The effect of surgical denervation on prevention of excessive dermal scarring: A study on rabbit ear hypertrophic scar model

Yağmur, Çağlayan; Güneren, Ethem; Kefeli, Mehmet; Ogawa, Rei

doi:10.1016/j.bjps.2011.04.028

Cited by 15 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Capsaicin‐treated rats had 30%–40% reduced wound healing and increased presence of α‐smooth muscle actin (α‐SMA) . This was also seen in a rabbit wound model where wounds with surgically excised nerves exhibited poor wound healing and decreased macrophage presence than controls . It is possible that capsaicin's analgesic effects are inhibiting cellular activity resulting in poor wound healing but it is uncertain what cell type capsaicin is affecting.…”

Section: Resultsmentioning

confidence: 97%

Capsaicin reduces PLGA‐induced fibrosis by promoting M2 macrophages and suppressing overall inflammatory Response

Truong

Jones

2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Capsaicin reduced poly(lactic-co-glycolic) acid (PLGA)-induced fibrosis by promoting IL-10 secretion and suppressing alpha-smooth muscle actin (α-SMA) expression. The lifetime and efficacy of tissue engineering scaffolds are determined by the foreign body response. In this study, we investigated the in vitro and in vivo effects of capsaicin to reduce biomaterial-induced fibrosis. RAW 264.7 cells cultured on PLGA films with capsaicin responded with significant (p < 0.05) upregulation in M2 markers arginase-1 and IL-10 and downregulation of M1 markers iNOS and IL-12, demonstrating the potential of capsaicin to reduce PLGA-induced inflammation. Subsequent animal studies were conducted where PLGA and capsaicin-embedded PLGA discs were implanted in C57BL/6 mice for 2 and 14 days. Explanted capsaicin-embedded PLGA implants had 40% less collagen than PLGA-only implants. Capsaicin caused a 35% increase in IL-10 which played a key role in suppressing fibrosis. Macrophage phenotype markers in peritoneal cells and adherent cells were unaffected by capsaicin; however, capsaicin suppressed the myofibroblast marker α-SMA in adherent cells by day 14. Overall, our results revealed that capsaicin reduced biomaterial-induced fibrosis and demonstrates that capsaicin has the potential to extend the lifetime of a tissue engineering scaffold when used in long-term drug release applications from hydrophobic biomaterials. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2424-2432, 2018.

show abstract

Section: Resultsmentioning

confidence: 97%

Capsaicin reduces PLGA‐induced fibrosis by promoting M2 macrophages and suppressing overall inflammatory Response

Truong

Jones

2018

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Mechanosensitive nociceptors release neuropeptides through nerve endings, which cause vasodilatation, neurogenic inflammation, and fibroblast proliferations in wounds. 21 Yagmur et al 22 reported that hypertrophic scars were prevented in animal models with sensory denervation. Also, mouse models with biomechanical loading devices reproduced hypertrophic scar formation, which was mediated by Akt antiapoptotic pathway.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Risk factors for hypertrophic surgical scar development after thyroidectomy

Kim

Sung

Kim

et al. 2012

Wound Repair Regeneration

View full text Add to dashboard Cite

Postoperative neck scarring is a major concern for patients who undergo thyroid surgery; however, the treatments for hypertrophic scars are generally considered by patients to provide unsatisfactory outcomes. Therefore, risk factors should be identified and prevention of these factors is considered to be critical in management. We reviewed the medical records of 96 thyroidectomy patients who were divided into two groups based on scar type: patients with hypertrophic (n = 61) and linear flat scars (n = 35). Multivariable logistic regression model was developed to identify risk factors for developing hypertrophic scar. There was no significant difference between the two groups in terms of age, gender ratio, tumor type, and type of operation. Multivariable analysis showed that hypertrophic scar development was associated with scars located within 1 cm above the sternal notch (odds ratio [OR] = 5.94, p = 0.01), prominent sternocleidomastoid muscles (OR = 12.03, p < 0.01), and a high body mass index (OR = 1.33, p = 0.01). The area under the receiver operating characteristic curve for risk factors was 0.85. Development of hypertrophic scar after thyroidectomy was found to be associated with specific preoperative factors such as incision site near the sternal notch, prominent sternocleidomastoid muscles, and high body mass index.

show abstract

“…Although the rabbit vascular ischemia model is commonly used, there are few animal models of diabetic peripheral neuropathy, most of which are rodents 21-23 . The neuropathy/denervation model that we have used, although not perfect, has been used to study the effect of sensory denervation on hypertrophic scarring in absence of diabetes and vascular ischemia 24 . Due to the bidirectional connection between nerves and inflammatory cells, in a model of ‘denervation only’, we could expect partial wound-healing impairment most likely through dysregulation of inflammation and deficiency of neuropeptides.…”

Section: Discussionmentioning

confidence: 99%

Expression of neuropeptides and cytokines in a rabbit model of diabetic neuroischemic wound healing

Nabzdyk

Kuchibhotla²,

Guthrie

et al. 2013

Journal of Vascular Surgery

View full text Add to dashboard Cite

Objective The present study is designed to understand the contribution of peripheral vascular disease and peripheral neuropathy to the wound-healing impairment associated with diabetes. Using a rabbit model of diabetic neuroischemic wound-healing we investigated rate of healing, leukocyte infiltration and expression of cytokines, Interleukin (IL)-8 and IL-6, and, neuropeptides, Substance P (SP) and Neuropeptide Y (NPY). Design of study Diabetes was induced in White New Zealand rabbits by administering alloxan while control rabbits received saline. Ten days later animals in both groups underwent surgery. One ear served as a sham and the other was made ischemic (ligation of central+rostral arteries), or neuroischemic (ischemia+ resection of central+rostral nerves). Four, 6mm punch biopsy wounds were created in both ears and wound-healing was followed for ten days using computerized planimetry. Results Non-diabetic sham and ischemic wounds healed significantly more rapidly than diabetic sham and ischemic wounds. Healing was slowest in neuroischemic wounds, irrespective of diabetic status. A high M1/M2 macrophage ratio and a high pro-inflammatory cytokine expression, both indicators of chronic-proinflammatory state, and low neuropeptide expression were seen in pre-injury diabetic skin. Post-injury, in diabetic wounds M1/M2 ratio remained high, the reactive increase in cytokine expression was low and neuropeptide expression was further decreased in neuroischemic wounds. Conclusion This rabbit model illustrates how a combination of a high M1/M2 ratio, a failure to mount post-injury cytokine response as well as a diminished neuropeptide expression contribute to wound-healing impairment in diabetes. The addition of neuropathy to ischemia leads to equivalently severe impaired wound-healing irrespective of diabetes status, suggesting that in the presence of ischemia, loss of neuropeptide function contributes to the impaired healing associated with diabetes.

show abstract

The effect of surgical denervation on prevention of excessive dermal scarring: A study on rabbit ear hypertrophic scar model

Cited by 15 publications

References 36 publications

Capsaicin reduces PLGA‐induced fibrosis by promoting M2 macrophages and suppressing overall inflammatory Response

Capsaicin reduces PLGA‐induced fibrosis by promoting M2 macrophages and suppressing overall inflammatory Response

Risk factors for hypertrophic surgical scar development after thyroidectomy

Expression of neuropeptides and cytokines in a rabbit model of diabetic neuroischemic wound healing

Contact Info

Product

Resources

About