab and dnmt3bb.1) were different in early larval stages although all of them reduced expression with the age and were detected in neural organs and dnmt3aa appeared specific to somites. In juveniles, the four dnmt genes were expressed in brain and hematopoietic tissues such as kidney, spleen and gills. Treatment of sole embryos with 5-aza-2′-deoxycytidine down-regulated dntm1 and up-regulated dntm3aa. Moreover, in lecithotrophic larval stages, dnmt3aa and dnmt3ab were temperature sensitive and their expression was higher in larvae incubated at 16 °C relative to 20 °C.ConclusionFive dnmt3 and one dnmt1 paralog were identified in sole and their distinct developmental and tissue-specific expression patterns indicate that they may have different roles during development. The inhibitor 5-aza-2′-deoxycytidine modified the transcript abundance of dntm1 and dntm3aa in embryos, which suggests that a regulatory feedback mechanism exists for these genes. The impact of thermal regime on expression levels of dnmt3aa and dnmt3ab in lecithotrophic larval stages suggests that these paralogs might be involved in thermal programing.Electronic supplementary materialThe online version of this article (doi:10.1186/s12861-017-0154-0) contains supplementary material, which is available to authorized users.

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“…Barramundi ( L. calcarifer ) larvae were supplied by Jarrod Guppy and Adrien Marc (Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University). After injection with luteinizing hormone-releasing hormone analogue, 12 broodstock barramundi (4 females and 8 males) underwent communal mass spawning as described in Thépot and Jerry (2015) . After hatching (14 hpf; average hatch tank conditions: 30°C, 8.1 pH, 30 ppt salinity, >5.0 mg L −1 O 2 ), larvae were transferred to the SeaSim.…”

Section: Methodsmentioning

confidence: 99%

Automated flow control of a multi-lane swimming chamber for small fishes indicates species-specific sensitivity to experimental protocols

Illing

Severati

Hochen

et al. 2021

Conservation Physiology

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In fishes, swimming performance is considered an important metric to measure fitness, dispersal and migratory abilities. The swimming performance of individual larval fishes is often integrated into models to make inferences on how environmental parameters affect population-level dynamics (e.g. connectivity). However, little information exists regarding how experimental protocols affect the swimming performance of marine fish larvae. In addition, the technical setups used to measure larval fish swimming performance often lack automation and accurate control of water quality parameters and flow velocity. In this study, we automated the control of multi-lane swimming chambers for small fishes by developing an open-source algorithm. This automation allowed us to execute repeatable flow scenarios and reduce operator interference and inaccuracies in flow velocity typically associated with manual control. Furthermore, we made structural modifications to a prior design to reduce the areas of lower flow velocity. We then validated the flow dynamics of the new chambers using computational fluid dynamics and particle-tracking software. The algorithm provided an accurate alignment between the set and measured flow velocities and we used it to test whether faster critical swimming speed (Ucrit) protocols (i.e. shorter time intervals and higher velocity increments) would increase Ucrit of early life stages of two tropical fish species [4–10-mm standard length (SL)]. The Ucrit of barramundi (Lates calcarifer) and cinnamon anemonefish (Amphiprion melanopus) increased linearly with fish length, but in cinnamon anemonefish, Ucrit started to decrease upon metamorphosis. Swimming protocols using longer time intervals (more than 2.5 times increase) negatively affected Ucrit in cinnamon anemonefish but not in barramundi. These species-specific differences in swimming performance highlight the importance of testing suitable Ucrit protocols prior to experimentation. The automated control of flow velocity will create more accurate and repeatable data on swimming performance of larval fishes. Integrating refined measurements into individual-based models will support future research on the effects of environmental change.

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The effect of temperature on the embryonic development of barramundi, the Australian strain of Lates calcarifer (Bloch) using current hatchery practices

Cited by 21 publications

References 21 publications

Short- and long-term effects on growth and expression patterns in response to incubation temperatures in Senegalese sole

Short- and long-term effects on growth and expression patterns in response to incubation temperatures in Senegalese sole

Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis

Automated flow control of a multi-lane swimming chamber for small fishes indicates species-specific sensitivity to experimental protocols

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