The effect of sympathetic nerve stimulation on serum gastrin, gastric acid secretion and mucosal blood flow responses to meat extract stimulation in anaesthetized cats.

Abstract

SUMMARY1. Chloralose anaesthetized cats were prepared with fundic and antral pouches. Fundic mucosal blood flow was measured by the amidopyrine technique and serum gastrin was measured by radioimmunoassay.2. Meat extract suspension in the pyloric antrum produced a highly significant sixfold increase in arterial serum gastrin concentration (P < 0-001).3. The mean ratio of the fundic mucdsal blood flow to acid secretary responses (AMBF/LAH+ ratio) of 0-142 + 0-026 (25) ml./,uequiv H+, is very similar to the values previously published for exogenous gastrin stimulation. 4. Splanchnic nerve stimulation, during responses to meat extract stimulation, produced significant reductions in gastric acid secretion (P < 0-025), fundic mucosal blood flow (P < 0-02), arterial serum gastrin concentration (P < 0-01) and gastrin delivered to the mucosa (P < 0.001).5. In the 30 min period following the end of splanchnic nerve stimulation only arterial serum gastrin concentration remained significantly reduced.

Effect of Protein Intake on Gastrin Secretion in Ruminants

“…The lack of difference in gastrin responses between diets in the sheep experiment suggests either that, once modified by rumen fermentation, the protein content of the diets was not sufficiently different to produce dissimilar gastrin responses or that there is less of a reliance on protein and its products of digestion for stimulation of gastrin release than there is in non-ruminant species. In comparison, for example, post-feeding arterial gastrin concentrations increase up to sixfold on feeding a meat extract to cats (29 5-200 9 pg gastrin 17/ml) (Blair et al 1975b).…”

“…Measurement of plasma gastrin concentrations was achieved using a radioimmunoassay based on that reported by Blair, Grund, Reed, Sanders, Sanger & Shaw (1975b). The assay utilized synthetic human gastrin 17 (non-sulphated) as standard and as the basis for antiserum and tracer preparations.…”

Inhibition of vagally mediated gastric acid secretion by activation of central prostanoid EP3 receptors in urethane‐anaesthetized rats

“…Two ml of gastric solution prewarmed to 38°C was instilled and replaced at intervals of 15 min. The solution consisted of a 1/5 (v/v) mixture of glycine and mannitol adjusted to 300 mOsmol and pH 3.5 by the addition of 0.1 N HC1, according to Blair et al (1975 (sulprostone,misoprostol, or their vehicles in all experiments, a stainless steel cannula (0.35 mm outer diameter) was inserted into the lateral bregma, L 1.5 mm from the midline, H 4.0 mm below the surface of the brain according to the rat brain atlas of Paxinos & Watson (1986). Vehicle or prostanoid EP receptor agonist dissolved in saline was slowly injected into the right lateral ventricle in a volume of 10 pl with a 50 1l Hamilton syringe 60 min after the start of the vagal stimulation.…”

Effect of d-Nicotine on the l-Nicotine-lnduced Increase in Gastric Acid Secretion in Rats

“…The composition of the artificial gastric juice was a 1 :5 (v/v) mixture of glycine and mannitol adjusted to 300 mosM and pH 3.5 by addition of 0.1 N HCI, according to the method of Blair et al (8). Acid output was determined by titration of gastric samples to pH 7.0 with 0.01 N NaOH.…”