The effect of surface properties on bacterial retention: A study utilising stainless steel and TiN/25.65at.%Ag substrata

Tetlow, Louise; Lynch, Stephen; Whitehead, Kathryn A.

doi:10.1016/j.fbp.2017.01.011

Cited by 10 publications

(10 citation statements)

References 32 publications

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“…Before biofilm formation occurs, the microorganisms will bind to the surface in a series of steps ( Tetlow et al, 2017 ). The initial step, which is governed by physicochemical forces, is the attachment stage, and microorganisms may be easily removed from the surface at this point.…”

Section: Discussionmentioning

confidence: 99%

Diverse surface properties reveal that substratum roughness affects fungal spore binding

et al. 2021

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Summary Binding to surfaces by fungal spores is a prerequisite to biofilm formation. The interactions of polytetrafluoroethylene (PTFE), glass, and silicon with three fungal spores, of differing shapes and sizes ( Aspergillus niger 1957, Aspergillus niger 1988, and Aureobasidium pullulans ), were investigated. A multifractal analysis was conducted to provide quantitative measures of density, dispersion, and clustering of spores on the surfaces. The PTFE, glass, and silicon surfaces presented a range of surface topographies and wettabilities. PTFE was the roughest and most non-wettable surface, whereas silicon was the opposite in terms of both these aspects. The A. niger species were more non-wettable than A. pullulans . Overall, A. niger 1957 attached in higher numbers to PTFE, whereas A. niger 1988 and A. pullulans bound in highest numbers to glass. The results of this work demonstrated that the overall substratum surface roughness influenced spore binding rather than the physicochemical or chemical properties of surfaces or spores.

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Section: Discussionmentioning

confidence: 99%

Diverse surface properties reveal that substratum roughness affects fungal spore binding

et al. 2021

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“…The AFM showed a heterogeneous surface topography where the PVAc had a more irregular surface topography than the PVOH. The main parameter often reported in the literature for attachment and adhesion of microorganisms is surface roughness, and this may be evaluated according to R or S values [46,51]. It has been suggested that a rougher surface may enhance the attachment and adhesion of fungi, due to the greater surface area and therefore more available surface sites for thermodynamic reactions to occur [52,53].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Surface Hydrophobicity on the Attachment of Fungal Conidia to Substrates of Polyvinyl Acetate and Polyvinyl Alcohol

et al. 2020

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Biofouling of PVAc and PVOH surfaces by fungal conidia can result in surface discolouration and subsequent biodeterioration. In order to understand the interactions of fungal conidia on polymer surfaces, the surface properties of PVAc and PVOH and the hydrophobicity, size and shape of three type of fungal conidia was determined (Aspergillus niger 1957, Aspergillus niger 1988 and Aureobasidium pullulans). Fungal conidia were used in a range of binding assays (attachment, adhesion and retention). The PVAc and PVOH demonstrated different surface topographies and the PVAc demonstrated a higher maximum height (300.6 nm) when compared to the PVOH (434.2 nm). The PVAc surfaces was less wettable (75°) than the PVOH surface (62°). The FTIR demonstrated differences in the chemistries of the two surfaces, whereby the PVOH confirmed the presence of polar moieties. Hydrophobicity assays demonstrated that both A. niger species' were more non-wettable than the A. pullulans. Following the attachment assays, the more hydrophobic Aspergillus spp. conidia attached in greater numbers to the more wettable surface and the A. pullulans was retained in greater numbers to the less wettable PVAc surface. The adhesion and retention assays demonstrated that the more polar surface retained all the types of conidia, regardless of their surface hydrophobicities. This study demonstrated that conidial binding to the surfaces were influenced by the chemistry and physicochemistry of the surfaces and spores. However, the inclusion of a washing stage influenced the adhesion of conidia to surfaces. In environments that were indicative of a attachment or retention assay a PVAc surface would reduce the number of A. niger spp. spores whilst a PVOH surface would reduce the number of A. pullulans spores. However, in an environment similar to a adhesion assay, a PVAc surface would be most beneficial to reduce spore retention. Thus, the use of the correct methodology that reflects the environment in which the surface is to be used is important in order to accurately inform hygienic surface development.

