The effect of supplementation of Lactobacillus paracasei HII01 on salivary cortisol, and dehydroepiandrosterone sulfate (DHEA-S) levels

Abstract

Background of the study: Fatigue is one of the serious health issues, and stress is the main factor that induces chronic fatigue syndrome (CFS). The dysregulation of stress management pathway may account for the development of CFS. The human body comprises several neurobiological networks to manage physical and emotional insults. Hypothalamic-pituitary- adrenal (HPA) axis is one of the important neuroendocrine networks involved in the neurophysiological activity of the host system. The response of HPA axis depends on the physical and psychological state of stress and other factors like time and duration of stress. The probiotic supplements are proved as an adjuvant therapeutic agent for several diseases. 
 Aims and Objective: The aim of the current study was to evaluate the effect of Lactobacillus paracasei HII01 supplementation on salivary cortisol and DHEA-S levels of fatigue subjects. 
 Materials and Methods: After 12-weeks of probiotic intervention significantly reduced the salivary cortisol level, while DHEA-S level was not affected. The ratio of cortisol: DHEA-S was reduced after probiotic intervention. 
 Results: The results concluded that L. paracasei HII01 has the ability to reduce the stress level in fatigue subjects by reducing the salivary cortisol level.
 Conclusion: The results evidently to recommend that the ingestion of 12.5 billion cells of L. paracasei HII01 per day for 12 weeks significantly amended HPA-Axis by lowering salivary cortisol and ratio cortisol: DHEA-S in fatigue subjects.

A systematic review and meta-analysis of probiotic consumption and metabolic status of athletes

“…Our meta-analysis revealed no beneficial effect for probiotic consumption on cortisol level, which is in agreement with previous works. [1,3,17] In contrast to our findings, Lalitsuradej et al [33] reported that 12 weeks of probiotic supplementation (L. paracasei HII01) decreased salivary cortisol. Similarly, Messaoudi et al [34] and Sawada et al [35] also indicated that probiotic supplementation could attenuate urine free cortisol and salivary cortisol levels.…”

Neurobiological effects of a probiotic-supplemented diet in chronically stressed male Long-Evans rats: Evidence of enhanced resilience

“…The absence of a PB effect on baseline measures before the animals were exposed to stress, however, suggest that probiotics may be more advantageous in psychologically challenging circumstances than in less stressful conditions ( Papalini et al, 2018 ). These results corroborate recent findings indicating that Lactobacillus paracasei H1101 decreased the salivary CORT/DHEA metabolites in humans ( Lalitsuradej et al, 2020 ), suggesting that the results have potential for translational relevance. Thus, the current endocrine findings provide additional support that the gut microbiome may play a regulatory role in the stress response ( Karl et al, 2018 ), providing a potential therapeutic window for the treatment of stress-related psychiatric illnesses.…”

Psychobiotic Supplementation of PS128TM Improves Stress, Anxiety, and Insomnia in Highly Stressed Information Technology Specialists: A Pilot Study

“…We measured salivary cortisol, α-amylase ( 50 ), immunoglobulin A (IgA) ( 51 ), lactoferrin ( 52 ), and lysozyme ( 51 ) levels since they were found to be related to stress and anxiety. A recent study showed that the injection of probiotics for 12 weeks significantly reduced the level of salivary cortisol in nine fatigue subjects ( 53 ). Salivary α-amylase, IgA, lactoferrin, and lysozyme levels were measured by enzyme-linked immunosorbent assay kits (Immuno-Biological Laboratories, Inc., USA; Germany; and Assaypro LLC, USA).…”