The effect of surface wettability on induction and growth of neurites from the PC-12 cell on a polymer surface

Lee, Sang Jin; Khang, Gilson; Lee, Young Moo; Lee, Hai Bang

doi:10.1016/s0021-9797(02)00163-7

Cited by 141 publications

(116 citation statements)

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“…Both discrete specimens and surface energy gradients were used in these studies and many different outcomes have been observed. In some studies, cell functions are enhanced on hydrophilic surfaces, 119,[125][126][127] whereas in others, cell functions are enhanced on hydrophobic surfaces. [128][129][130][131] While in other cases, surface energy has no effect on cell functions 131,132 or cell functions have a maximum at an intermediate surface energy.…”

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confidence: 99%

Biomimetic micro/nanostructured functional surfaces for microfluidic and tissue engineering applications

2011

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1 This paper reviews our work on the application of ultrafast pulsed laser micro/ nanoprocessing for the three-dimensional ͑3D͒ biomimetic modification of materials surfaces. It is shown that the artificial surfaces obtained by femtosecond-laser processing of Si in reactive gas atmosphere exhibit roughness at both micro-and nanoscales that mimics the hierarchical morphology of natural surfaces. Along with the spatial control of the topology, defining surface chemistry provides materials exhibiting notable wetting characteristics which are potentially useful for open microfluidic applications. Depending on the functional coating deposited on the laser patterned 3D structures, we can achieve artificial surfaces that are ͑a͒ of extremely low surface energy, thus water-repellent and self-cleaned, and ͑b͒ responsive, i.e., showing the ability to change their surface energy in response to different external stimuli such as light, electric field, and pH. Moreover, the behavior of different kinds of cells cultured on laser engineered substrates of various wettabilities was investigated. Experiments showed that it is possible to preferentially tune cell adhesion and growth through choosing proper combinations of surface topography and chemistry. It is concluded that the laser textured 3D micro/ nano-Si surfaces with controllability of roughness ratio and surface chemistry can advantageously serve as a novel means to elucidate the 3D cell-scaffold interactions for tissue engineering applications.

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confidence: 99%

Biomimetic micro/nanostructured functional surfaces for microfluidic and tissue engineering applications

2011

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“…It is noteworthy that the physicochemical characteristics of the membranes in terms of hydrophobicity/hydrophilicity (wettability) could guide neuronal cell responses by strongly influencing cell adhesion and growth. It has been reported that neuronal cells preferentially adhere to surfaces with moderate hydrophilicity [Lee et al, 2003]. Indeed, in the case of superhydrophilic surfaces, the proteins that mediate cell adhesion are not well bound on the surface and do not ensure a strong and valuable cell attachment.…”

Section: Discussionmentioning

confidence: 99%

Neuronal Differentiation Modulated by Polymeric Membrane Properties

Morelli

Piscioneri²,

Drioli³

et al. 2017

Cells Tissues Organs

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In this study, different collagen-blend membranes were successfully constructed by blending collagen with chitosan (CHT) or poly(lactic-co-glycolic acid) (PLGA) to enhance their properties and thus create new biofunctional materials with great potential use for neuronal tissue engineering and regeneration. Collagen blending strongly affected membrane properties in the following ways: (i) it improved the surface hydrophilicity of both pure CHT and PLGA membranes, (ii) it reduced the stiffness of CHT membranes, but (iii) it did not modify the good mechanical properties of PLGA membranes. Then, we investigated the effect of the different collagen concentrations on the neuronal behavior of the membranes developed. Morphological observations, immunocytochemistry, and morphometric measures demonstrated that the membranes developed, especially CHT/Col30, PLGA, and PLGA/Col1, provided suitable microenvironments for neuronal growth owing to their enhanced properties. The most consistent neuronal differentiation was obtained in neurons cultured on PLGA-based membranes, where a well-developed neuronal network was achieved due to their improved mechanical properties. Our findings suggest that tensile strength and elongation at break are key material parameters that have potential influence on both axonal elongation and neuronal structure and organization, which are of fundamental importance for the maintenance of efficient neuronal growth. Hence, our study has provided new insights regarding the effects of membrane mechanical properties on neuronal behavior, and thus it may help to design and improve novel instructive biomaterials for neuronal tissue engineering.

