The effect of temperature and humidity on the growth of Trichophyton mentagrophytes spores on human stratum corneum in vitro

Knight, Arthur

doi:10.1111/j.1365-2230.1976.tb01411.x

Cited by 7 publications

(2 citation statements)

References 11 publications

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“…However, it is difficult to compare results from these manuscripts with the results of the current study because numerous changes in dermatophyte taxonomy make it difficult to determine if the same species are being considered 18 . One study defined the optimal growth temperature for T. mentagrophytes as 27–33°C, 19 which is considerably higher than the temperature range of 20.6–25.6°C observed in the current work. Incubation of plates in the XLIGHT condition brought the cultures closer to the optimal target range, consistent with the observed XLIGHT growth‐stimulatory effect.…”

Section: Discussionmentioning

confidence: 75%

Effect of light exposure on growth rate of veterinary clinical dermatophyte isolates

Elwart

Pieper

et al. 2021

Veterinary Dermatology

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Background -Veterinary textbooks and literature suggest that exposure to light is inhibitory to growth of clinical dermatophyte isolates.Hypothesis/objectives -We hypothesized that this idea was derived from experiments that examined the effect of high doses of ultraviolet and visible light exposure on dermatophyte growth, and that exposure to typical room lighting would not adversely affect dermatophyte growth rate.Methods and materials -Isolates of common veterinary dermatophytes (three each of Microsporum canis, Nannizia gypsea and Trichophyton benhamiae) were exposed to typical fluorescent room lighting, incubated in a closed drawer, or exposed at close range to fluorescent wide-spectrum light. Dermatophytes were grown on Sabouraud Dextrose Agar (SAB) and Dermatophyte Test Medium (DTM). Colony diameter was measured and growth rate (expressed as colony diameter increase mm/day) calculated at the linear portion of the culture growth curve. Statistical analyses compared growth rates across the various incubation conditions and among dermatophyte isolates.Results -There was little difference in growth rate between cultures incubated under typical fluorescent room lighting and those placed in the dark. Exposure to the close-range light increased growth rate as a consequence of the elevated incubation temperature created by the lamp. Significant differences in growth rate were noted among strains of the same dermatophyte species. Dermatophytes grew more rapidly on SAB than DTM agar.Conclusions and clinical relevance -Exposure to typical lighting conditions in a clinical environment does not inhibit growth of dermatophyte colonies. Veterinary clinicians may conduct routine dermatophyte cultures without incubating them in the dark.

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Section: Discussionmentioning

confidence: 75%

Effect of light exposure on growth rate of veterinary clinical dermatophyte isolates

Elwart

Pieper

et al. 2021

Veterinary Dermatology

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“…Storing conidia at 100% humidity killed the samples after only 9 days at 22 o C 8 . Other works indicated that Trichophyton mentagrophytes, the other main dermatophytes related to onychomycosis, has a very narrow humidity range of 95-98% and that different levels of humidity are better or worse for different stages of T. mentagrophytes-high humidity is necessary for arthrospore formation but reduced humidity necessary for maturation 19 . While T. mentagrophytes and T. rubrum are not the same, they are similar enough to warrant further studies investigating the effect of humidity and spore creation and growth 6,19,20 .…”

Section: Vacuummentioning

confidence: 99%

Effect of vacuum and thermal shock on laser treatment of Trichophyton rubrum (toenail fungus)

Aguilar

Sun

Carlier³

et al. 2010

Photonic Therapeutics and Diagnostics VI

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The eradication of Trichophyton rubrum has been attempted via laser irradiation because it could result advantageous relative to current clinical therapies. Anticipating that the necessary thermal effects could unintentionally damage the underlying toe dermal layer, we have explored two auxiliary approaches: (a) laser irradiation under vacuum pressure, with and without water dousing and, (b) cooling followed by laser heating (thermal shock). The rationale is that at low pressures, the temperature necessary to achieve water evaporation/boiling is significantly reduced, thus requiring lower fluences. Similarly, a thermal shock induced by cooling followed by laser irradiation may require lower fluences to achieve fungus necrosis. For all experiments presented we use a Cooltouch, model CT3 plus, 1320 nm laser to irradiate fungi colonies. The vacuum pressure experiments exposed fungi colonies to a subatmospheric pressure of 84.7 kPa (25 inHg) with and without water dousing for 5 min, followed by irradiation with 4.0 J/cm 2 fluence and 40-90 J total energies. The thermal shock experiments consisted of three sections at 4.8 J/cm 2 : cooling the fungus to 0 o C at 0.39 o C/min and then irradiating to 45-60 o C; cooling to -20 o C at 1.075 o C/min and irradiating to 45 o C; and cooling to -20 o C at 21.5 o C/min and irradiating to 45 o C. Fungus growth rate over a 1-week period assessed the feasibility of these procedures. Results indicated both approaches hamper the growth rate of fungi colonies relative to untreated control samples, especially water dousing under vacuum conditions and slow cooling rate preceding irradiation for thermal shock effect.

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Microbial Ecology of the Human Skin

Noble¹,

Pitcher²

1978

Advances in Microbial Ecology

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The effect of temperature and humidity on the growth of Trichophyton mentagrophytes spores on human stratum corneum in vitro

Cited by 7 publications

References 11 publications

Effect of light exposure on growth rate of veterinary clinical dermatophyte isolates

Effect of light exposure on growth rate of veterinary clinical dermatophyte isolates

Effect of vacuum and thermal shock on laser treatment of Trichophyton rubrum (toenail fungus)

Microbial Ecology of the Human Skin

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