The Effect of Sulforaphane on the Activity and Mineralization of Osteoblasts under Oxidative Stress

Abstract

Sulforaphane (SFN) is considered an antioxidant agent, but the biological effects on hypoxia-treated osteoblasts remain unclear. Therefore, the aims of this study were to investigate the effects of SFN on the activity and mineralization of osteoblasts in hypoxia. Osteoblasts were treated with hypoxia with or without SFN, and apoptosis was assayed with caspase 3 Activity Assay Kit and flow cytometer. The levels of reactive oxygen species (ROS) were measured with DCFH-DA. The levels of glutathione (GSH) and glutathione disulphide were determined by the o-phthalaldehyde fluorimetric assay. Mineralization of Osteoblasts was detected by Alizarin red staining and alkaline phosphatase (ALP) staining, and the relative proteins levels were examined by Western blotting. Our results showed that SFN reduced the hypoxia-mediated apoptosis and ROS levels in osteoblasts. The utilization of SFN improved the inhibitory effect of osteoblast mineralization by hypoxia. Additionally, the effect of alleviating hypoxia by SFN will be an increase in osteoblast activity. These findings clarify the effects of SFN on hypoxia-treated osteogenesis and will help identify novel therapeutic strategies for the protection of skeletal health.

Sulforaphane protects human umbilical vein endothelial cells from oxidative stress via the miR‑34a/SIRT1 axis by upregulating nuclear factor erythroid‑2‑related factor 2

“…Yoon et al (14) previously demonstrated that SFN pretreatment protects against ischemia and reperfusion-induced acute renal failure in rats by suppressing oxidative stress in renal tissues. Furthermore, Chen et al (15) reported that SFN reduces ROS levels and protects osteoblasts from apoptosis in vitro, whilst Zhu et al (16) suggested that SFN prevents rat aortic smooth muscle cells death by inhibiting ROS production and oxidative cytotoxicity induced by xanthine oxidase. However, to the best of our knowledge, the potential effects of SFN on vascular endothelial cells under oxidative stress have not been previously reported.…”

Controlled delivery of procyanidin through magnesium oxide nanoparticles (MgO NPs) to improve the activity and mineralization of osteoblasts under oxidative stress in vitro

“…However, excessive ROS block and reduce the activity and differentiation of osteoblast, therefore inhibit the mineralization and osteogenesis [6]. Thus, using antioxidants for ROS scavenging benefits mineralization of osteoblasts [31].…”