The Effect of Taxifolin on Cisplatin-Induced Pulmonary Damage in Rats: A Biochemical and Histopathological Evaluation

Abstract

The effect of taxifolin on cisplatin-induced oxidative pulmonary damage was investigated biochemically and histopathologically in male albino Wistar rats. There were four groups, with six animals in each group: 50 mg/kg of taxifolin plus 2.5 mg/kg of cisplatin (TC) group, 2.5 mg/kg of cisplatin only (CIS) group, 50 mg/kg of taxifolin only (TG) group, and a healthy control group (HG). In terms of the experimental procedure, the animals in the TC and TG groups were first treated via oral gavage. The CIS and HG groups received distilled water as solvent, respectively. One hour later, the TC and CIS groups received cisplatin at a dose of 2.5 mg/kg (injected intraperitoneally). Taxifolin, cisplatin, and the distilled water were administered at the indicated dose and volume, using the same method daily for 14 d. At the end of this period, the animals were killed with a high dosage of thiopental anaesthesia (50 mg/kg). Blood and lung tissue samples were taken for biochemical (malondialdehyde (MDA), myeloperoxidase (MPO), total glutathione (tGSH), and 8-hydroxy-2 deoxyguanosine (8-OHdG)) analyses and histopathological examinations. The biochemical and histopathological results in the TC and HG groups were then compared with those in the CIS group. Cisplatin increased the levels of MDA, myeloperoxidase, and 8-OHdG, a marker of oxidative DNA damage, and reduced the amount of tGSH in the lung tissue. Moreover, severe alveolar damage, including oedema and extensive alveolar septal fibrosis, in addition to infiltration of polymorphic nuclear leucocytes and haemorrhagic foci, was observed in the CIS group. These histopathological findings demonstrate that taxifolin provides protection against pulmonary oxidative stress by preventing increases in oxidant parameters and decreases in antioxidants.

Lycopene and Caffeic Acid Phenethyl Ester Affect Caspase-3 Activity, but Do Not Alter the NO Pathway in Lung Tissue Damage Induced by Cisplatin

“…Application of CP is related to a significant increase in the ROS production in various tissues, including lungs [15]. The formed ROS further induce oxidative modification of cell-building molecules – lipids and proteins, causing the formation of unfunctional molecules which, at the end, results in the cell death.…”

“…The source of hypochlorous acid (a form of ROS) is a polymorphonuclear cell related enzyme MPO [15]. Thus, by inhibiting CP-induced increase in the MPO activity (Table 1), through the inhibition of the inflammatory response (Fig.…”

Possible Protective Effect of Silver Nanoparticles against Cisplatin Induced Pulmonary Inflammation in Rat Model

“…Group 3 received AgNps (10ppm) [11]. Group 4 induced group received single interperitoneal dose of CP (2.5mg/kg ) [12]. Group 5 and 6 1h after CP administration animals received AgNPs (5ppm and 10ppm).…”

Taksi̇foli̇ni̇n Siçanlarda Akri̇lami̇dle İndüklenen Mi̇de Hasarina Karşi Koruyucu Etki̇si̇ni̇n Hi̇stopatoloji̇k Değerlendi̇ri̇lmesi̇

“…AKR'in mide toksisitesine karşı koruyucu etkisini araştırdığımız Taksifolin, turunçgiller ve soğanda yüksek oranda bulunan bir flavonoiddir (8).Litaretürde flavonoidlerin, ROS meydana gelmesinde görevli enzimatik reaksiyonları durdurduğu rapor edilmiştir (9).Taksifolin'in çeşitli organ dokularında oksidan miktarının azaltıp antioksidan miktarını artırarak, dokuları oksidatif hasardan koruduğu bildirilmiştir (10)(11)(12).Flavonoid ve benzeri antioksidan içeren gıdaların çeşitli hastalıkların önlenmesi ve tedavisi amacı ile alınması, tüm dünyada sürekli yaygınlaşmaktadır. Gıdalar ile yüksek oranlarda alınan ve insan sağlığı açısından faydalı olan birçok ikincil metabolitlerin sınıfını temsil eden flavonoidler artan şekilde araştırma konusu olmuştur.…”

“…Litaretürde flavonoidlerin, reaktif oksijen türlerinin (ROS) meydana gelmesinden sorumlu enzimatik süreçleri durdurduğu bildirilmiştir (9). Taksifolin'in bazı organ dokularında oksidan artışını ve antioksidan azalmasını durdurarak dokuları oksidatif olumsuz etkilerden koruduğu rapor edilmiştir (10)(11)(12). Bu çalışmada; AKR ile oluşturulan mide hasarında, Taksifolin'in koruyucu etkisinin olup olmadığını, sıçanlarla oluşturulan deneysel bir hayvan modeli ile histopatolojik açıdan ortaya koymayı amaçladık.…”