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“…A Δf(α) < 0 indicated more intense clustering of the MoO 2 . 25,40 Therefore, MFA revealed that clusters of gaps were dominant up to 60 s, and that clusters of MoO 2 were observed for 240 and 300 s, indicating a time-dependent deposition process. Interestingly, as the MoO 2 deposition time increased, the MoO 2 on the AM-G M s became more agglomerated; this strengthens the theory that MoO 2 selectively bound to the electrochemically active sites of the AM-G M s. Thus, MFA, which relies upon the estimation of fractional features, 41 was successfully used in this study; the fractional features were correlated with MoO 2 depositions.…”

Section: ■ Results and Discussionmentioning

confidence: 95%

“…A Δ f (α) > 0 indicated a cluster of gaps and therefore more space between the MoO 2 on the surface of the electrode. , As the deposition time of MoO 2 increased, the clustering value became more negative, with the first negative value reported at 180 s (−0.055) and the most negative value was observed at the 300 s MoO 2 deposition time point. A Δ f (α) < 0 indicated more intense clustering of the MoO 2 . , Therefore, MFA revealed that clusters of gaps were dominant up to 60 s, and that clusters of MoO 2 were observed for 240 and 300 s, indicating a time-dependent deposition process. Interestingly, as the MoO 2 deposition time increased, the MoO 2 on the AM-G M s became more agglomerated; this strengthens the theory that MoO 2 selectively bound to the electrochemically active sites of the AM-G M s. Thus, MFA, which relies upon the estimation of fractional features, was successfully used in this study; the fractional features were correlated with MoO 2 depositions.…”

Section: Resultsmentioning

confidence: 98%

“…A Δf(α) > 0 indicated a cluster of gaps and therefore more space between the MoO 2 on the surface of the electrode. 25,40 As the deposition time of MoO 2 increased, the clustering value became more negative, with the first negative value reported at 180 s (−0.055) and the most negative value was observed at the 300 s MoO 2 deposition time point. A Δf(α) < 0 indicated more intense clustering of the MoO 2 .…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Electrochemical Decoration of Additively Manufactured Graphene Macroelectrodes with MoO₂ Nanowires: An Approach to Demonstrate the Surface Morphology

et al. 2020

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Additive manufacturing (AM) provides a unique platform for the rapid design and fabrication of complex structures. Printed structures can be used as is or as templates to be decorated with electrochemical deposited nanomaterials, which may be utilised as electrocatalytic sensing platforms. Novel methods are required to determine the electrochemical deposited morphology present on the electrode surfaces. Additively manufactured graphene macroelectrodes (AM-GMs) were fabricated using a commercially available feedstock and molybdenum (di)oxide (MoO2) was successfully electrochemically deposited onto the electrode surface. The electrochemically deposited MoO2 was analysed using scanning electron microscopy (SEM), optical interferometry, Raman spectroscopy and multifractal analysis (MFA). Although the electrochemical deposition of MoO2 nanowires were clearly visible using SEM, MFA enabled quantification of the MoO2 nanowires, deposited at a variety of time points (20 -300 s). MFA was utilised to generate quantitative data, derived from ƒ(α) curves, to determine the area of the electrochemical deposited MoO2 nanowires, including coverage, density, dispersion and clustering. The AM-GMs which were subjected to 300 s (maximum time period) of MoO2 electrodeposition demonstrated the greatest percentage area coverage (20.14 %). The use of such mathematical systems offers an inexpensive method to characterise the parameters of electrochemically-deposited materials.

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The effect of surface properties on bacterial retention: A study utilising stainless steel and TiN/25.65at.%Ag substrata

Cited by 10 publications

References 32 publications

Diverse surface properties reveal that substratum roughness affects fungal spore binding

Diverse surface properties reveal that substratum roughness affects fungal spore binding

The Effect of Surface Hydrophobicity on the Attachment of Fungal Conidia to Substrates of Polyvinyl Acetate and Polyvinyl Alcohol

Electrochemical Decoration of Additively Manufactured Graphene Macroelectrodes with MoO₂ Nanowires: An Approach to Demonstrate the Surface Morphology

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The effect of surface properties on bacterial retention: A study utilising stainless steel and TiN/25.65at.%Ag substrata

Cited by 10 publications

References 32 publications

Diverse surface properties reveal that substratum roughness affects fungal spore binding

Diverse surface properties reveal that substratum roughness affects fungal spore binding

The Effect of Surface Hydrophobicity on the Attachment of Fungal Conidia to Substrates of Polyvinyl Acetate and Polyvinyl Alcohol

Electrochemical Decoration of Additively Manufactured Graphene Macroelectrodes with MoO2 Nanowires: An Approach to Demonstrate the Surface Morphology

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Product

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About

Electrochemical Decoration of Additively Manufactured Graphene Macroelectrodes with MoO₂ Nanowires: An Approach to Demonstrate the Surface Morphology