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“…PC-12 cells (KCLB 21721, Korea Cell Line Bank, Korea) were cultured in Dulbecco's modified Eagles medium (DMEM, GIBCO BRL Co., USA) with L-glutamine, supplemented with 15% in activated donor calf serum (CS, GIBCO BRL) and antibiotic/antimycotic (penicillin 10,000 U/mL, streptomycin 10,000 cgm/mL, and amphotericin B 25 μg/mL, GIBCO BRL) in tissue culture polystyrene flask (Falcon, Co., USA) at 37 ο C under 5% CO 2 atmosphere. 9,10,36 To observe NGF bioactivity after release of NGF at scheduled time, PC-12 cells were plated at a density of 8 Ý 10 4 cells/cm 2 onto the NGF-loaded PLGA films eliminated with NGF released aqueous solution in 12-well plate for 7 days. After incubation at 37 ο C under 5% CO 2 atmosphere, the PLGA surfaces were washed with PBS and the PC-12 cells attached on the surfaces were fixed with 2.5% glutaraldehyde (GIBCO BLR) in PBS for 24 hrs at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, 9,10,36 we studied NGF-loaded PLGA devices such as microspheres and scaffolds for the possibility of the application of neural tissue engineering. One of the significant disadvantages is the massive initial burst from the surface of NGF loaded microspheres and interconnected pores of the PLGA scaffolds and the massive loss during the preparation process resulting in no detection of accurate NGF concentration.…”

Section: Introductionmentioning

confidence: 99%

Controlled release of nerve growth factor from sandwiched poly(L-lactide-co-glycolide) films for the application in neural tissue engineering

et al. 2003

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In order to fabricate new sustained delivery device of nerve growth factor (NGF), we developed NGFloaded biodegradable poly(L-lactide-co-glycolide) (PLGA, the mole ratio of lactide to glycolide 75:25, molecular weight: 83,000 and 43,000 g/mole, respectively) film by novel and simple sandwich solvent casting method for the possibility of the application of neural tissue engineering. PLGA was copolymerized by direct condensation reaction and the molecular weight was controlled by reaction time. Released behavior of NGF from NGF-loaded films was characterized by enzyme linked immunosorbent assay (ELISA) and degradation characteristics were observed by scanning electron microscopy (SEM) and gel permeation chromatography (GPC). The bioactivity of released NGF was identified using a rat pheochromocytoma (PC-12) cell based bioassay. The release of NGF from the NGF-loaded PLGA films was prolonged over 35 days with zero-order rate of 0.5~0.8 ng NGF/day without initial burst and could be controlled by the variations of molecular weight and NGF loading amount. After 7 days NGF released in phosphate buffered saline and PC-12 cell cultured on the NGF-loaded PLGA film for 3 days. The released NGF stimulated neurite sprouting in cultured PC-12 cells, that is to say, the remained NGF in the NGF/PLGA film at 37 ο C for 7 days was still bioactive. This study suggested that NGF-loaded PLGA sandwich film is released the desired period in delivery system and useful neuronal growth culture as nerve contact guidance tube for the application of neural tissue engineering.

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The effect of surface wettability on induction and growth of neurites from the PC-12 cell on a polymer surface

Cited by 141 publications

References 33 publications

Biomimetic micro/nanostructured functional surfaces for microfluidic and tissue engineering applications

Biomimetic micro/nanostructured functional surfaces for microfluidic and tissue engineering applications

Neuronal Differentiation Modulated by Polymeric Membrane Properties

Controlled release of nerve growth factor from sandwiched poly(L-lactide-co-glycolide) films for the application in neural tissue engineering

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