The effect of strong static magnetic field on lymphocytes

Aldinucci, Carlo; Garcia, Julian Blanco; Palmi, Mitri; Sgaragli, Gian Pietro; Benocci, Alberto; Meini, Antonella; Pessina, Federica; Rossi, Claudio; Bonechi, Claudia; Pessina, G. P.

doi:10.1002/bem.10071

Cited by 78 publications

(44 citation statements)

References 35 publications

(35 reference statements)

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“…In a previous report, in which these conditions were similar to those in our present study, an exposure of 4.75 T for up to 48 h had no proliferative, activating, or proinflammatory effects on peripheral blood MNCs. 31 There are possible limitations to this study. First, in this study, the effect of EMF itself on the repair process of injured muscle was not examined.…”

Section: Discussionmentioning

confidence: 92%

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Ohkawa

Kamei²,

Kamei³

et al. 2013

Tissue Engineering Part C: Methods

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Skeletal muscle injuries often leave lasting functional damage or pain. Muscle injuries are routinely treated conservatively, but the most effective treatment to promote the repair of injured muscles has not yet been established. Our previous report demonstrated that human peripheral blood-derived CD133+ cell transplantation to rat skeletal muscle injury models inhibited fibrosis and enhanced myogenesis after injury. However, the acquisition of a sufficient number of cells remains the limitation for clinical application, as the CD133 + population is rare in human blood. In this study, we applied a magnetic cell targeting system to accumulate transplanted cells in the muscle injury site and to enhance the regenerative effects of CD133 + cell transplantation, focusing on the fact that CD133 + cells are labeled with a magnetic bead for isolation. For the magnetic cell targeting, the magnet field generator was set up to adjust the peak of the magnetic gradient to the injury site of the tibialis anterior muscle, and 1 · 10 4 human peripheral blood CD133 + cells were locally injected into the injury site. This cell number is 10% of that used in the previous study. In another group, the same number of CD133 + cells was injected without magnetic force. The CD133 + cells transplanted with the magnetic force were more accumulated in the muscle injury site compared with the CD133 + cells transplanted without the magnetic force. In addition, the transplantation of CD133+ cells under the magnetic control inhibited fibrous scar formation and promoted angiogenesis and myogenesis, and also upregulated the mRNA expression of myogenic transcription factors, including Pax7, MyoD1 and Myogenin. However, the transplantation of CD133 + cells without the magnetic force failed to demonstrate these effects. Thus, our magnetic cell targeting system enables transplantation of a limited number of CD133 + cells to promote the repair of skeletal muscle injury.

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Section: Discussionmentioning

confidence: 92%

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Ohkawa

Kamei²,

Kamei³

et al. 2013

Tissue Engineering Part C: Methods

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“…45 Finally, Aldinucci and colleagues demonstrated that magneticˆelds decrease the levels of interleukin-2 and Ca 2＋ in Jurkat cells but did not aŠect concentrations of interleukin-1b, -2, and -6, interferon, and tumor necrosis factor a in normal human lymphocytes. 46 These reports indicate diŠerences between normal and cancer cells in sensitivity to magneticˆelds.…”

Section: Recent Medical Applications That Use Electromagnetic Fieldsmentioning

confidence: 99%

Biological Effects of Electromagnetic Fields and Recently Updated Safety Guidelines for Strong Static Magnetic Fields

Yamaguchi-Sekino

Sekino

Ueno

2011

MRMS

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Humans are exposed daily to artiˆcial and naturally occurring magneticˆelds that originate from many diŠerent sources. We review recent studies that examine the biological eŠects of and medical applications involving electromagneticˆelds, review the properties of static and pulsed electromagneticˆelds that aŠect biological systems, describe the use of a pulsed electromagneticˆeld in combination with an anticancer agent as an example of a medical application that incorporates an electromagneticˆeld, and discuss the recently updated safety guidelines for static electromagneticˆelds. The most notable modiˆcations to the 2009 International Commission on Non-Ionizing Radiation Protection guidelines are the increased exposure limits, especially for those who work with or near electromagnetiĉ elds (occupational exposure limits). The recommended increases in exposure were determined using recent scientiˆc evidence obtained from animal and human studies. Several studies since the 1994 publication of the guidelines have examined the eŠects on humans after exposure to high static electromagneticˆelds (up to 9.4 tesla), but additional research is needed to ascertain further the safety of strong electromagneticˆelds.

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“…It has been shown that static HMF under 5 T has little or no effect on cell growth (1,2). Depending on the cell type, different influences of HMF on apoptosis have been reported (3)(4)(5)(6).…”

mentioning

confidence: 99%

“…Depending on the cell type, different influences of HMF on apoptosis have been reported (3)(4)(5)(6). HMF may also perturb Ca 2ϩ signaling in cells, although this is also dependent on the cell type (1,6,7).…”

mentioning

confidence: 99%

Cellular disorders induced by high magnetic fields

Valiron

Peris

Rikken

et al. 2005

Magnetic Resonance Imaging

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Purpose: To evaluate whether static high magnetic fields (HMFs), in the range of 10 -17 T, affect the cytoskeleton and cell organization in different types of mammalian cells, including fibroblasts, epithelial cells, and differentiating neurons.Materials and Methods: Cells were exposed to HMF for 30 or 60 minutes and subsequently assessed for viability. Cytoskeleton arrays and focal adhesions were visualized using immunofluorescence microscopy.Results: Cell exposure to HMF over 10 T in the case of cycling cells, and over 15 T in the case of neurons, affected cell viability, apparently because of cell detachment from culture dishes. In the remaining adherent cells, the organization of actin assemblies was perturbed, and both cell adhesion and spreading were impaired. Moreover, in the case of neurons, exposure to HMF induced growth cone retraction and delayed cell differentiation. Conclusion:Cell exposure to HMF (over 10T and 15 T in the case of cycling cells and neurons, respectively) affects the cell cytoskeleton, with deleterious effects on cell viability, organization, and differentiation. Further studies are needed to determine whether such perturbations, as observed here in cultured cells, have consequences in whole animals.

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The effect of strong static magnetic field on lymphocytes

Cited by 78 publications

References 35 publications

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Biological Effects of Electromagnetic Fields and Recently Updated Safety Guidelines for Strong Static Magnetic Fields

Cellular disorders induced by high magnetic fields

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The effect of strong static magnetic field on lymphocytes

Cited by 78 publications

References 35 publications

Magnetic Targeting of Human Peripheral Blood CD133+Cells for Skeletal Muscle Regeneration

Magnetic Targeting of Human Peripheral Blood CD133+Cells for Skeletal Muscle Regeneration

Biological Effects of Electromagnetic Fields and Recently Updated Safety Guidelines for Strong Static Magnetic Fields

Cellular disorders induced by high magnetic fields

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Resources

About

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration