The effect of storing whole blood at 22°C for up to 24 hours with and without rapid cooling on the quality of red cell concentrates and fresh‐frozen plasma

Abstract

These data suggest that RCCs and FFP produced from WB that has been stored at ambient temperature with or without active cooling are of acceptable quality compared with those produced using current standard methods in the United Kingdom.

The Effect of Holding Times of Whole Blood and Its Components During Processing on In Vitro and In Vivo Quality

“…This has consistently been reported in numerous studies [13][14][15][16][17][18]. Other parameters also remain more or less unaffected by the preprocessing holding time.…”

“…Hemolysis was marginally lower on day 1 and throughout storage in red cell concentrates from units held for 8 hours. Others mainly confirmed these in vitro measures [15], a comparison of less than 8-vs 24-hour hold at room temperature resulted in significantly lower hemolysis for the short-held group, throughout subsequent storage. Despite no difference at onset, free K + was higher from day 14 onward in the short-held units, possibly due to a higher temperature during processing.…”

“…However, evidence needs to be gathered in a phase IV trial to determine whether this is also true for PRP-derived platelet concentrates. [5,6,[13][14][15][16][17][18][19][20] 2,3-DPG concentration Decreases by two-thirds. Also decreases by~10% in units processed on the day of collection.…”

“…Fresenius and MacoPharma filters showed the same trend. Also, others found the best leukoreduction in red cells processed on the day of collection within 8 hours of collection, whereas those held for 24 hours sometimes had values that were "higher than expected," but nevertheless conformed to the requirement that 95% of the units contained less than 5 × 10 6 white cells [15]. Others found no effect of the holding time on white cell reduction, using a filter for red cell concentrates, comparing 4-to 6-hour hold of whole blood vs 22-to 24-hour hold with subsequent component separation and immediate filtration of the red cell concentrates [55].…”

“…After day 14 of storage, no difference between groups. Clinical consequence probably low [6,[13][14][15][16][17][18][19][20] pH, lactate pH lower, lactate higher, but disappears during storage [13][14][15][16][17][18][19][20] Hemolysis Somewhat higher, but within specifications [13][14][15][16][17][18][19][20] Other red blood cell quality measures…”