The effect of Streptomycin on antibody synthesis in vitro.

Abstract

Streptomycin inhibits protein synthesis in bacteria by interfering with ribosomal function. SM\1 inhibited the incorporation of amino acids in undefined cell-free systems from Escherichia colil 2 and decreased polyphenylalanine synthesis in E. coli ribosomes when poly U was used as messenger." It was suggested that SMAI acts by interfering with either the attachment or functioning of poly U on the ribosome. SMl did not prevent attachment of poly U to ribosomes, but the presence of poly U on ribosomes made the SM binding site less accessible to the antibiotic. This binding site is on the 30S ribosomal subunit, the one to which messenger also binds.7 Davies et al.8 showed that in E. coli ribosomes with poly U as messenger, SM decreased incorporation of phenylalanine and increased incorporation of isoleucine. Thus, SM altered the translation process whereby the information of the messenger was decoded. Davies et al.8 suggested that S\I acts on intact bacteria, not by inhibiting protein synthesis, but by causing the synthesis of nonfunctional proteins with altered amino acid sequences. Pestka et al.9 have recently

Symposium on in vitro studies of the immune response. IV. Role of nucleic acids in the anamnestic antibody response.

“…Such roles would be consistent with the findings of amino acid differences between antibodies of different specificities (21) and with data for the colinearity of gene structure and the amino acid sequence of its protein end product (48). Recently, evidence has been adduced for in vitro synthesis of altered antibodies with changed specificity upon addition of streptomycin to lymphoid cultures (22). Presumably, the streptomycin introduced amino acid changes into the antibody molecule by altering the translation process whereby the messenager RNA is decoded at the ribosomal level.…”

Antibiotic inhibitors of the bacterial ribosome.

“…Erythromycin exerts a similar inhibitory effect on chloroplasts in Euglena (46). Streptomycin has been reported to interfere with the production of antibodies in vitro, producing antibodies with altered serological activity (97). However, it is not clear whether this effect is due to a direct action on protein synthesis or to the formation of a streptomycin-antigen complex (98).…”

History of Cell Culture

“…Keilova presented in her work influence of streptomycin directly on the explants of heart, aorta and frontal bone of the chick embryo [84]. It was also found by Lawrence that in higher concentration, antibiotics (including penicillin, streptomycin, tetracycline and neomycin) affected not only migration of epithelium around skin explants, but also in some concentrations caused respiratory damage or necrotic changes [83]. In other study, Krueger analyzed effect of streptomycin on protein synthesis in mammalian cells and found that this antibiotic altered the in vitro synthesis of antibody to phage MS-2 in spleen and lymph node cells from immunized rabbits [85].…”

“…Herrell and coworkers found that the different preparation of penicillin exhibited toxic action on mitosis due to some impurities in penicillin preparation. In the comparison with penicillin G, it was practically harmless for cells [82,83]. Keilova presented in her work influence of streptomycin directly on the explants of heart, aorta and frontal bone of the chick embryo [84].…”