The effect of storage and processing on antimicrobial activity of Tulbaghia violacea

Abstract

a b s t r a c t Edited by J Van StadenKeywords: Tulbaghia violacea Alliaceae Society garlic Marasmicin Allicin Antimicrobial activity Tulbaghia violacea Harv. (Alliaceae) is a perennial plant native to South Africa. Various preparations made of this plant are extensively used for the treatment of microbial infections. It was shown that the key antimicrobial compound of T. violacea is the thiosulfinate marasmicin (2,4,5,7-tetrathiaoctane-4-oxide) enzymatically formed upon tissue disruption. Antimicrobial activity of marasmicin was evaluated (MIC 4-64 μg/ml) and compared with that of allicin (MIC 4-32 μg/ml), the prominent bioactive compound of garlic (Allium sativum). An HPLC method was developed and used for quantification of marasmicin in various Tulbaghia preparations. It was found that the content of marasmicin can be dramatically affected by storage conditions and subsequent treatment of the plant material. To achieve significant antimicrobial effects, it is essential to avoid exposing the plant to elevated temperatures (e.g., during drying). To maximize marasmicin formation, it is advisable to use freshly gathered plants and homogenize them in water as finely as possible. Extracts of T. violacea should be kept at low temperatures to retain their antimicrobial activity.

Effect of Different Physical Factors on efficacy of Thevetia Peruviana leaf extract and bio-formulations

“…al. , (2015) found that heat treatment leads reduction in antimicrobial activities of Garlic ( Allium sativum ) [ 21 ].…”

Effects of light intensities and varying watering intervals on growth, tissue nutrient content and antifungal activity of hydroponic cultivated Tulbaghia violacea L. under greenhouse conditions

“…T. violacea has been shown to have similar antibacterial and antifungal activities as Allium sativum (garlic) (Motsei et al, 2003;Krstin et al, 2018). T. violacea is rich in sulphur-containing compounds including thiosulfinate marasmicin (2,4,5,7-tetrathiaoctan-4-oxide) which exhibit significant antimicrobial activities (Ranglov a et al, 2015;Takaidza et al, 2015). Kubec et al (2002) isolated (R(S)R(C))-S-(methylthiomethyl) cysteine-4-oxide from the rhizomes of T. violacea.…”

Plants Used for the Traditional Management of Cancer in the Eastern Cape Province of South Africa: A Review of Ethnobotanical Surveys, Ethnopharmacological Studies and Active Phytochemicals

“…aculeastrum Antimicrobial and antioxidant [ 82 , 98 ] 23 Sutherlandia frutescens L. R.Br. Antibacterial, antioxidant, anti-inflammatory and antidiabetic [ 141 , 142 , 143 ] 24 Tulbaghia violacea Harv Antibacterial, antifungal, antioxidant and antidiabetic [ 144 , 145 ] …”