The effect of surface active agents on the permeability to dye of the plasma membrane of ehrlich ascites cells

Hodes, M. E.; Palmer, Catherine G.; Warren, Aaron K.

doi:10.1016/0014-4827(60)90357-8

Cited by 27 publications

(10 citation statements)

References 7 publications

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“…Albumin (0.1 %) had no effect on the determina- The average MIC decreased from a maximum for the C6 and C20 (10 and 3.2 ,umoles/ml, respectively) fatty acids to a minimum of 1. 16 Amoles/ ml (range, 0.062 to 2.49) for C12. The greatest bacteriostatic activity was found for lauric acid (C12).…”

Section: Resultsmentioning

confidence: 98%

“…From Table 5, it can be seen that only monolaurin was more active than the free acid. The antitumor activity of monoglycerides, especially monolaurin (17), suggests that these compounds may affect some fundamental process of cellular growth: Hodes and associates (15,16) have reported detailed studies of the mode of action of synthetic surfactants on tumor cell membranes. Similar compounds have been found which reduce respiration of ascites cells (20) and their incorporation of 32p (7).…”

Section: Discussionmentioning

confidence: 99%

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Fatty Acids and Derivatives as Antimicrobial Agents

Kabara

Swieczkowski

Conley

et al. 1972

Antimicrob Agents Chemother

766

614

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The structural relationships of 30 straight-chain fatty acids and derivatives and their bactericidal properties were studied with 8 gram-negative and 12 grampositive organisms. Chain length, unsaturation (cis, trans), and functional group were all variables considered in this study. Our data indicate that C12 (lauric acid) is the most inhibitory saturated fatty acid against gram-positive organisms. Monoenoic acid (C18:l) was more inhibitory than saturated fatty acid, but was less active than dienoic derivatives (C182). Other unsaturated compounds were less acti-e than C182. Alcohols and glyceryl esters were active only against gram-positive organisms. In general, esterification of the carboxyl group led to a compound which was less active; monoglycerides were the sole exception. Amine derivatives, contrary to results with fatty acids, esters, and amides, showed activity against both gram-positive and gram-negative organisms.The antifungal and bactericidal properties of fatty acids are well known (5,12,22 Standard solutions (suspensions) at 1,000 ,ug/ml were twofold serially diluted with additional broth to a concentration of 125 ,ug/ml. A 4-ml amount of each dilution was then quickly, and with constant agitation, dispensed into appropriately labeled screw-capped glass tubes (16 by 125 mm). The tubes were sterilized with steam at 15 psi for 15 min. After sterilization, all tubes were cooled and incubated overnight at 35 C to insure sterility.If a compound was found to have inhibitory activity at concentrations lower than 125 ,g/ml, the testing was continued to a final concentration of 1.5 ,ug/ml for each active compound. All compounds were compared on a molar (micromoles/milliliter) rather than on a mass basis.

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Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Fatty Acids and Derivatives as Antimicrobial Agents

Kabara

Swieczkowski

Conley

et al. 1972

Antimicrob Agents Chemother

766

614

View full text Add to dashboard Cite

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“…HAEMOLYSIS as a manifestation of the cytolytic activity of surface active agents was early recognized to be a consequence of cell membrane damage (Valko, 1946;Hotchkiss, 1946;Bennett and Connon, 1957;Hodes et al, 1960). The nature of such damage must of course be directly related to the structural composition of the membranes, about which an ever-increasing amount of information has become available (Fishman, 1962;Van Deenen, 1965;Kumerow, 1965;Biochimica et Biophysica Acta, 1967, 1969Northcote, 1968 andChapman, 1968).…”

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confidence: 99%

“…Leakage of N-containing substances out of the attacked cells could be observed (Hotchkiss, 1946), probably due to the removal of soluble nucleotides and amino acids (Kay, 1965). Furthermore, the proper detergent is able to combine with the membrane proteins aind/or lipids (Valko, 1946;Ponder, 1948 andHodes et al, 1960). As to the latter membrane components, phospholipids are, at least in part, released from the cell membrane by detergents (Rideal and Taylor, 1957) as also happens with cholesterol (Barrett and Hodes, 1960).…”

mentioning

confidence: 99%

“…As to the latter membrane components, phospholipids are, at least in part, released from the cell membrane by detergents (Rideal and Taylor, 1957) as also happens with cholesterol (Barrett and Hodes, 1960). It is understandable, then, that by increasing the concentration of the detergent, the cell membrane gradually increases in permeability (Hober and Hober, 1942;Kaltenbach et al, 1958;Hodes et al, 1960) or " becomes thinner " (Yamada et al, 1963) which finally leads to the complete disintegration (lysis) of the cell as a whole (Kishimoto and Adelman, 1964).…”

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Growth Inhibition of Tumour Implants by Associated Surface Active Agents

Altman¹,

Spoladore²,

Esch³

1970

Br J Cancer

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SUMMARY.-Whereas dilute solutions of surface active agents modify the properties of cell membranes, particularly in relation to their electrical behaviour, moderate and strong solutions provoke more serious structural damage of the membrane, leading to an increase of its permeability and, finally, to cytolysis. These phenomena have inspired some authors to apply detergents as possible cancer chemotherapeuticals so far, however, with only poor results. The disintegrating effect of tumour emboli into single cells by certain detergents, and the ingenious discovery that the mutual adhesiveness between cancer cells is much less than between normal cells, have led the present authors to investigate the action of some biological surface active agents, alone as well as in some of their associations on the " take " of Yoshida sarcoma implants. Certain associations showed, in contradistinction to the separately applied components, surprisingly favourable activity. It could be established that a correlation actually exists between inhibitory effect and surface activity.

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The effect of non‐ionic detergent tween 80 on colcemid‐resistant transformed mouse cells in vitro

Stavrovskaya¹,

Потапова²,

Rosenblat³

et al. 1975

Intl Journal of Cancer

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The effect of the non-ionic detergent tween 80 on one colcemid-resistant and one sensitive subline of mouse L cells has been studied. The colcemid-resistant subline was also resistant to colchicine and Vinca alkaloids. Tween 80 at concentrations of 0.01% (v/v) or higher increased the sensitivity of drug-resistant cells to the antimitotic effect of colcemid, colchicine and vinblastine. Tween 80 also potentiated the initial rate and the maximal level of the (3-H)-cholchicine uptake by both sensitive and resistant cells. However, the detergent at concentrations of up to 1% had no effect on (3-H)-colchicine binding by cell homogenates. Thus it appears that the effects of tween 80 were due to an increase in cell membrane permeability to the drugs. The effect was completely reversible. The cells did not become adapted to the sensitizing action of tween 80 even after prolonged incubation in medium containing the detergent. A considerable increase in the permeability to the drug was obtained with doses of tween 80 which were non-toxic and which had no effect on cell proliferation, morphology and locomotion. The increase in membrane permeability caused by tween 80 at these concentrations was selective, the membrane permeability to ions and to (3-H)-2-deowy-D-glucose being, in fact, unchanged.

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The effect of surface active agents on the permeability to dye of the plasma membrane of ehrlich ascites cells

Cited by 27 publications

References 7 publications

Fatty Acids and Derivatives as Antimicrobial Agents

Fatty Acids and Derivatives as Antimicrobial Agents

Growth Inhibition of Tumour Implants by Associated Surface Active Agents

The effect of non‐ionic detergent tween 80 on colcemid‐resistant transformed mouse cells in vitro

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